Restriction map for
pFN21AE6401
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 427 GTCTAC GT^[AC][GT]AC
AflIII 356 ACGTGT A^C[GA][CT]GT
AocI 1302 CCTCAGG CC^T[ACGT]AGG
AspHI 306 GTGCTC G[AT]GC[AT]^C
BalI 1058 TGGCCA TGG^CCA
BbrPI 1054 CACGTG CAC^GTG
BglII 1196 AGATCT A^GATCT
BpmI 535 CTGGAG CTGGAG(16/14)
BsaAI 1054 CACGTG [CT]AC^GT[GA]
BsiHKAI 306 GTGCTC G[AT]GC[AT]^C
BsmBI 780 CGTCTC CGTCTC(1/5)
BsmFI 1375 GTCCC GGGAC(10/14)
BsrI 1250 ACTGG ACTGG(1/-1)
BstAPI 1127 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1196 AGATCT [GA]^GATC[CT]
Bsu36I 1302 CCTCAGG CC^T[ACGT]AGG
DpnII 1197 GATC ^GATC
EagI 66 CGGCCG C^GGCCG
EclXI 66 CGGCCG C^GGCCG
Eco52I 66 CGGCCG C^GGCCG
Eco81I 1302 CCTCAGG CC^T[ACGT]AGG
EcoNI 1290 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 814 GTCAAC GT[CT]^[GA]AC
HindII 814 GTCAAC GT[CT]^[GA]AC
MboI 1197 GATC ^GATC
MflI 1196 AGATCT [GA]^GATC[CT]
MluNI 1058 TGGCCA TGG^CCA
MscI 1058 TGGCCA TGG^CCA
NcoI 653 CCATGG C^CATGG
NdeII 1197 GATC ^GATC
NotI 65 GCGGCCGC GC^GGCCGC
PflMI 1172 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1054 CACGTG CAC^GTG
PmlI 1054 CACGTG CAC^GTG
PstI 160 CTGCAG CTGCA^G
Sau3AI 1197 GATC ^GATC
SfiI 1110 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TspRI 1350 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1172 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1196 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 799 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
904 CAGGTGCTG
ApaI 105 GGGCCC GGGCC^C
1168 GGGCCC
AvaI 7 CCCGAG C^[CT]CG[GA]G
607 CCCGAG
BbsI 697 GAAGAC GAAGAC(2/6)
76 GTCTTC
BfaI 155 CTAG C^TAG
415 CTAG
BlpI 291 GCTGAGC GC^T[ACGT]AGC
715 GCTGAGC
Bpu1102I 291 GCTGAGC GC^T[ACGT]AGC
715 GCTGAGC
BpuAI 697 GAAGAC GAAGAC(2/6)
76 GTCTTC
BsaI 610 GAGACC GGTCTC(1/5)
838 GAGACC
BsgI 1079 CTGCAC GTGCAG(16/14)
481 GTGCAG
BsiEI 66 CGGCCG CG[GA][CT]^CG
884 CGGTCG
BsoBI 7 CCCGAG C^[CT]CG[GA]G
607 CCCGAG
BssHII 56 GCGCGC G^CGCGC
165 GCGCGC
BssSI 996 CACGAG CACGAG(-5/-1)
1267 CTCGTG
CelII 291 GCTGAGC GC^T[ACGT]AGC
715 GCTGAGC
EarI 1138 CTCTTC CTCTTC(1/4)
850 GAAGAG
EcoT14I 653 CCATGG C^C[AT][AT]GG
1274 CCAAGG
HinfI 630 GACTC G^A[ACGT]TC
1395 GACTC
Ksp632I 1138 CTCTTC CTCTTC(1/4)
850 GAAGAG
MaeI 155 CTAG C^TAG
415 CTAG
MaeII 356 ACGT A^CGT
1055 ACGT
MaeIII 226 GTGAC ^GT[ACGT]AC
1270 GTGAC
MslI 900 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
915 CACAGCTGTG
NaeI 325 GCCGGC GCC^GGC
1147 GCCGGC
NgoMIV 325 GCCGGC G^CCGGC
1147 GCCGGC
NspI 1130 GCATGT [GA]CATG^[CT]
1209 GCATGT
PleI 630 GACTC GAGTC(4/5)
1395 GACTC
PpuMI 11 AGGACCC [GA]G^G[AT]CC[CT]
1298 AGGTCCT
PvuII 802 CAGCTG CAG^CTG
917 CAGCTG
SfcI 135 CTACAG C^T[GA][CT]AG
160 CTGCAG
StyI 653 CCATGG C^C[AT][AT]GG
1274 CCAAGG
Tsp45I 226 GTGAC ^GT[GC]AC
1270 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI KspI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
SacII Sau96I ScrFI SfaNI SfoI
TseI XcmI