(Linear) (Six-base) MAPSORT of: KIEE6712.seq Check: 7829 from: 1 to: 2802 KIEE6712.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2385 2746 2802 Size: 2385 361 56 Fragments arranged by size: 2385 361 56 AccI GT'mk_AC Cuts at: 0 2690 2802 Size: 2690 112 Acc65I G'GTAC_C Cuts at: 0 1747 2802 Size: 1747 1055 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2033 2338 2802 Size: 2033 305 464 Fragments arranged by size: 2033 464 305 AleI CACnn'nnGTG Cuts at: 0 784 1066 2802 Size: 784 282 1736 Fragments arranged by size: 1736 784 282 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 70 102 2802 Size: 70 32 2700 Fragments arranged by size: 2700 70 32 ApaI G_GGCC'C Cuts at: 0 2219 2802 Size: 2219 583 ApoI r'AATT_y Cuts at: 0 1594 2802 Size: 1594 1208 AvaI C'yCGr_G Cuts at: 0 466 1084 1286 1414 1742 2018 2048 2802 Size: 466 618 202 128 328 276 30 754 Fragments arranged by size: 754 618 466 328 276 202 128 30 BamHI G'GATC_C Cuts at: 0 1834 2802 Size: 1834 968 BanI G'GyrC_C Cuts at: 0 70 982 1627 1747 2479 2512 2611 2617 Size: 70 912 645 120 732 33 99 6 Cuts at: 2617 2802 Size: 185 Fragments arranged by size: 912 732 645 185 120 99 70 33 6 BanII G_rGCy'C Cuts at: 0 1422 1726 2187 2197 2219 2596 2802 Size: 1422 304 461 10 22 377 206 Fragments arranged by size: 1422 461 377 304 206 22 10 BbeI G_GCGC'C Cuts at: 0 986 2802 Size: 986 1816 BbsI GAAGACnn'nnnn_ Cuts at: 0 720 2802 Size: 720 2082 BbvCI CC'TCA_GC Cuts at: 0 1273 1752 2802 Size: 1273 479 1050 Fragments arranged by size: 1273 1050 479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 132 166 1450 1484 2802 Size: 132 34 1284 34 1318 Fragments arranged by size: 1318 1284 132 34 34 BclI T'GATC_A Cuts at: 0 1940 2802 Size: 1940 862 BlpI GC'TnA_GC Cuts at: 0 520 2802 Size: 520 2282 Bme1580I G_kGCm'C Cuts at: 0 1571 1630 1805 2219 2672 2802 Size: 1571 59 175 414 453 130 Fragments arranged by size: 1571 453 414 175 130 59 BmrI ACTGGGnnnn_n' Cuts at: 0 1072 2643 2802 Size: 1072 1571 159 Fragments arranged by size: 1571 1072 159 BmtI G_CTAG'C Cuts at: 0 218 2802 Size: 218 2584 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2049 2435 2802 Size: 2049 386 367 Fragments arranged by size: 2049 386 367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 230 343 367 613 781 2802 Size: 36 194 113 24 246 168 2021 Fragments arranged by size: 2021 246 194 168 113 36 24 Bpu10I CC'TnA_GC Cuts at: 0 1273 1752 2802 Size: 1273 479 1050 Fragments arranged by size: 1273 1050 479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1192 2802 Size: 1192 1610 BsaI GGTCTCn'nnnn_ Cuts at: 0 238 1186 2165 2466 2802 Size: 238 948 979 301 336 Fragments arranged by size: 979 948 336 301 238 BsaAI yAC'GTr Cuts at: 0 2667 2802 Size: 2667 135 BsaBI GATnn'nnATC Cuts at: 0 961 2802 Size: 961 1841 BsaHI Gr'CG_yC Cuts at: 0 983 1292 1454 2234 2566 2802 Size: 983 309 162 780 332 236 Fragments arranged by size: 983 780 332 309 236 162 BsaWI w'CCGG_w Cuts at: 0 247 2379 2802 Size: 247 2132 423 Fragments arranged by size: 2132 423 247 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 574 604 1166 1196 2802 Size: 574 30 562 30 1606 Fragments arranged by size: 1606 574 562 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 33 298 2802 Size: 33 265 2504 Fragments arranged by size: 2504 265 33 BseYI C'CCAG_C Cuts at: 0 663 1784 2751 2802 Size: 663 1121 967 51 Fragments arranged by size: 1121 967 663 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 1256 1852 2351 2426 2802 Size: 571 685 596 499 75 376 Fragments arranged by size: 685 596 571 499 376 75 BsiEI CG_ry'CG Cuts at: 0 270 2802 Size: 270 2532 BsiHKAI G_wGCw'C Cuts at: 0 1002 1726 2197 2596 2802 Size: 1002 724 471 399 206 Fragments arranged by size: 1002 724 471 399 206 BsmI GAATG_Cn' Cuts at: 0 1470 2802 Size: 1470 1332 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1283 2802 Size: 1283 1519 Bsp1286I G_dGCh'C Cuts at: 0 1002 1422 1571 1630 1726 1805 2187 2197 Size: 1002 420 149 59 96 79 382 10 Cuts at: 2197 2219 2596 2672 2802 Size: 22 377 76 130 Fragments arranged by size: 1002 420 382 377 149 130 96 79 76 59 22 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 182 1171 2385 2746 2802 Size: 182 989 1214 361 56 Fragments arranged by size: 1214 989 361 182 56 BsrBI CCG'CTC Cuts at: 0 23 465 2802 Size: 23 442 2337 Fragments arranged by size: 2337 442 23 BsrDI GCAATG_nn' Cuts at: 0 1002 1686 2712 2769 2802 Size: 1002 684 1026 57 33 Fragments arranged by size: 1026 1002 684 57 33 BsrFI r'CCGG_y Cuts at: 0 865 2509 2614 2802 Size: 865 1644 105 188 Fragments arranged by size: 1644 865 188 105 BsrGI T'GTAC_A Cuts at: 0 1070 2802 Size: 1070 1732 BssHII G'CGCG_C Cuts at: 0 473 597 751 2802 Size: 473 124 154 2051 Fragments arranged by size: 2051 473 154 124 BstAPI GCAn_nnn'nTGC Cuts at: 0 2667 2802 Size: 2667 135 BstEII G'GTnAC_C Cuts at: 0 1158 2802 Size: 1158 1644 BstXI CCAn_nnnn'nTGG Cuts at: 0 14 2343 2562 2802 Size: 14 2329 219 240 Fragments arranged by size: 2329 240 219 14 BstYI r'GATC_y Cuts at: 0 107 168 807 1834 2802 Size: 107 61 639 1027 968 Fragments arranged by size: 1027 968 639 107 61 BtgI C'CryG_G Cuts at: 0 89 440 2288 2621 2802 Size: 89 351 1848 333 181 Fragments arranged by size: 1848 351 333 181 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1095 1634 2516 2802 Size: 1095 539 882 286 Fragments arranged by size: 1095 882 539 286 BtsI GCAGTG_nn' Cuts at: 0 298 1973 2802 Size: 298 1675 829 Fragments arranged by size: 1675 829 298 DraIII CAC_nnn'GTG Cuts at: 0 1767 2802 Size: 1767 1035 EaeI y'GGCC_r Cuts at: 0 1711 1758 2345 2802 Size: 1711 47 587 457 Fragments arranged by size: 1711 587 457 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 663 2559 2802 Size: 663 1896 243 Fragments arranged by size: 1896 663 243 EcoICRI GAG'CTC Cuts at: 0 1724 2195 2594 2802 Size: 1724 471 399 208 Fragments arranged by size: 1724 471 399 208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 230 343 367 613 781 2033 2338 Size: 36 194 113 24 246 168 1252 305 Cuts at: 2338 2802 Size: 464 Fragments arranged by size: 1252 464 305 246 194 168 113 36 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1712 2802 Size: 1712 1090 EcoO109I rG'GnC_Cy Cuts at: 0 659 820 1446 1698 2215 2216 2748 2802 Size: 659 161 626 252 517 1 532 54 Fragments arranged by size: 659 626 532 517 252 161 54 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 518 1233 2802 Size: 518 715 1569 Fragments arranged by size: 1569 715 518 HaeII r_GCGC'y Cuts at: 0 986 2417 2636 2802 Size: 986 1431 219 166 Fragments arranged by size: 1431 986 219 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 651 683 757 789 1725 1757 2361 2393 Size: 651 32 74 32 936 32 604 32 Cuts at: 2393 2578 2610 2802 Size: 185 32 192 Fragments arranged by size: 936 651 604 192 185 74 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 982 2802 Size: 982 1820 KpnI G_GTAC'C Cuts at: 0 1751 2802 Size: 1751 1051 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 572 629 1367 2802 Size: 572 57 738 1435 Fragments arranged by size: 1435 738 572 57 MscI TGG'CCA Cuts at: 0 1760 2347 2802 Size: 1760 587 455 Fragments arranged by size: 1760 587 455 MslI CAynn'nnrTG Cuts at: 0 697 784 1066 1654 2542 2802 Size: 697 87 282 588 888 260 Fragments arranged by size: 888 697 588 282 260 87 MspA1I CmG'CkG Cuts at: 0 345 1756 2313 2486 2728 2802 Size: 345 1411 557 173 242 74 Fragments arranged by size: 1411 557 345 242 173 74 NaeI GCC'GGC Cuts at: 0 867 2616 2802 Size: 867 1749 186 Fragments arranged by size: 1749 867 186 NarI GG'CG_CC Cuts at: 0 983 2802 Size: 983 1819 NcoI C'CATG_G Cuts at: 0 2621 2802 Size: 2621 181 NgoMIV G'CCGG_C Cuts at: 0 865 2614 2802 Size: 865 1749 188 Fragments arranged by size: 1749 865 188 NheI G'CTAG_C Cuts at: 0 214 2802 Size: 214 2588 NruI TCG'CGA Cuts at: 0 154 2802 Size: 154 2648 PasI CC'CwG_GG Cuts at: 0 2601 2802 Size: 2601 201 PflMI CCAn_nnn'nTGG Cuts at: 0 2758 2802 Size: 2758 44 PfoI T'CCnGG_A Cuts at: 0 110 374 758 2421 2802 Size: 110 264 384 1663 381 Fragments arranged by size: 1663 384 381 264 110 PmlI CAC'GTG Cuts at: 0 2667 2802 Size: 2667 135 PpuMI rG'GwC_Cy Cuts at: 0 659 1446 1698 2802 Size: 659 787 252 1104 Fragments arranged by size: 1104 787 659 252 PspOMI G'GGCC_C Cuts at: 0 2215 2802 Size: 2215 587 PspXI vC'TCGA_Gb Cuts at: 0 1742 2802 Size: 1742 1060 PstI C_TGCA'G Cuts at: 0 2663 2802 Size: 2663 139 PvuII CAG'CTG Cuts at: 0 1756 2728 2802 Size: 1756 972 74 Fragments arranged by size: 1756 972 74 SacI G_AGCT'C Cuts at: 0 1726 2197 2596 2802 Size: 1726 471 399 206 Fragments arranged by size: 1726 471 399 206 SanDI GG'GwC_CC Cuts at: 0 659 1446 2802 Size: 659 787 1356 Fragments arranged by size: 1356 787 659 ScaI AGT'ACT Cuts at: 0 1887 2802 Size: 1887 915 SexAI A'CCwGG_T Cuts at: 0 2325 2802 Size: 2325 477 SfcI C'TryA_G Cuts at: 0 1821 2388 2659 2802 Size: 1821 567 271 143 Fragments arranged by size: 1821 567 271 143 SfoI GGC'GCC Cuts at: 0 984 2802 Size: 984 1818 SgrAI Cr'CCGG_yG Cuts at: 0 865 2802 Size: 865 1937 SmaI CCC'GGG Cuts at: 0 1416 2050 2802 Size: 1416 634 752 Fragments arranged by size: 1416 752 634 SmlI C'TyrA_G Cuts at: 0 466 1207 1742 2802 Size: 466 741 535 1060 Fragments arranged by size: 1060 741 535 466 StuI AGG'CCT Cuts at: 0 13 2249 2802 Size: 13 2236 553 Fragments arranged by size: 2236 553 13 StyI C'CwwG_G Cuts at: 0 159 2621 2802 Size: 159 2462 181 Fragments arranged by size: 2462 181 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 623 1932 2392 2802 Size: 623 1309 460 410 Fragments arranged by size: 1309 623 460 410 TatI w'GTAC_w Cuts at: 0 1070 1885 2802 Size: 1070 815 917 Fragments arranged by size: 1070 917 815 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1166 1198 2802 Size: 1166 32 1604 Fragments arranged by size: 1604 1166 32 XbaI T'CTAG_A Cuts at: 0 2167 2802 Size: 2167 635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1239 2802 Size: 1239 1563 XhoI C'TCGA_G Cuts at: 0 466 1742 2802 Size: 466 1276 1060 Fragments arranged by size: 1276 1060 466 XmaI C'CCGG_G Cuts at: 0 1414 2048 2802 Size: 1414 634 754 Fragments arranged by size: 1414 754 634 XmnI GAAnn'nnTTC Cuts at: 0 130 1216 1302 2802 Size: 130 1086 86 1500 Fragments arranged by size: 1500 1086 130 86 Enzymes that do cut: AarI AccI Acc65I AcuI AleI AloI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SanDI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BglI BglII BmgBI BsiWI BspEI BspHI BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EarI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NotI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SapI SbfI SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I ZraI