(Circular) (Six-base) MAPSORT of: pFN21AE4505.seq Check: 2257 from: 1 to: 6292 pFN21AE4505 6292 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2993 2993 Size: 6292 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 1937 Size: 1698 4594 Acc65I G'GTAC_C Cuts at: 3264 3335 3613 3264 Size: 71 278 5943 Fragments arranged by size: 5943 278 71 AclI AA'CG_TT Cuts at: 1563 3499 4419 4792 1563 Size: 1936 920 373 3063 Fragments arranged by size: 3063 1936 920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4355 5739 6007 1632 Size: 2723 1384 268 1917 Fragments arranged by size: 2723 1917 1384 268 AfeI AGC'GCT Cuts at: 2111 2204 2111 Size: 93 6199 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 2601 5191 6168 1203 Size: 1398 2590 977 1327 Fragments arranged by size: 2590 1398 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6292 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3263 3295 3263 Size: 32 6260 AlwNI CAG_nnn'CTG Cuts at: 3079 3423 3440 4035 5607 3079 Size: 344 17 595 1572 3764 Fragments arranged by size: 3764 1572 595 344 17 ApaI G_GGCC'C Cuts at: 3247 3247 Size: 6292 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 3601 3827 3979 6088 1493 Size: 2108 226 152 2109 1697 Fragments arranged by size: 2109 2108 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3617 1870 Size: 73 1674 4545 Fragments arranged by size: 4545 1674 73 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2858 3264 3335 3613 5068 Size: 325 217 627 1071 406 71 278 1455 Cuts at: 5068 618 Size: 1842 Fragments arranged by size: 1842 1455 1071 627 406 325 278 217 71 BanII G_rGCy'C Cuts at: 1391 2410 2667 2853 3247 3611 1391 Size: 1019 257 186 394 364 4072 Fragments arranged by size: 4072 1019 394 364 257 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2929 928 Size: 971 1030 4291 Fragments arranged by size: 4291 1030 971 BbvCI CC'TCA_GC Cuts at: 2454 3527 2454 Size: 1073 5219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2521 2765 2822 4184 5394 2521 Size: 244 57 1362 1210 3419 Fragments arranged by size: 3419 1362 1210 244 57 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 2462 3247 4353 5509 1790 Size: 672 785 1106 1156 2573 Fragments arranged by size: 2573 1156 1106 785 672 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 4023 4981 Size: 416 53 83 399 24 1457 1149 958 Cuts at: 4981 442 Size: 1753 Fragments arranged by size: 1753 1457 1149 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2441 3077 4952 1239 Size: 1055 147 636 1875 2579 Fragments arranged by size: 2579 1875 1055 636 147 Bpu10I CC'TnA_GC Cuts at: 2454 3342 3527 2454 Size: 888 185 5219 Fragments arranged by size: 5219 888 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4417 5282 5580 4417 Size: 865 298 5129 Fragments arranged by size: 5129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4955 882 Size: 617 3456 2219 Fragments arranged by size: 3456 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3310 4083 493 Size: 711 2106 773 2702 Fragments arranged by size: 2702 2106 773 711 BsaBI GATnn'nnATC Cuts at: 2949 6286 2949 Size: 3337 2955 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4099 4481 5116 275 Size: 53 83 186 936 2566 382 635 1451 Fragments arranged by size: 2566 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2958 2988 3378 3408 1178 Size: 30 165 30 1555 30 390 30 4062 Fragments arranged by size: 4062 1555 390 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2979 1186 Size: 1189 604 4499 Fragments arranged by size: 4499 1189 604 BseYI C'CCAG_C Cuts at: 5495 5991 5495 Size: 496 5796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5719 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2163 3742 4503 5531 1540 Size: 275 186 162 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 162 BsiHKAI G_wGCw'C Cuts at: 2410 2667 2912 4353 4438 5509 2410 Size: 257 245 1441 85 1071 3193 Fragments arranged by size: 3193 1441 1071 257 245 85 BsmI GAATG_Cn' Cuts at: 3803 3896 3803 Size: 93 6199 BsmBI CGTCTCn'nnnn_ Cuts at: 3097 3097 Size: 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2410 2462 2667 2853 2912 3247 3611 Size: 399 620 52 205 186 59 335 364 Cuts at: 3611 4353 4438 5509 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 620 399 364 335 205 186 85 59 52 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 2667 2904 4181 5911 2667 Size: 237 1277 1730 3048 Fragments arranged by size: 3048 1730 1277 237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 2553 2993 3647 844 Size: 1379 330 440 654 3489 Fragments arranged by size: 3489 1379 654 440 330 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4787 4961 65 Size: 1183 3539 174 1396 Fragments arranged by size: 3539 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3233 4936 5123 1700 Size: 110 1423 1703 187 2869 Fragments arranged by size: 2869 1703 1423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 3223 1184 Size: 2039 4253 BstYI r'GATC_y Cuts at: 1972 3006 3622 3989 4374 4391 5843 5929 5941 Size: 1034 616 367 385 17 1452 86 12 Cuts at: 5941 6287 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1034 616 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5740 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 3808 4602 4622 3808 Size: 794 20 5478 Fragments arranged by size: 5478 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2785 3739 Size: 54 1425 50 275 18 330 625 954 Cuts at: 3739 4628 6257 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 954 889 625 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2160 3739 1537 Size: 275 348 1579 4090 Fragments arranged by size: 4090 1579 348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3100 4222 1353 Size: 1747 1122 3423 Fragments arranged by size: 3423 1747 1122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2408 2665 2408 Size: 257 6035 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2441 3077 4355 4952 5739 6007 Size: 393 662 147 636 1278 597 787 268 Cuts at: 6007 1239 Size: 1524 Fragments arranged by size: 1524 1278 787 662 636 597 393 268 147 EcoNI CCTnn'n_nnAGG Cuts at: 2189 2189 Size: 6292 EcoO109I rG'GnC_Cy Cuts at: 2679 3244 2679 Size: 565 5727 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3049 3253 3049 Size: 204 6088 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 2113 2206 5439 1678 Size: 435 93 3233 2531 Fragments arranged by size: 3233 2531 435 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 2796 2828 3077 Size: 32 135 32 569 32 623 32 249 Cuts at: 3077 3109 4933 4965 5007 5039 1373 Size: 32 1824 32 42 32 2626 Fragments arranged by size: 2626 1824 623 569 249 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3636 3882 669 Size: 988 281 1533 165 246 3079 Fragments arranged by size: 3079 1533 988 281 246 165 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3268 3339 3617 3268 Size: 71 278 5943 Fragments arranged by size: 5943 278 71 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 2505 3021 5406 5590 1305 Size: 822 378 516 2385 184 2007 Fragments arranged by size: 2385 2007 822 516 378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2787 10 Size: 54 1425 343 955 3515 Fragments arranged by size: 3515 1425 955 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2311 3173 4250 4609 4768 518 Size: 648 1145 862 1077 359 159 2042 Fragments arranged by size: 2042 1145 1077 862 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3055 3516 3708 4385 5533 5778 1606 Size: 1449 461 192 677 1148 245 2120 Fragments arranged by size: 2120 1449 1148 677 461 245 192 NaeI GCC'GGC Cuts at: 3235 3235 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5740 NdeI CA'TA_TG Cuts at: 387 2641 387 Size: 2254 4038 NgoMIV G'CCGG_C Cuts at: 3233 3233 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3650 5195 1132 Size: 97 2421 1545 2229 Fragments arranged by size: 2421 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 2022 3423 1108 Size: 914 1401 3977 Fragments arranged by size: 3977 1401 914 PfoI T'CCnGG_A Cuts at: 2562 3009 2562 Size: 447 5845 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2679 2679 Size: 6292 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PspOMI G'GGCC_C Cuts at: 3243 3243 Size: 6292 PstI C_TGCA'G Cuts at: 830 3644 830 Size: 2814 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 3055 3055 Size: 6292 SacI G_AGCT'C Cuts at: 2410 2667 2410 Size: 257 6035 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SexAI A'CCwGG_T Cuts at: 2806 3408 2806 Size: 602 5690 SfcI C'TryA_G Cuts at: 826 1045 2298 3640 4775 5456 5647 826 Size: 219 1253 1342 1135 681 191 1471 Fragments arranged by size: 1471 1342 1253 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4396 5297 5559 820 Size: 197 926 2453 901 262 1553 Fragments arranged by size: 2453 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3310 4083 493 Size: 2817 773 2702 Fragments arranged by size: 2817 2702 773 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 2195 1517 Size: 678 5614 StyI C'CwwG_G Cuts at: 513 1065 3228 3731 513 Size: 552 2163 503 3074 Fragments arranged by size: 3074 2163 552 503 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 4319 4336 4489 4674 5119 325 Size: 1255 1131 1608 17 153 185 445 1498 Fragments arranged by size: 1608 1498 1255 1131 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4538 6250 96 Size: 274 80 33 51 1443 2561 1712 138 Fragments arranged by size: 2561 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 3378 3410 6252 6284 2780 Size: 32 566 32 2842 32 2788 Fragments arranged by size: 2842 2788 566 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3089 1316 Size: 1773 4519 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 2603 3032 3302 4421 2603 Size: 429 270 1119 4474 Fragments arranged by size: 4474 1119 429 270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI