################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI AspEI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KspI MfeI MluI MroI MseI MunI NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 159 CGCG CG^CG AclI 1493 AACGTT AA^CGTT AflIII 596 ACGTGT A^C[GA][CT]GT ApaI 1238 GGGCCC GGGCC^C AspI 1081 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 780 TGGCCA TGG^CCA BbsI 931 GTCTTC GAAGAC(2/6) BfaI 551 CTAG C^TAG BpuAI 931 GTCTTC GAAGAC(2/6) BsaAI 1303 TACGTA [CT]AC^GT[GA] BsaBI 940 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 155 CGGCCG CG[GA][CT]^CG BsmBI 1086 CGTCTC CGTCTC(1/5) BsrFI 1228 GCCGGC [GA]^CCGG[CT] BstEII 1218 GGTCACC G^GT[ACGT]ACC BstPI 1218 GGTCACC G^GT[ACGT]ACC BstUI 159 CGCG CG^CG BstYI 1001 AGATCC [GA]^GATC[CT] Cfr10I 1228 GCCGGC [GA]^CCGG[CT] EagI 155 CGGCCG C^GGCCG EarI 1089 CTCTTC CTCTTC(1/4) EclXI 155 CGGCCG C^GGCCG Eco52I 155 CGGCCG C^GGCCG EcoNI 180 CCTCGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1218 GGTCACC G^GT[ACGT]ACC EcoT14I 1223 CCAAGG C^C[AT][AT]GG FauI 494 CCCGC CCCGC(4/6) HgaI 1071 GACGC GACGC(5/10) HincII 1464 GTTGAC GT[CT]^[GA]AC HindII 1464 GTTGAC GT[CT]^[GA]AC Ksp632I 1089 CTCTTC CTCTTC(1/4) MaeI 551 CTAG C^TAG MamI 940 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1001 AGATCC [GA]^GATC[CT] MluNI 780 TGGCCA TGG^CCA MscI 780 TGGCCA TGG^CCA MvnI 159 CGCG CG^CG NaeI 1228 GCCGGC GCC^GGC NdeI 635 CATATG CA^TATG NgoMIV 1228 GCCGGC G^CCGGC PflFI 1081 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 673 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 1493 AACGTT AA^CGTT PvuII 1048 CAGCTG CAG^CTG SfcI 293 CTGTAG C^T[GA][CT]AG SnaBI 1303 TACGTA TAC^GTA StuI 188 AGGCCT AGG^CCT StyI 1223 CCAAGG C^C[AT][AT]GG TaqI 182 TCGA T^CGA Tth111I 1081 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 182 TCGA T^CGA XhoII 1001 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1259 GGTACC G^GTACC 1330 GGTACC AlwI 622 GATCC GGATC(4/5) 1002 GATCC Aor51HI 104 AGCGCT AGC^GCT 197 AGCGCT Asp718I 1259 GGTACC G^GTACC 1330 GGTACC BbvCI 448 GCTGAGG CCTCAGC(-5/-2) 1521 GCTGAGG BseRI 379 CTCCTC GAGGAG(10/8) 959 GAGGAG BsmFI 798 GGGAC GGGAC(10/14) 1408 GTCCC BspHI 662 TCATGA T^CATGA 899 TCATGA DpnII 622 GATC ^GATC 1002 GATC DraII 673 GGGTCCT [GA]G^G[ACGT]CC[CT] 1238 GGGCCCT EaeI 155 CGGCCG [CT]^GGCC[GA] 780 TGGCCA Eco47III 104 AGCGCT AGC^GCT 197 AGCGCT EcoO109I 673 GGGTCCT [GA]G^G[ACGT]CC[CT] 1238 GGGCCCT HaeII 104 AGCGCT [GA]GCGC^[CT] 197 AGCGCT KpnI 1259 GGTACC GGTAC^C 1330 GGTACC MboI 622 GATC ^GATC 1002 GATC MslI 302 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1164 CATACAGGTG MspA1I 1048 CAGCTG C[AC]G^C[GT]G 1509 CCGCTG MwoI 40 GCAGCGTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 49 GCAGGACGGGC NdeII 622 GATC ^GATC 1002 GATC PflMI 11 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1412 CCAGGCTCTGG RcaI 662 TCATGA T^CATGA 899 TCATGA SacI 401 GAGCTC GAGCT^C 658 GAGCTC Sau3AI 622 GATC ^GATC 1002 GATC SexAI 801 ACCAGGT A^CC[AT]GGT 1403 ACCTGGT SfaNI 554 GCATC GCATC(5/9) 609 GCATC TfiI 870 GAATC G^A[AT]TC 1146 GATTC Van91I 11 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1412 CCAGGCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwNI Asp700I AspHI AvaII BanI BanII BbvI BciVI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI XmnI