################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BfrI BglII BlnI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MflI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccI 808 GTCTAC GT^[AC][GT]AC AccIII 620 TCCGGA T^CCGGA AcsI 10 GAATTC [GA]^AATT[CT] Alw44I 1441 GTGCAC G^TGCAC AlwI 630 GGATC GGATC(4/5) AocI 496 CCTGAGG CC^T[ACGT]AGG ApaI 824 GGGCCC GGGCC^C ApaLI 1441 GTGCAC G^TGCAC ApoI 10 GAATTC [GA]^AATT[CT] AspI 1360 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1319 CCCGGG C^[CT]CG[GA]G BalI 855 TGGCCA TGG^CCA BbrPI 568 CACGTG CAC^GTG BclI 1253 TGATCA T^GATCA BfaI 1729 CTAG C^TAG BglI 1083 GCCCATCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 171 CTCCAG CTGGAG(16/14) BsaAI 568 CACGTG [CT]AC^GT[GA] BsaBI 1224 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 620 TCCGGA [AT]^CCGG[AT] BseAI 620 TCCGGA T^CCGGA BsmI 464 GCATTC GAATGC(1/-1) BsoBI 1319 CCCGGG C^[CT]CG[GA]G Bsp1407I 1277 TGTACA T^GTACA BspEI 620 TCCGGA T^CCGGA BspMI 1079 ACCTGC ACCTGC(4/8) BsrFI 1451 ACCGGC [GA]^CCGG[CT] BsrGI 1277 TGTACA T^GTACA BstEII 1110 GGTGACC G^GT[ACGT]ACC BstPI 1110 GGTGACC G^GT[ACGT]ACC Bsu36I 496 CCTGAGG CC^T[ACGT]AGG Cfr10I 1451 ACCGGC [GA]^CCGG[CT] DraIII 956 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 103 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 409 CTCTTC CTCTTC(1/4) Eco81I 496 CCTGAGG CC^T[ACGT]AGG EcoO65I 1110 GGTGACC G^GT[ACGT]ACC EcoRI 10 GAATTC G^AATTC FauI 1142 GCGGG CCCGC(4/6) FbaI 1253 TGATCA T^GATCA HaeII 588 GGCGCT [GA]GCGC^[CT] HgaI 693 GACGC GACGC(5/10) Ksp632I 409 CTCTTC CTCTTC(1/4) MaeI 1729 CTAG C^TAG MamI 1224 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 606 CAATTG C^AATTG MluNI 855 TGGCCA TGG^CCA MroI 620 TCCGGA T^CCGGA MscI 855 TGGCCA TGG^CCA MseI 374 TTAA T^TAA MunI 606 CAATTG C^AATTG NspI 935 ACATGC [GA]CATG^[CT] PflFI 1360 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 568 CACGTG CAC^GTG PmlI 568 CACGTG CAC^GTG PvuII 1527 CAGCTG CAG^CTG ScaI 77 AGTACT AGT^ACT SmaI 1319 CCCGGG CCC^GGG SspBI 1277 TGTACA T^GTACA TaqI 348 TCGA T^CGA Tru9I 374 TTAA T^TAA Tth111I 1360 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 348 TCGA T^CGA XmaCI 1319 CCCGGG C^CCGGG XmaI 1319 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BlpI 418 GCTGAGC GC^T[ACGT]AGC 591 GCTCAGC Bpu1102I 418 GCTGAGC GC^T[ACGT]AGC 591 GCTCAGC BsrBI 787 GAGCGG CCGCTC(-3/-3) 1192 GAGCGG BstAPI 510 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 955 GCACTTAGTGC CelII 418 GCTGAGC GC^T[ACGT]AGC 591 GCTCAGC DraII 823 GGGGCCC [GA]G^G[ACGT]CC[CT] 824 GGGCCCT EcoO109I 823 GGGGCCC [GA]G^G[ACGT]CC[CT] 824 GGGCCCT HinfI 835 GATTC G^A[ACGT]TC 1749 GATTC NcoI 1106 CCATGG C^CATGG 1235 CCATGG PflMI 249 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1101 CCACACCATGG PstI 43 CTGCAG CTGCA^G 1474 CTGCAG SacI 430 GAGCTC GAGCT^C 925 GAGCTC SfaNI 1229 GCATC GCATC(5/9) 1272 GCATC TfiI 835 GATTC G^A[AT]TC 1749 GATTC Van91I 249 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1101 CCACACCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwNI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I Tsp509I TspRI XcmI XmnI