##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     CelII       CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MwoI        NarI        NcoI        NdeI        NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII           5  CGCG                 CG^CG
AciI           35  GCGG                 CCGC(-3/-1)
AfaI           27  GTAC                 GT^AC
AlwI            8  GATCC                GGATC(4/5)
AocI          100  CCTGAGG              CC^T[ACGT]AGG
ApaI          174  GGGCCC               GGGCC^C
Asp700I       237  GAAAGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI         685  GCTGAGG              CCTCAGC(-5/-2)
BpmI          484  CTGGAG               CTGGAG(16/14)
BsmAI         160  GAGAC                GTCTC(1/5)
BsmBI         160  GAGACG               CGTCTC(1/5)
BspHI         708  TCATGA               T^CATGA
BsrI           74  ACTGG                ACTGG(1/-1)
BssSI         157  CACGAG               CACGAG(-5/-1)
BstF5I        654  GGATG                GGATG(2/0)
BstUI           5  CGCG                 CG^CG
Bsu36I        100  CCTGAGG              CC^T[ACGT]AGG
Eco81I        100  CCTGAGG              CC^T[ACGT]AGG
FauI           35  GCGGG                CCCGC(4/6)
FokI          654  GGATG                GGATG(9/13)
FseI           77  GGCCGGCC             GGCCGG^CC
MaeII         501  ACGT                 A^CGT
MaeIII        362  GTGAC                ^GT[ACGT]AC
MvnI            5  CGCG                 CG^CG
NaeI           78  GCCGGC               GCC^GGC
NgoMIV         78  GCCGGC               G^CCGGC
NlaIII        709  CATG                 CATG^
NruI            4  TCGCGA               TCG^CGA
PleI          490  GACTC                GAGTC(4/5)
PstI          353  CTGCAG               CTGCA^G
RcaI          708  TCATGA               T^CATGA
RsaI           27  GTAC                 GT^AC
SapI          328  GAAGAGC              GCTCTTC(1/4)
SexAI          41  ACCTGGT              A^CC[AT]GGT
SfcI          353  CTGCAG               C^T[GA][CT]AG
TaqI          266  TCGA                 T^CGA
TfiI          202  GAATC                G^A[AT]TC
Tsp45I        362  GTGAC                ^GT[GC]AC
TspRI          73  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       266  TCGA                 T^CGA
XmnI          237  GAAAGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AvaII          39  GGACC                G^G[AT]CC
              152  GGACC               
BsmI          381  GAATGC               GAATGC(1/-1)
              166  GCATTC              
BsrFI          14  ACCGGC               [GA]^CCGG[CT]
               78  GCCGGC              
Cfr10I         14  ACCGGC               [GA]^CCGG[CT]
               78  GCCGGC              
EaeI           76  TGGCCG               [CT]^GGCC[GA]
              618  TGGCCG              
HinfI         202  GAATC                G^A[ACGT]TC
              490  GACTC               
HphI          361  GGTGA                GGTGA(8/7)
              367  GGTGA               
PpuMI          38  GGGACCT              [GA]G^G[AT]CC[CT]
              151  GGGACCC             


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AluI        BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HpaII       ItaI        Ksp632I     MboI        MboII       
MnlI        MspI        MvaI        NciI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TseI