(Linear) (Six-base) MAPSORT of: KIEE6401.seq Check: 7157 from: 1 to: 1431 KIEE6401.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 428 1431 Size: 428 1003 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1332 1431 Size: 1332 99 AflIII A'CryG_T Cuts at: 0 356 1431 Size: 356 1075 AleI CACnn'nnGTG Cuts at: 0 919 1431 Size: 919 512 AlwNI CAG_nnn'CTG Cuts at: 0 804 909 1431 Size: 804 105 522 Fragments arranged by size: 804 522 105 ApaI G_GGCC'C Cuts at: 0 109 1172 1431 Size: 109 1063 259 Fragments arranged by size: 1063 259 109 AvaI C'yCGr_G Cuts at: 0 7 607 1431 Size: 7 600 824 Fragments arranged by size: 824 600 7 BanI G'GyrC_C Cuts at: 0 235 641 1009 1068 1431 Size: 235 406 368 59 363 Fragments arranged by size: 406 368 363 235 59 BanII G_rGCy'C Cuts at: 0 109 682 1172 1431 Size: 109 573 490 259 Fragments arranged by size: 573 490 259 109 BbeI G_GCGC'C Cuts at: 0 645 1013 1072 1431 Size: 645 368 59 359 Fragments arranged by size: 645 368 359 59 BbsI GAAGACnn'nnnn_ Cuts at: 0 69 704 1431 Size: 69 635 727 Fragments arranged by size: 727 635 69 BglI GCCn_nnn'nGGC Cuts at: 0 520 1066 1117 1431 Size: 520 546 51 314 Fragments arranged by size: 546 520 314 51 BglII A'GATC_T Cuts at: 0 1196 1431 Size: 1196 235 BlpI GC'TnA_GC Cuts at: 0 292 716 1431 Size: 292 424 715 Fragments arranged by size: 715 424 292 Bme1580I G_kGCm'C Cuts at: 0 109 317 572 1172 1431 Size: 109 208 255 600 259 Fragments arranged by size: 600 259 255 208 109 BmgBI CAC'GTC Cuts at: 0 357 1431 Size: 357 1074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1431 Size: 556 875 BsaI GGTCTCn'nnnn_ Cuts at: 0 604 832 1431 Size: 604 228 599 Fragments arranged by size: 604 599 228 BsaAI yAC'GTr Cuts at: 0 1056 1431 Size: 1056 375 BsaHI Gr'CG_yC Cuts at: 0 4 197 642 779 1010 1069 1431 Size: 4 193 445 137 231 59 362 Fragments arranged by size: 445 362 231 193 137 59 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 373 1038 1174 1431 Size: 373 665 136 257 Fragments arranged by size: 665 373 257 136 BseYI C'CCAG_C Cuts at: 0 26 255 369 450 527 558 692 1163 Size: 26 229 114 81 77 31 134 471 Cuts at: 1163 1431 Size: 268 Fragments arranged by size: 471 268 229 134 114 81 77 31 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 1064 1431 Size: 502 562 367 Fragments arranged by size: 562 502 367 BsiEI CG_ry'CG Cuts at: 0 69 887 1431 Size: 69 818 544 Fragments arranged by size: 818 544 69 BsiHKAI G_wGCw'C Cuts at: 0 310 1431 Size: 310 1121 BsmBI CGTCTCn'nnnn_ Cuts at: 0 786 1431 Size: 786 645 Bsp1286I G_dGCh'C Cuts at: 0 109 310 317 572 682 1172 1431 Size: 109 201 7 255 110 490 259 Fragments arranged by size: 490 259 255 201 110 109 7 BsrFI r'CCGG_y Cuts at: 0 325 388 1147 1431 Size: 325 63 759 284 Fragments arranged by size: 759 325 284 63 BssHII G'CGCG_C Cuts at: 0 56 165 1431 Size: 56 109 1266 Fragments arranged by size: 1266 109 56 BssSI C'ACGA_G Cuts at: 0 996 1267 1431 Size: 996 271 164 Fragments arranged by size: 996 271 164 BstAPI GCAn_nnn'nTGC Cuts at: 0 1133 1431 Size: 1133 298 BstYI r'GATC_y Cuts at: 0 1196 1431 Size: 1196 235 Bsu36I CC'TnA_GG Cuts at: 0 1303 1431 Size: 1303 128 BtgI C'CryG_G Cuts at: 0 63 239 344 653 1121 1431 Size: 63 176 105 309 468 310 Fragments arranged by size: 468 310 309 176 105 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1128 1431 Size: 1128 303 EaeI y'GGCC_r Cuts at: 0 66 146 323 656 1058 1118 1431 Size: 66 80 177 333 402 60 313 Fragments arranged by size: 402 333 313 177 80 66 60 EagI C'GGCC_G Cuts at: 0 66 1431 Size: 66 1365 EarI CTCTTCn'nnn_ Cuts at: 0 845 1144 1431 Size: 845 299 287 Fragments arranged by size: 845 299 287 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 966 1431 Size: 966 465 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1332 1431 Size: 556 776 99 Fragments arranged by size: 776 556 99 EcoNI CCTnn'n_nnAGG Cuts at: 0 1294 1431 Size: 1294 137 EcoO109I rG'GnC_Cy Cuts at: 0 12 40 1168 1299 1307 1431 Size: 12 28 1128 131 8 124 Fragments arranged by size: 1128 131 124 28 12 8 HaeII r_GCGC'y Cuts at: 0 546 645 1013 1072 1431 Size: 546 99 368 59 359 Fragments arranged by size: 546 368 359 99 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1188 1220 1431 Size: 1188 32 211 Fragments arranged by size: 1188 211 32 HincII GTy'rAC Cuts at: 0 816 1431 Size: 816 615 KasI G'GCGC_C Cuts at: 0 641 1009 1068 1431 Size: 641 368 59 363 Fragments arranged by size: 641 368 363 59 MscI TGG'CCA Cuts at: 0 1060 1431 Size: 1060 371 MslI CAynn'nnrTG Cuts at: 0 904 919 1431 Size: 904 15 512 Fragments arranged by size: 904 512 15 MspA1I CmG'CkG Cuts at: 0 65 97 241 346 804 919 1431 Size: 65 32 144 105 458 115 512 Fragments arranged by size: 512 458 144 115 105 65 32 NaeI GCC'GGC Cuts at: 0 327 1149 1431 Size: 327 822 282 Fragments arranged by size: 822 327 282 NarI GG'CG_CC Cuts at: 0 642 1010 1069 1431 Size: 642 368 59 362 Fragments arranged by size: 642 368 362 59 NcoI C'CATG_G Cuts at: 0 653 1431 Size: 653 778 NgoMIV G'CCGG_C Cuts at: 0 325 1147 1431 Size: 325 822 284 Fragments arranged by size: 822 325 284 NotI GC'GGCC_GC Cuts at: 0 66 1431 Size: 66 1365 NspI r_CATG'y Cuts at: 0 1134 1213 1431 Size: 1134 79 218 Fragments arranged by size: 1134 218 79 PasI CC'CwG_GG Cuts at: 0 102 231 1029 1431 Size: 102 129 798 402 Fragments arranged by size: 798 402 129 102 PflMI CCAn_nnn'nTGG Cuts at: 0 1178 1431 Size: 1178 253 PmlI CAC'GTG Cuts at: 0 1056 1431 Size: 1056 375 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 844 876 1431 Size: 844 32 555 Fragments arranged by size: 844 555 32 PpuMI rG'GwC_Cy Cuts at: 0 12 1299 1431 Size: 12 1287 132 Fragments arranged by size: 1287 132 12 PspOMI G'GGCC_C Cuts at: 0 105 1168 1431 Size: 105 1063 263 Fragments arranged by size: 1063 263 105 PstI C_TGCA'G Cuts at: 0 164 1431 Size: 164 1267 PvuII CAG'CTG Cuts at: 0 804 919 1431 Size: 804 115 512 Fragments arranged by size: 804 512 115 SacII CC_GC'GG Cuts at: 0 66 242 347 1431 Size: 66 176 105 1084 Fragments arranged by size: 1084 176 105 66 SfcI C'TryA_G Cuts at: 0 135 160 1431 Size: 135 25 1271 Fragments arranged by size: 1271 135 25 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1117 1431 Size: 1117 314 SfoI GGC'GCC Cuts at: 0 643 1011 1070 1431 Size: 643 368 59 361 Fragments arranged by size: 643 368 361 59 StyI C'CwwG_G Cuts at: 0 653 1274 1431 Size: 653 621 157 Fragments arranged by size: 653 621 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 223 519 873 883 1431 Size: 223 296 354 10 548 Fragments arranged by size: 548 354 296 223 10 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1036 1104 1254 1431 Size: 1036 68 150 177 Fragments arranged by size: 1036 177 150 68 Enzymes that do cut: AccI AcuI AflIII AleI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtgZI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacII SfcI SfiI SfoI StyI TaqII XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI