(Circular) (Six-base) MAPSORT of: pFN21AE6401.seq Check: 8004 from: 1 to: 6136 pFN21AE6401 6136 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2433 3479 1937 Size: 496 1046 4594 Fragments arranged by size: 4594 1046 496 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3337 4199 5583 5851 1632 Size: 1705 862 1384 268 1917 Fragments arranged by size: 1917 1705 1384 862 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 2361 5035 6012 1203 Size: 1158 2674 977 1327 Fragments arranged by size: 2674 1327 1158 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 2924 1166 Size: 1758 4378 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AlwNI CAG_nnn'CTG Cuts at: 2809 2914 3879 5451 2809 Size: 105 965 1572 3494 Fragments arranged by size: 3494 1572 965 105 ApaI G_GGCC'C Cuts at: 2114 3177 2114 Size: 1063 5073 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 3445 3671 3823 5932 1493 Size: 1952 226 152 2109 1697 Fragments arranged by size: 2109 1952 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2612 3461 1870 Size: 73 69 600 849 4545 Fragments arranged by size: 4545 849 600 73 69 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 2646 3014 3073 3457 Size: 325 217 627 453 406 368 59 384 Cuts at: 3457 4912 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 453 406 384 368 325 217 59 BanII G_rGCy'C Cuts at: 1391 2114 2687 3177 3455 1391 Size: 723 573 490 278 4072 Fragments arranged by size: 4072 723 573 490 278 BbeI G_GCGC'C Cuts at: 2650 3018 3077 2650 Size: 368 59 5709 Fragments arranged by size: 5709 368 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2074 2709 928 Size: 971 175 635 4355 Fragments arranged by size: 4355 971 635 175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4327 4361 1903 Size: 34 2390 34 3678 Fragments arranged by size: 3678 2390 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 1548 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2525 3071 3122 Size: 107 122 71 631 605 853 546 51 Cuts at: 3122 4747 136 Size: 1625 1525 Fragments arranged by size: 1625 1525 853 631 605 546 122 107 71 51 BglII A'GATC_T Cuts at: 3201 6131 3201 Size: 2930 3206 BlpI GC'TnA_GC Cuts at: 2297 2721 3553 2297 Size: 424 832 4880 Fragments arranged by size: 4880 832 424 Bme1580I G_kGCm'C Cuts at: 1790 2114 2322 2577 3177 4197 5353 1790 Size: 324 208 255 600 1020 1156 2573 Fragments arranged by size: 2573 1156 1020 600 324 255 208 BmgBI CAC'GTC Cuts at: 1318 2362 1318 Size: 1044 5092 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3867 4825 442 Size: 416 53 83 399 24 2450 958 1753 Fragments arranged by size: 2450 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 4796 1239 Size: 1322 2235 2579 Fragments arranged by size: 2579 2235 1322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4261 5126 5424 4261 Size: 865 298 4973 Fragments arranged by size: 4973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2609 2837 4799 882 Size: 617 1110 228 1962 2219 Fragments arranged by size: 2219 1962 1110 617 228 BsaAI yAC'GTr Cuts at: 493 1204 3061 3927 493 Size: 711 1857 866 2702 Fragments arranged by size: 2702 1857 866 711 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2202 2647 2784 Size: 53 83 186 936 476 193 445 137 Cuts at: 2784 3015 3074 3943 4325 4960 275 Size: 231 59 869 382 635 1451 Fragments arranged by size: 1451 936 869 635 476 445 382 231 193 186 137 83 59 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5911 Fragments arranged by size: 5911 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 3043 3179 1186 Size: 1192 665 136 4143 Fragments arranged by size: 4143 1192 665 136 BseYI C'CCAG_C Cuts at: 2031 2260 2374 2455 2532 2563 2697 3168 5339 Size: 229 114 81 77 31 134 471 2171 Cuts at: 5339 5835 2031 Size: 496 2332 Fragments arranged by size: 2332 2171 496 471 229 134 114 81 77 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 3069 1163 Size: 573 771 562 4230 Fragments arranged by size: 4230 771 573 562 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2074 2892 3586 4347 5375 1540 Size: 275 186 73 818 694 761 1028 2301 Fragments arranged by size: 2301 1028 818 761 694 275 186 73 BsiHKAI G_wGCw'C Cuts at: 2315 4197 4282 5353 2315 Size: 1882 85 1071 3098 Fragments arranged by size: 3098 1882 1071 85 BsmI GAATG_Cn' Cuts at: 3647 3740 3647 Size: 93 6043 BsmBI CGTCTCn'nnnn_ Cuts at: 2791 2791 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2315 2322 2577 2687 3177 3455 Size: 399 324 201 7 255 110 490 278 Cuts at: 3455 4197 4282 5353 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 324 278 255 201 110 85 7 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4023 1394 Size: 429 2200 3507 Fragments arranged by size: 3507 2200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2330 2393 3152 4780 4967 1700 Size: 110 520 63 759 1628 187 2869 Fragments arranged by size: 2869 1628 759 520 187 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 2061 2170 1925 Size: 136 109 5891 Fragments arranged by size: 5891 136 109 BssSI C'ACGA_G Cuts at: 3001 3272 4196 5208 3001 Size: 271 924 1012 3929 Fragments arranged by size: 3929 1012 924 271 BstAPI GCAn_nnn'nTGC Cuts at: 794 3138 794 Size: 2344 3792 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstYI r'GATC_y Cuts at: 1972 3201 3466 3833 4218 4235 5687 5773 5785 Size: 1229 265 367 385 17 1452 86 12 Cuts at: 5785 6131 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1229 385 367 346 265 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3308 3308 Size: 6136 BtgI C'CryG_G Cuts at: 513 1065 2068 2244 2349 2658 3126 513 Size: 552 1003 176 105 309 468 3523 Fragments arranged by size: 3523 1003 552 468 309 176 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3133 487 Size: 683 56 1907 3490 Fragments arranged by size: 3490 1907 683 56 BtsI GCAGTG_nn' Cuts at: 3652 4446 4466 3652 Size: 794 20 5322 Fragments arranged by size: 5322 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2151 2328 Size: 54 1425 50 275 18 241 80 177 Cuts at: 2328 2661 3063 3123 3583 4472 6101 8 Size: 333 402 60 460 889 1629 43 Fragments arranged by size: 1629 1425 889 460 402 333 275 241 177 80 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2071 3583 1537 Size: 275 259 1512 4090 Fragments arranged by size: 4090 1512 275 259 EarI CTCTTCn'nnn_ Cuts at: 1353 2850 3149 4066 1353 Size: 1497 299 917 3423 Fragments arranged by size: 3423 1497 917 299 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2971 4723 5097 5243 387 Size: 1064 138 1382 1752 374 146 1280 Fragments arranged by size: 1752 1382 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2561 3337 4199 4796 5583 5851 1239 Size: 393 929 776 862 597 787 268 1524 Fragments arranged by size: 1524 929 862 787 776 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3299 3299 Size: 6136 EcoO109I rG'GnC_Cy Cuts at: 2017 2045 3173 3304 3312 2017 Size: 28 1128 131 8 4841 Fragments arranged by size: 4841 1128 131 28 8 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 2551 2650 3018 3077 5283 1678 Size: 873 99 368 59 2206 2531 Fragments arranged by size: 2531 2206 873 368 99 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3193 3225 4777 4809 4851 Size: 32 135 32 1621 32 1552 32 42 Cuts at: 4851 4883 1373 Size: 32 2626 Fragments arranged by size: 2626 1621 1552 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2821 3480 3726 669 Size: 988 281 883 659 246 3079 Fragments arranged by size: 3079 988 883 659 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KasI G'GCGC_C Cuts at: 2646 3014 3073 2646 Size: 368 59 5709 Fragments arranged by size: 5709 368 59 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5250 5434 1305 Size: 3945 184 2007 Fragments arranged by size: 3945 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3065 10 Size: 54 1425 343 1233 3081 Fragments arranged by size: 3081 1425 1233 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2909 2924 4094 4453 4612 518 Size: 648 1743 15 1170 359 159 2042 Fragments arranged by size: 2042 1743 1170 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2070 2102 2246 2351 2809 2924 3552 4229 Size: 464 32 144 105 458 115 628 677 Cuts at: 4229 5377 5622 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 628 464 458 245 144 115 105 32 NaeI GCC'GGC Cuts at: 2332 3154 2332 Size: 822 5314 NarI GG'CG_CC Cuts at: 2647 3015 3074 2647 Size: 368 59 5709 Fragments arranged by size: 5709 368 59 NcoI C'CATG_G Cuts at: 513 1065 2658 513 Size: 552 1593 3991 Fragments arranged by size: 3991 1593 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NgoMIV G'CCGG_C Cuts at: 2330 3152 2330 Size: 822 5314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 2071 3583 2071 Size: 1512 4624 NspI r_CATG'y Cuts at: 1132 1229 3139 3218 3494 5039 1132 Size: 97 1910 79 276 1545 2229 Fragments arranged by size: 2229 1910 1545 276 97 79 PasI CC'CwG_GG Cuts at: 1348 2107 2236 3034 1348 Size: 759 129 798 4450 Fragments arranged by size: 4450 798 759 129 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 3183 1108 Size: 2075 4061 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PmlI CAC'GTG Cuts at: 3061 3061 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2849 2881 4205 4237 5718 5750 2849 Size: 32 1324 32 1481 32 3235 Fragments arranged by size: 3235 1481 1324 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2017 3304 2017 Size: 1287 4849 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspOMI G'GGCC_C Cuts at: 2110 3173 2110 Size: 1063 5073 PstI C_TGCA'G Cuts at: 830 2169 3488 830 Size: 1339 1319 3478 Fragments arranged by size: 3478 1339 1319 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2809 2924 2809 Size: 115 6021 SacII CC_GC'GG Cuts at: 2071 2247 2352 2071 Size: 176 105 5855 Fragments arranged by size: 5855 176 105 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 2140 2165 3484 4619 5300 5491 826 Size: 219 1095 25 1319 1135 681 191 1471 Fragments arranged by size: 1471 1319 1135 1095 681 219 191 25 SfiI GGCCn_nnn'nGGCC Cuts at: 3122 3122 Size: 6136 SfoI GGC'GCC Cuts at: 2648 3016 3075 2648 Size: 368 59 5709 Fragments arranged by size: 5709 368 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4240 5141 5403 820 Size: 197 926 2297 901 262 1553 Fragments arranged by size: 2297 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2658 3279 3575 513 Size: 552 1593 621 296 3074 Fragments arranged by size: 3074 1593 621 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2228 2524 2878 2888 4163 4180 4333 Size: 1255 648 296 354 10 1275 17 153 Cuts at: 4333 4518 4963 325 Size: 185 445 1498 Fragments arranged by size: 1498 1275 1255 648 445 354 296 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3041 3109 3259 3041 Size: 68 150 5918 Fragments arranged by size: 5918 150 68 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 4265 4265 Size: 6136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI