(Linear) (Six-base) MAPSORT of: KIEE4505.seq Check: 2124 from: 1 to: 1587 KIEE4505.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 988 1587 Size: 988 599 Acc65I G'GTAC_C Cuts at: 0 1259 1330 1587 Size: 1259 71 257 Fragments arranged by size: 1259 257 71 AclI AA'CG_TT Cuts at: 0 1494 1587 Size: 1494 93 AfeI AGC'GCT Cuts at: 0 106 199 1587 Size: 106 93 1388 Fragments arranged by size: 1388 106 93 AflIII A'CryG_T Cuts at: 0 596 1587 Size: 596 991 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1258 1290 1587 Size: 1258 32 297 Fragments arranged by size: 1258 297 32 AlwNI CAG_nnn'CTG Cuts at: 0 1074 1418 1435 1587 Size: 1074 344 17 152 Fragments arranged by size: 1074 344 152 17 ApaI G_GGCC'C Cuts at: 0 1242 1587 Size: 1242 345 BanI G'GyrC_C Cuts at: 0 853 1259 1330 1587 Size: 853 406 71 257 Fragments arranged by size: 853 406 257 71 BanII G_rGCy'C Cuts at: 0 405 662 848 1242 1587 Size: 405 257 186 394 345 Fragments arranged by size: 405 394 345 257 186 BbsI GAAGACnn'nnnn_ Cuts at: 0 924 1587 Size: 924 663 BbvCI CC'TCA_GC Cuts at: 0 449 1522 1587 Size: 449 1073 65 Fragments arranged by size: 1073 449 65 BciVI GTATCCnnnnn_n' Cuts at: 0 516 760 817 1587 Size: 516 244 57 770 Fragments arranged by size: 770 516 244 57 Bme1580I G_kGCm'C Cuts at: 0 457 1242 1587 Size: 457 785 345 Fragments arranged by size: 785 457 345 BmrI ACTGGGnnnn_n' Cuts at: 0 869 1587 Size: 869 718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 436 1072 1587 Size: 289 147 636 515 Fragments arranged by size: 636 515 289 147 Bpu10I CC'TnA_GC Cuts at: 0 449 1337 1522 1587 Size: 449 888 185 65 Fragments arranged by size: 888 449 185 65 BsaAI yAC'GTr Cuts at: 0 1305 1587 Size: 1305 282 BsaBI GATnn'nnATC Cuts at: 0 944 1587 Size: 944 643 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 953 983 1373 1403 1587 Size: 953 30 390 30 184 Fragments arranged by size: 953 390 184 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 370 974 1587 Size: 370 604 613 Fragments arranged by size: 613 604 370 BsiEI CG_ry'CG Cuts at: 0 158 1587 Size: 158 1429 BsiHKAI G_wGCw'C Cuts at: 0 405 662 907 1587 Size: 405 257 245 680 Fragments arranged by size: 680 405 257 245 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1092 1587 Size: 1092 495 Bsp1286I G_dGCh'C Cuts at: 0 405 457 662 848 907 1242 1587 Size: 405 52 205 186 59 335 345 Fragments arranged by size: 405 345 335 205 186 59 52 BspHI T'CATG_A Cuts at: 0 662 899 1587 Size: 662 237 688 Fragments arranged by size: 688 662 237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 218 548 988 1587 Size: 218 330 440 599 Fragments arranged by size: 599 440 330 218 BsrFI r'CCGG_y Cuts at: 0 1228 1587 Size: 1228 359 BstEII G'GTnAC_C Cuts at: 0 1218 1587 Size: 1218 369 BstYI r'GATC_y Cuts at: 0 1001 1587 Size: 1001 586 EaeI y'GGCC_r Cuts at: 0 155 780 1587 Size: 155 625 807 Fragments arranged by size: 807 625 155 EagI C'GGCC_G Cuts at: 0 155 1587 Size: 155 1432 EarI CTCTTCn'nnn_ Cuts at: 0 1095 1587 Size: 1095 492 EcoICRI GAG'CTC Cuts at: 0 403 660 1587 Size: 403 257 927 Fragments arranged by size: 927 403 257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 436 1072 1587 Size: 289 147 636 515 Fragments arranged by size: 636 515 289 147 EcoNI CCTnn'n_nnAGG Cuts at: 0 184 1587 Size: 184 1403 EcoO109I rG'GnC_Cy Cuts at: 0 674 1239 1587 Size: 674 565 348 Fragments arranged by size: 674 565 348 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1044 1248 1587 Size: 1044 204 339 Fragments arranged by size: 1044 339 204 HaeII r_GCGC'y Cuts at: 0 108 201 1587 Size: 108 93 1386 Fragments arranged by size: 1386 108 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 136 168 791 823 1072 1104 1587 Size: 136 32 623 32 249 32 483 Fragments arranged by size: 623 483 249 136 32 32 32 HincII GTy'rAC Cuts at: 0 1466 1587 Size: 1466 121 KpnI G_GTAC'C Cuts at: 0 1263 1334 1587 Size: 1263 71 253 Fragments arranged by size: 1263 253 71 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 122 500 1016 1587 Size: 122 378 516 571 Fragments arranged by size: 571 516 378 122 MscI TGG'CCA Cuts at: 0 782 1587 Size: 782 805 MslI CAynn'nnrTG Cuts at: 0 306 1168 1587 Size: 306 862 419 Fragments arranged by size: 862 419 306 MspA1I CmG'CkG Cuts at: 0 1050 1511 1587 Size: 1050 461 76 Fragments arranged by size: 1050 461 76 NaeI GCC'GGC Cuts at: 0 1230 1587 Size: 1230 357 NdeI CA'TA_TG Cuts at: 0 636 1587 Size: 636 951 NgoMIV G'CCGG_C Cuts at: 0 1228 1587 Size: 1228 359 PflMI CCAn_nnn'nTGG Cuts at: 0 17 1418 1587 Size: 17 1401 169 Fragments arranged by size: 1401 169 17 PfoI T'CCnGG_A Cuts at: 0 557 1004 1587 Size: 557 447 583 Fragments arranged by size: 583 557 447 PpuMI rG'GwC_Cy Cuts at: 0 674 1587 Size: 674 913 PspOMI G'GGCC_C Cuts at: 0 1238 1587 Size: 1238 349 PvuII CAG'CTG Cuts at: 0 1050 1587 Size: 1050 537 SacI G_AGCT'C Cuts at: 0 405 662 1587 Size: 405 257 925 Fragments arranged by size: 925 405 257 SexAI A'CCwGG_T Cuts at: 0 801 1403 1587 Size: 801 602 184 Fragments arranged by size: 801 602 184 SfcI C'TryA_G Cuts at: 0 293 1587 Size: 293 1294 SnaBI TAC'GTA Cuts at: 0 1305 1587 Size: 1305 282 StuI AGG'CCT Cuts at: 0 190 1587 Size: 190 1397 StyI C'CwwG_G Cuts at: 0 1223 1587 Size: 1223 364 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 706 1587 Size: 706 881 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 775 807 1373 1405 1587 Size: 775 32 566 32 182 Fragments arranged by size: 775 566 182 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1084 1587 Size: 1084 503 XmnI GAAnn'nnTTC Cuts at: 0 598 1027 1297 1587 Size: 598 429 270 290 Fragments arranged by size: 598 429 290 270 Enzymes that do cut: AarI Acc65I AclI AfeI AflIII AloI AlwNI ApaI BanI BanII BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BstEII BstYI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV PflMI PfoI PpuMI PspOMI PvuII SacI SexAI SfcI SnaBI StuI StyI TaqII TstI Tth111I XmnI Enzymes that do not cut: AatII AccI AcuI AflII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaHI BsaWI BseYI BsgI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI SwaI TatI XbaI XcmI XhoI XmaI ZraI