Restriction map for
pFN21AE3910
(Linear) (Six-base) MAPSORT of: KIEE3910.seq Check: 2485 from: 1 to: 1764
KIEE3910.orf
With 182 enzymes: SgfI *
March 22, 2012 16:49 ..
AccI GT'mk_AC
Cuts at: 0 809 1764
Size: 809 955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1764
Size: 1045 719
AleI CACnn'nnGTG
Cuts at: 0 1108 1249 1507 1764
Size: 1108 141 258 257
Fragments arranged by size:
1108 258 257 141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1764
Size: 724 32 1008
Fragments arranged by size:
1008 724 32
AlwNI CAG_nnn'CTG
Cuts at: 0 51 255 1513 1764
Size: 51 204 1258 251
Fragments arranged by size:
1258 251 204 51
ApaI G_GGCC'C
Cuts at: 0 828 1764
Size: 828 936
ApaLI G'TGCA_C
Cuts at: 0 1441 1764
Size: 1441 323
ApoI r'AATT_y
Cuts at: 0 10 1764
Size: 10 1754
AvaI C'yCGr_G
Cuts at: 0 1319 1764
Size: 1319 445
BanI G'GyrC_C
Cuts at: 0 1000 1208 1243 1764
Size: 1000 208 35 521
Fragments arranged by size:
1000 521 208 35
BanII G_rGCy'C
Cuts at: 0 235 324 434 505 828 929 1764
Size: 235 89 110 71 323 101 835
Fragments arranged by size:
835 323 235 110 101 89 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 193 587 1033 1089 1687 1764
Size: 105 88 394 446 56 598 77
Fragments arranged by size:
598 446 394 105 88 77 56
BclI T'GATC_A
Cuts at: 0 1253 1764
Size: 1253 511
BglI GCCn_nnn'nGGC
Cuts at: 0 1089 1764
Size: 1089 675
BlpI GC'TnA_GC
Cuts at: 0 419 592 1764
Size: 419 173 1172
Fragments arranged by size:
1172 419 173
Bme1580I G_kGCm'C
Cuts at: 0 828 1445 1764
Size: 828 617 319
Fragments arranged by size:
828 617 319
BmgBI CAC'GTC
Cuts at: 0 111 1362 1764
Size: 111 1251 402
Fragments arranged by size:
1251 402 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 495 1364 1764
Size: 90 405 869 400
Fragments arranged by size:
869 405 400 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 753 1764
Size: 753 1011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 1764
Size: 156 1608
Bpu10I CC'TnA_GC
Cuts at: 0 94 229 1764
Size: 94 135 1535
Fragments arranged by size:
1535 135 94
BsaAI yAC'GTr
Cuts at: 0 570 1764
Size: 570 1194
BsaBI GATnn'nnATC
Cuts at: 0 1228 1764
Size: 1228 536
BsaWI w'CCGG_w
Cuts at: 0 620 1764
Size: 620 1144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 901 931 1674 1704 1764
Size: 901 30 743 30 60
Fragments arranged by size:
901 743 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 174 319 1682 1764
Size: 174 145 1363 82
Fragments arranged by size:
1363 174 145 82
BsiHKAI G_wGCw'C
Cuts at: 0 434 575 929 1445 1764
Size: 434 141 354 516 319
Fragments arranged by size:
516 434 354 319 141
BsmI GAATG_Cn'
Cuts at: 0 464 1764
Size: 464 1300
Bsp1286I G_dGCh'C
Cuts at: 0 235 324 434 505 575 828 929 1445
Size: 235 89 110 71 70 253 101 516
Cuts at: 1445 1764
Size: 319
Fragments arranged by size:
516 319 253 235 110 101 89 71
70
BspEI T'CCGG_A
Cuts at: 0 620 1764
Size: 620 1144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1088 1764
Size: 1088 676
BsrBI CCG'CTC
Cuts at: 0 789 1194 1764
Size: 789 405 570
Fragments arranged by size:
789 570 405
BsrDI GCAATG_nn'
Cuts at: 0 135 183 1148 1764
Size: 135 48 965 616
Fragments arranged by size:
965 616 135 48
BsrFI r'CCGG_y
Cuts at: 0 1451 1764
Size: 1451 313
BsrGI T'GTAC_A
Cuts at: 0 1277 1764
Size: 1277 487
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 961 1764
Size: 516 445 803
Fragments arranged by size:
803 516 445
BstEII G'GTnAC_C
Cuts at: 0 1110 1764
Size: 1110 654
Bsu36I CC'TnA_GG
Cuts at: 0 497 1764
Size: 497 1267
BtgI C'CryG_G
Cuts at: 0 839 867 1106 1235 1764
Size: 839 28 239 129 529
Fragments arranged by size:
839 529 239 129 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 109 1625 1764
Size: 109 1516 139
Fragments arranged by size:
1516 139 109
BtsI GCAGTG_nn'
Cuts at: 0 1601 1764
Size: 1601 163
DraIII CAC_nnn'GTG
Cuts at: 0 961 1764
Size: 961 803
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 1764
Size: 109 1655
EaeI y'GGCC_r
Cuts at: 0 540 790 855 870 1764
Size: 540 250 65 15 894
Fragments arranged by size:
894 540 250 65 15
EarI CTCTTCn'nnn_
Cuts at: 0 415 1764
Size: 415 1349
EcoICRI GAG'CTC
Cuts at: 0 432 927 1764
Size: 432 495 837
Fragments arranged by size:
837 495 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 1045 1764
Size: 156 889 719
Fragments arranged by size:
889 719 156
EcoO109I rG'GnC_Cy
Cuts at: 0 824 825 1764
Size: 824 1 939
Fragments arranged by size:
939 824 1
EcoRI G'AATT_C
Cuts at: 0 10 1764
Size: 10 1754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 672 1764
Size: 672 1092
HaeII r_GCGC'y
Cuts at: 0 592 1764
Size: 592 1172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1674 1706 1764
Size: 1042 32 600 32 58
Fragments arranged by size:
1042 600 58 32 32
MfeI C'AATT_G
Cuts at: 0 606 1764
Size: 606 1158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1443 1764
Size: 1196 247 321
Fragments arranged by size:
1196 321 247
MscI TGG'CCA
Cuts at: 0 857 1764
Size: 857 907
MslI CAynn'nnrTG
Cuts at: 0 128 1108 1234 1249 1507 1764
Size: 128 980 126 15 258 257
Fragments arranged by size:
980 258 257 128 126 15
MspA1I CmG'CkG
Cuts at: 0 193 816 1529 1764
Size: 193 623 713 235
Fragments arranged by size:
713 623 235 193
NcoI C'CATG_G
Cuts at: 0 1106 1235 1764
Size: 1106 129 529
Fragments arranged by size:
1106 529 129
NspI r_CATG'y
Cuts at: 0 939 1764
Size: 939 825
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 1107 1764
Size: 255 852 657
Fragments arranged by size:
852 657 255
PfoI T'CCnGG_A
Cuts at: 0 905 1060 1764
Size: 905 155 704
Fragments arranged by size:
905 704 155
PmlI CAC'GTG
Cuts at: 0 570 1764
Size: 570 1194
PspOMI G'GGCC_C
Cuts at: 0 824 1764
Size: 824 940
PstI C_TGCA'G
Cuts at: 0 47 1478 1764
Size: 47 1431 286
Fragments arranged by size:
1431 286 47
PvuII CAG'CTG
Cuts at: 0 1529 1764
Size: 1529 235
SacI G_AGCT'C
Cuts at: 0 434 929 1764
Size: 434 495 835
Fragments arranged by size:
835 495 434
ScaI AGT'ACT
Cuts at: 0 79 1764
Size: 79 1685
SfcI C'TryA_G
Cuts at: 0 43 267 1474 1764
Size: 43 224 1207 290
Fragments arranged by size:
1207 290 224 43
SmaI CCC'GGG
Cuts at: 0 1321 1764
Size: 1321 443
StyI C'CwwG_G
Cuts at: 0 1106 1156 1235 1535 1764
Size: 1106 50 79 300 229
Fragments arranged by size:
1106 300 229 79 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 1764
Size: 600 1164
TatI w'GTAC_w
Cuts at: 0 77 614 1130 1277 1764
Size: 77 537 516 147 487
Fragments arranged by size:
537 516 487 147 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 901 933 1192 1224 1623 1655 1764
Size: 901 32 259 32 399 32 109
Fragments arranged by size:
901 399 259 109 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1363 1764
Size: 1363 401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 714 880 1764
Size: 693 21 166 884
Fragments arranged by size:
884 693 166 21
XmaI C'CCGG_G
Cuts at: 0 1319 1764
Size: 1319 445
XmnI GAAnn'nnTTC
Cuts at: 0 563 643 655 1764
Size: 563 80 12 1109
Fragments arranged by size:
1109 563 80 12
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaWI
BsaXI BseRI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstEII Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I MfeI MmeI MscI MslI
MspA1I NcoI NspI PflMI PfoI PmlI PspOMI PstI
PvuII SacI ScaI SfcI SmaI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglII BmtI BpuEI
BsaI BsaHI BseYI BsgI BsiEI BsiWI BsmBI BspHI
BssHII BssSI BstBI BstXI BstYI BstZ17I ClaI CspCI
DraI EagI EciI EcoNI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI ZraI