(Circular) (Six-base) MAPSORT of: pFN21AE3910.seq Check: 5050 from: 1 to: 6469 pFN21AE3910 6469 bp With 182 enzymes: SgfI * March 28, 2012 11:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2814 3812 1937 Size: 877 998 4594 Fragments arranged by size: 4594 998 877 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3050 4532 5916 6184 1632 Size: 1418 1482 1384 268 1917 Fragments arranged by size: 1917 1482 1418 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 5368 6345 1203 Size: 4165 977 1327 Fragments arranged by size: 4165 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 3113 3254 3512 1166 Size: 1947 141 258 4123 Fragments arranged by size: 4123 1947 258 141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6469 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2729 2761 2729 Size: 32 6437 AlwNI CAG_nnn'CTG Cuts at: 2056 2260 3518 4212 5784 2056 Size: 204 1258 694 1572 2741 Fragments arranged by size: 2741 1572 1258 694 204 ApaI G_GGCC'C Cuts at: 2833 2833 Size: 6469 ApaLI G'TGCA_C Cuts at: 3446 4526 5682 3446 Size: 1080 1156 4233 Fragments arranged by size: 4233 1156 1080 ApoI r'AATT_y Cuts at: 1493 2015 3778 4004 4156 6265 1493 Size: 522 1763 226 152 2109 1697 Fragments arranged by size: 2109 1763 1697 522 226 152 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 3324 3794 1870 Size: 73 1381 470 4545 Fragments arranged by size: 4545 1381 470 73 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3005 3213 3248 3790 5245 Size: 325 217 627 1218 208 35 542 1455 Cuts at: 5245 618 Size: 1842 Fragments arranged by size: 1842 1455 1218 627 542 325 217 208 35 BanII G_rGCy'C Cuts at: 1391 2240 2329 2439 2510 2833 2934 3788 1391 Size: 849 89 110 71 323 101 854 4072 Fragments arranged by size: 4072 854 849 323 110 101 89 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 2198 2592 3038 3094 3692 928 Size: 971 211 88 394 446 56 598 3705 Fragments arranged by size: 3705 971 598 446 394 211 88 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4660 4694 1903 Size: 34 2723 34 3678 Fragments arranged by size: 3678 2723 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4361 5571 4361 Size: 1210 5259 BclI T'GATC_A Cuts at: 1548 3258 1548 Size: 1710 4759 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3094 5080 136 Size: 107 122 71 631 605 1422 1986 1525 Fragments arranged by size: 1986 1525 1422 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 2424 2597 3886 2424 Size: 173 1289 5007 Fragments arranged by size: 5007 1289 173 Bme1580I G_kGCm'C Cuts at: 1790 2833 3450 4530 5686 1790 Size: 1043 617 1080 1156 2573 Fragments arranged by size: 2573 1156 1080 1043 617 BmgBI CAC'GTC Cuts at: 1318 2116 3367 1318 Size: 798 1251 4420 Fragments arranged by size: 4420 1251 798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2095 2500 3369 Size: 416 53 83 399 24 678 405 869 Cuts at: 3369 4200 5158 442 Size: 831 958 1753 Fragments arranged by size: 1753 958 869 831 678 416 405 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2161 5129 1239 Size: 922 2968 2579 Fragments arranged by size: 2968 2579 922 Bpu10I CC'TnA_GC Cuts at: 2099 2234 2099 Size: 135 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4594 5459 5757 4594 Size: 865 298 5306 Fragments arranged by size: 5306 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5132 882 Size: 617 3633 2219 Fragments arranged by size: 3633 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2575 4260 493 Size: 711 1371 1685 2702 Fragments arranged by size: 2702 1685 1371 711 BsaBI GATnn'nnATC Cuts at: 3233 6463 3233 Size: 3230 3239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4276 4658 5293 275 Size: 53 83 186 936 2743 382 635 1451 Fragments arranged by size: 2743 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2625 4902 5300 5574 5721 6132 2625 Size: 2277 398 274 147 411 2962 Fragments arranged by size: 2962 2277 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2906 2936 3679 3709 1178 Size: 30 165 30 1503 30 743 30 3938 Fragments arranged by size: 3938 1503 743 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2179 2324 3687 1186 Size: 993 145 1363 3968 Fragments arranged by size: 3968 1363 993 145 BseYI C'CCAG_C Cuts at: 5672 6168 5672 Size: 496 5973 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5896 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 2580 2934 3450 4530 4615 5686 2439 Size: 141 354 516 1080 85 1071 3222 Fragments arranged by size: 3222 1080 1071 516 354 141 85 BsmI GAATG_Cn' Cuts at: 2469 3980 4073 2469 Size: 1511 93 4865 Fragments arranged by size: 4865 1511 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2329 2439 2510 2580 2833 2934 Size: 399 450 89 110 71 70 253 101 Cuts at: 2934 3450 3788 4530 4615 5686 1391 Size: 516 338 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 450 399 338 253 110 101 89 85 71 70 BspEI T'CCGG_A Cuts at: 2625 6132 2625 Size: 3507 2962 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3093 3824 844 Size: 2249 731 3489 Fragments arranged by size: 3489 2249 731 BsrBI CCG'CTC Cuts at: 1394 1823 2794 3199 4356 1394 Size: 429 971 405 1157 3507 Fragments arranged by size: 3507 1157 971 429 405 BsrDI GCAATG_nn' Cuts at: 65 1248 2140 2188 3153 4964 5138 65 Size: 1183 892 48 965 1811 174 1396 Fragments arranged by size: 1811 1396 1183 965 892 174 48 BsrFI r'CCGG_y Cuts at: 1700 1810 3456 5113 5300 1700 Size: 110 1646 1657 187 2869 Fragments arranged by size: 2869 1657 1646 187 110 BsrGI T'GTAC_A Cuts at: 96 3282 96 Size: 3186 3283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 2521 2966 794 Size: 1727 445 4297 Fragments arranged by size: 4297 1727 445 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 3115 1184 Size: 1931 4538 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6469 BstYI r'GATC_y Cuts at: 1972 3799 4166 4551 4568 6020 6106 6118 6464 Size: 1827 367 385 17 1452 86 12 346 Cuts at: 6464 1972 Size: 1977 Fragments arranged by size: 1977 1827 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2502 2502 Size: 6469 BtgI C'CryG_G Cuts at: 513 1065 2844 2872 3111 3240 513 Size: 552 1779 28 239 129 3742 Fragments arranged by size: 3742 1779 552 239 129 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2114 3630 487 Size: 683 56 888 1516 3326 Fragments arranged by size: 3326 1516 888 683 56 BtsI GCAGTG_nn' Cuts at: 3606 3985 4779 4799 3606 Size: 379 794 20 5276 Fragments arranged by size: 5276 794 379 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 3773 3773 Size: 6469 DraIII CAC_nnn'GTG Cuts at: 2966 2966 Size: 6469 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2114 5476 809 Size: 507 798 3362 1802 Fragments arranged by size: 3362 1802 798 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2545 2795 2860 Size: 54 1425 50 275 18 715 250 65 Cuts at: 2860 2875 3916 4805 6434 8 Size: 15 1041 889 1629 43 Fragments arranged by size: 1629 1425 1041 889 715 275 250 65 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2420 4399 1353 Size: 1067 1979 3423 Fragments arranged by size: 3423 1979 1067 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5056 5430 5576 387 Size: 1064 138 3467 374 146 1280 Fragments arranged by size: 3467 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2932 2437 Size: 495 5974 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 3050 4532 5129 5916 6184 1239 Size: 393 529 889 1482 597 787 268 1524 Fragments arranged by size: 1524 1482 889 787 597 529 393 268 EcoO109I rG'GnC_Cy Cuts at: 2829 2830 2829 Size: 1 6468 EcoRI G'AATT_C Cuts at: 2015 3778 4156 2015 Size: 1763 378 4328 Fragments arranged by size: 4328 1763 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 6469 FspI TGC'GCA Cuts at: 4975 4975 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 2597 5616 1678 Size: 919 3019 2531 Fragments arranged by size: 3019 2531 919 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3047 3079 3679 3711 5110 Size: 32 135 32 1475 32 600 32 1399 Cuts at: 5110 5142 5184 5216 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1475 1399 600 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3813 4059 669 Size: 988 281 1875 246 3079 Fragments arranged by size: 3079 1875 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6469 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 2611 4068 2611 Size: 1457 5012 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3201 3448 5583 5767 1305 Size: 1896 247 2135 184 2007 Fragments arranged by size: 2135 2007 1896 247 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2862 10 Size: 54 1425 343 1030 3617 Fragments arranged by size: 3617 1425 1030 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2133 3113 3239 3254 3512 4427 4786 Size: 648 967 980 126 15 258 915 359 Cuts at: 4786 4945 518 Size: 159 2042 Fragments arranged by size: 2042 980 967 915 648 359 258 159 126 15 MspA1I CmG'CkG Cuts at: 1606 2198 2821 3534 3885 4562 5710 5955 1606 Size: 592 623 713 351 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 623 592 351 245 NcoI C'CATG_G Cuts at: 513 1065 3111 3240 513 Size: 552 2046 129 3742 Fragments arranged by size: 3742 2046 552 129 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 2944 3827 5372 1132 Size: 97 1715 883 1545 2229 Fragments arranged by size: 2229 1715 1545 883 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 2260 3112 1108 Size: 1152 852 4465 Fragments arranged by size: 4465 1152 852 PfoI T'CCnGG_A Cuts at: 2910 3065 2910 Size: 155 6314 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PmlI CAC'GTG Cuts at: 2575 2575 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspOMI G'GGCC_C Cuts at: 2829 2829 Size: 6469 PstI C_TGCA'G Cuts at: 830 2052 3483 3821 830 Size: 1222 1431 338 3478 Fragments arranged by size: 3478 1431 1222 338 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 3534 3534 Size: 6469 SacI G_AGCT'C Cuts at: 2439 2934 2439 Size: 495 5974 SalI G'TCGA_C Cuts at: 1936 3811 1936 Size: 1875 4594 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 2084 4717 6429 2084 Size: 2633 1712 2124 Fragments arranged by size: 2633 2124 1712 SfcI C'TryA_G Cuts at: 826 1045 2048 2272 3479 3817 4952 5633 5824 Size: 219 1003 224 1207 338 1135 681 191 Cuts at: 5824 826 Size: 1471 Fragments arranged by size: 1471 1207 1135 1003 681 338 224 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3326 3796 1872 Size: 1454 470 4545 Fragments arranged by size: 4545 1454 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 4573 5474 5736 820 Size: 197 926 2630 901 262 1553 Fragments arranged by size: 2630 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 3111 3161 3240 3540 3908 513 Size: 552 2046 50 79 300 368 3074 Fragments arranged by size: 3074 2046 552 368 300 79 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 4496 4513 4666 4851 5296 325 Size: 1255 1025 1891 17 153 185 445 1498 Fragments arranged by size: 1891 1498 1255 1025 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2619 3135 Size: 274 80 33 51 1443 105 537 516 Cuts at: 3135 3282 4715 6427 96 Size: 147 1433 1712 138 Fragments arranged by size: 1712 1443 1433 537 516 274 147 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2906 2938 3197 3229 3628 3660 6429 6461 2906 Size: 32 259 32 399 32 2769 32 2914 Fragments arranged by size: 2914 2769 399 259 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3368 1316 Size: 2052 4417 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2719 2885 2698 Size: 21 166 6282 Fragments arranged by size: 6282 166 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6469 XmaI C'CCGG_G Cuts at: 1870 3324 3794 1870 Size: 1454 470 4545 Fragments arranged by size: 4545 1454 470 XmnI GAAnn'nnTTC Cuts at: 2568 2648 2660 4598 2568 Size: 80 12 1938 4439 Fragments arranged by size: 4439 1938 80 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI