(Linear) (Six-base) MAPSORT of: KIEE6329.seq Check: 3936 from: 1 to: 1092 KIEE6329.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 1092 Size: 337 755 AfeI AGC'GCT Cuts at: 0 860 1092 Size: 860 232 AflIII A'CryG_T Cuts at: 0 395 1092 Size: 395 697 AhdI GACnn_n'nnGTC Cuts at: 0 134 1092 Size: 134 958 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 487 519 1092 Size: 487 32 573 Fragments arranged by size: 573 487 32 AlwNI CAG_nnn'CTG Cuts at: 0 301 432 1035 1092 Size: 301 131 603 57 Fragments arranged by size: 603 301 131 57 ApaI G_GGCC'C Cuts at: 0 40 1092 Size: 40 1052 BanI G'GyrC_C Cuts at: 0 311 622 1092 Size: 311 311 470 Fragments arranged by size: 470 311 311 BanII G_rGCy'C Cuts at: 0 40 77 174 239 614 743 1092 Size: 40 37 97 65 375 129 349 Fragments arranged by size: 375 349 129 97 65 40 37 BbeI G_GCGC'C Cuts at: 0 315 1092 Size: 315 777 BbsI GAAGACnn'nnnn_ Cuts at: 0 240 1092 Size: 240 852 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 717 751 1092 Size: 717 34 341 Fragments arranged by size: 717 341 34 BglI GCCn_nnn'nGGC Cuts at: 0 601 875 1092 Size: 601 274 217 Fragments arranged by size: 601 274 217 BlpI GC'TnA_GC Cuts at: 0 178 328 1092 Size: 178 150 764 Fragments arranged by size: 764 178 150 Bme1580I G_kGCm'C Cuts at: 0 40 1092 Size: 40 1052 BmrI ACTGGGnnnn_n' Cuts at: 0 883 894 1092 Size: 883 11 198 Fragments arranged by size: 883 198 11 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 1092 Size: 91 1001 BsaAI yAC'GTr Cuts at: 0 398 1092 Size: 398 694 BsaHI Gr'CG_yC Cuts at: 0 312 1092 Size: 312 780 BsaWI w'CCGG_w Cuts at: 0 110 632 1092 Size: 110 522 460 Fragments arranged by size: 522 460 110 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 487 517 1092 Size: 487 30 575 Fragments arranged by size: 575 487 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 535 934 1092 Size: 37 498 399 158 Fragments arranged by size: 498 399 158 37 BseYI C'CCAG_C Cuts at: 0 88 98 301 450 596 861 1092 Size: 88 10 203 149 146 265 231 Fragments arranged by size: 265 231 203 149 146 88 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 1092 Size: 420 672 BsiHKAI G_wGCw'C Cuts at: 0 77 989 1092 Size: 77 912 103 Fragments arranged by size: 912 103 77 BsmBI CGTCTCn'nnnn_ Cuts at: 0 916 1092 Size: 916 176 Bsp1286I G_dGCh'C Cuts at: 0 40 77 174 239 614 743 989 1092 Size: 40 37 97 65 375 129 246 103 Fragments arranged by size: 375 246 129 103 97 65 40 37 BspEI T'CCGG_A Cuts at: 0 110 632 1092 Size: 110 522 460 Fragments arranged by size: 522 460 110 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 671 1092 Size: 671 421 BsrBI CCG'CTC Cuts at: 0 182 189 1092 Size: 182 7 903 Fragments arranged by size: 903 182 7 BsrDI GCAATG_nn' Cuts at: 0 363 464 1092 Size: 363 101 628 Fragments arranged by size: 628 363 101 BsrFI r'CCGG_y Cuts at: 0 102 600 814 1092 Size: 102 498 214 278 Fragments arranged by size: 498 278 214 102 BstAPI GCAn_nnn'nTGC Cuts at: 0 375 432 1035 1092 Size: 375 57 603 57 Fragments arranged by size: 603 375 57 57 BstYI r'GATC_y Cuts at: 0 203 773 1092 Size: 203 570 319 Fragments arranged by size: 570 319 203 BtgI C'CryG_G Cuts at: 0 626 693 752 1015 1092 Size: 626 67 59 263 77 Fragments arranged by size: 626 263 77 67 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 954 1092 Size: 954 138 BtsI GCAGTG_nn' Cuts at: 0 56 554 1092 Size: 56 498 538 Fragments arranged by size: 538 498 56 ClaI AT'CG_AT Cuts at: 0 680 1092 Size: 680 412 EaeI y'GGCC_r Cuts at: 0 668 755 1092 Size: 668 87 337 Fragments arranged by size: 668 337 87 EarI CTCTTCn'nnn_ Cuts at: 0 55 1001 1087 1092 Size: 55 946 86 5 Fragments arranged by size: 946 86 55 5 EcoICRI GAG'CTC Cuts at: 0 75 1092 Size: 75 1017 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 337 1092 Size: 91 246 755 Fragments arranged by size: 755 246 91 EcoO109I rG'GnC_Cy Cuts at: 0 305 537 867 936 1011 1092 Size: 305 232 330 69 75 81 Fragments arranged by size: 330 305 232 81 75 69 HaeII r_GCGC'y Cuts at: 0 315 407 862 881 1023 1092 Size: 315 92 455 19 142 69 Fragments arranged by size: 455 315 142 92 69 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 120 152 1092 Size: 120 32 940 Fragments arranged by size: 940 120 32 KasI G'GCGC_C Cuts at: 0 311 1092 Size: 311 781 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 473 1092 Size: 473 619 MscI TGG'CCA Cuts at: 0 670 757 1092 Size: 670 87 335 Fragments arranged by size: 670 335 87 MspA1I CmG'CkG Cuts at: 0 324 450 650 1035 1092 Size: 324 126 200 385 57 Fragments arranged by size: 385 324 200 126 57 NaeI GCC'GGC Cuts at: 0 104 602 816 1092 Size: 104 498 214 276 Fragments arranged by size: 498 276 214 104 NarI GG'CG_CC Cuts at: 0 312 1092 Size: 312 780 NcoI C'CATG_G Cuts at: 0 752 1092 Size: 752 340 NgoMIV G'CCGG_C Cuts at: 0 102 600 814 1092 Size: 102 498 214 278 Fragments arranged by size: 498 278 214 102 PasI CC'CwG_GG Cuts at: 0 339 1092 Size: 339 753 PflMI CCAn_nnn'nTGG Cuts at: 0 591 699 786 1092 Size: 591 108 87 306 Fragments arranged by size: 591 306 108 87 PfoI T'CCnGG_A Cuts at: 0 506 1092 Size: 506 586 PmlI CAC'GTG Cuts at: 0 398 1092 Size: 398 694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 487 519 1092 Size: 487 32 573 Fragments arranged by size: 573 487 32 PpuMI rG'GwC_Cy Cuts at: 0 936 1011 1092 Size: 936 75 81 Fragments arranged by size: 936 81 75 PspOMI G'GGCC_C Cuts at: 0 36 1092 Size: 36 1056 PstI C_TGCA'G Cuts at: 0 437 609 1031 1092 Size: 437 172 422 61 Fragments arranged by size: 437 422 172 61 PvuII CAG'CTG Cuts at: 0 324 450 1035 1092 Size: 324 126 585 57 Fragments arranged by size: 585 324 126 57 SacI G_AGCT'C Cuts at: 0 77 1092 Size: 77 1015 SapI GCTCTTCn'nnn_ Cuts at: 0 1087 1092 Size: 1087 5 SfcI C'TryA_G Cuts at: 0 433 605 1027 1092 Size: 433 172 422 65 Fragments arranged by size: 433 422 172 65 SfoI GGC'GCC Cuts at: 0 313 1092 Size: 313 779 StyI C'CwwG_G Cuts at: 0 40 752 908 974 1092 Size: 40 712 156 66 118 Fragments arranged by size: 712 156 118 66 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 706 839 1092 Size: 706 133 253 Fragments arranged by size: 706 253 133 XmnI GAAnn'nnTTC Cuts at: 0 502 1092 Size: 502 590 Enzymes that do cut: AcuI AfeI AflIII AhdI AloI AlwNI ApaI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstYI BtgI BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfoI StyI TaqII XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BsmI BspHI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI