(Linear) (Six-base) MAPSORT of: KIEE4577.seq Check: 2695 from: 1 to: 2292 KIEE4577.orf With 182 enzymes: SgfI * December 3, 2014 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2041 2173 2292 Size: 2041 132 119 Fragments arranged by size: 2041 132 119 Acc65I G'GTAC_C Cuts at: 0 1481 2292 Size: 1481 811 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 913 1264 2094 2292 Size: 913 351 830 198 Fragments arranged by size: 913 830 351 198 AfeI AGC'GCT Cuts at: 0 495 2101 2292 Size: 495 1606 191 Fragments arranged by size: 1606 495 191 AflII C'TTAA_G Cuts at: 0 379 2292 Size: 379 1913 AflIII A'CryG_T Cuts at: 0 1202 1373 1540 2292 Size: 1202 171 167 752 Fragments arranged by size: 1202 752 171 167 AleI CACnn'nnGTG Cuts at: 0 1444 2292 Size: 1444 848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1882 2292 Size: 1882 410 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 109 141 2292 Size: 109 32 2151 Fragments arranged by size: 2151 109 32 AlwNI CAG_nnn'CTG Cuts at: 0 450 1434 1458 2151 2292 Size: 450 984 24 693 141 Fragments arranged by size: 984 693 450 141 24 ApaI G_GGCC'C Cuts at: 0 834 1764 2292 Size: 834 930 528 Fragments arranged by size: 930 834 528 ApaLI G'TGCA_C Cuts at: 0 465 2292 Size: 465 1827 ApoI r'AATT_y Cuts at: 0 145 1804 2292 Size: 145 1659 488 Fragments arranged by size: 1659 488 145 AvaI C'yCGr_G Cuts at: 0 251 262 572 2200 2292 Size: 251 11 310 1628 92 Fragments arranged by size: 1628 310 251 92 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1758 1791 2292 Size: 1758 33 501 Fragments arranged by size: 1758 501 33 BamHI G'GATC_C Cuts at: 0 207 2292 Size: 207 2085 BanI G'GyrC_C Cuts at: 0 193 1367 1481 1682 2245 2292 Size: 193 1174 114 201 563 47 Fragments arranged by size: 1174 563 201 193 114 47 BanII G_rGCy'C Cuts at: 0 340 818 834 1093 1124 1764 1799 2292 Size: 340 478 16 259 31 640 35 493 Fragments arranged by size: 640 493 478 340 259 35 31 16 BbeI G_GCGC'C Cuts at: 0 1371 1686 2292 Size: 1371 315 606 Fragments arranged by size: 1371 606 315 BbsI GAAGACnn'nnnn_ Cuts at: 0 1623 2292 Size: 1623 669 BbvCI CC'TCA_GC Cuts at: 0 443 2292 Size: 443 1849 BciVI GTATCCnnnnn_n' Cuts at: 0 1786 2292 Size: 1786 506 Bme1580I G_kGCm'C Cuts at: 0 469 834 1764 2270 2292 Size: 469 365 930 506 22 Fragments arranged by size: 930 506 469 365 22 BmgBI CAC'GTC Cuts at: 0 353 2292 Size: 353 1939 BmrI ACTGGGnnnn_n' Cuts at: 0 456 1758 2139 2292 Size: 456 1302 381 153 Fragments arranged by size: 1302 456 381 153 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 187 250 1071 2292 Size: 187 63 821 1221 Fragments arranged by size: 1221 821 187 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 5 706 1084 1989 2026 2266 2292 Size: 5 701 378 905 37 240 26 Fragments arranged by size: 905 701 378 240 37 26 5 Bpu10I CC'TnA_GC Cuts at: 0 292 443 1666 2292 Size: 292 151 1223 626 Fragments arranged by size: 1223 626 292 151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 877 2292 Size: 877 1415 BsaI GGTCTCn'nnnn_ Cuts at: 0 252 1933 2292 Size: 252 1681 359 Fragments arranged by size: 1681 359 252 BsaAI yAC'GTr Cuts at: 0 1374 1543 1887 2292 Size: 1374 169 344 405 Fragments arranged by size: 1374 405 344 169 BsaHI Gr'CG_yC Cuts at: 0 1368 1683 2292 Size: 1368 315 609 Fragments arranged by size: 1368 609 315 BsaWI w'CCGG_w Cuts at: 0 140 2292 Size: 140 2152 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 109 139 2292 Size: 109 30 2153 Fragments arranged by size: 2153 109 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 534 808 1063 1327 2134 2269 2292 Size: 534 274 255 264 807 135 23 Fragments arranged by size: 807 534 274 264 255 135 23 BseYI C'CCAG_C Cuts at: 0 826 1512 2292 Size: 826 686 780 Fragments arranged by size: 826 780 686 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 871 1744 1831 2052 2292 Size: 484 387 873 87 221 240 Fragments arranged by size: 873 484 387 240 221 87 BsiEI CG_ry'CG Cuts at: 0 585 2292 Size: 585 1707 BsiHKAI G_wGCw'C Cuts at: 0 469 1799 2292 Size: 469 1330 493 Fragments arranged by size: 1330 493 469 BsmI GAATG_Cn' Cuts at: 0 401 2292 Size: 401 1891 Bsp1286I G_dGCh'C Cuts at: 0 340 469 818 834 1093 1124 1764 1799 Size: 340 129 349 16 259 31 640 35 Cuts at: 1799 2270 2292 Size: 471 22 Fragments arranged by size: 640 471 349 340 259 129 35 31 22 16 BspHI T'CATG_A Cuts at: 0 1574 1931 2292 Size: 1574 357 361 Fragments arranged by size: 1574 361 357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 881 1493 1535 1610 2041 2173 2292 Size: 881 612 42 75 431 132 119 Fragments arranged by size: 881 612 431 132 119 75 42 BsrBI CCG'CTC Cuts at: 0 101 2292 Size: 101 2191 BsrDI GCAATG_nn' Cuts at: 0 785 1070 1080 2292 Size: 785 285 10 1212 Fragments arranged by size: 1212 785 285 10 BssSI C'ACGA_G Cuts at: 0 1339 2292 Size: 1339 953 BstAPI GCAn_nnn'nTGC Cuts at: 0 939 2151 2292 Size: 939 1212 141 Fragments arranged by size: 1212 939 141 BstEII G'GTnAC_C Cuts at: 0 795 2292 Size: 795 1497 BstXI CCAn_nnnn'nTGG Cuts at: 0 61 1818 2292 Size: 61 1757 474 Fragments arranged by size: 1757 474 61 BstYI r'GATC_y Cuts at: 0 207 267 879 1152 1328 1385 2292 Size: 207 60 612 273 176 57 907 Fragments arranged by size: 907 612 273 207 176 60 57 BtgI C'CryG_G Cuts at: 0 113 1730 1854 2292 Size: 113 1617 124 438 Fragments arranged by size: 1617 438 124 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 489 1908 2292 Size: 489 1419 384 Fragments arranged by size: 1419 489 384 BtsI GCAGTG_nn' Cuts at: 0 409 535 667 2292 Size: 409 126 132 1625 Fragments arranged by size: 1625 409 132 126 DraIII CAC_nnn'GTG Cuts at: 0 989 2292 Size: 989 1303 EaeI y'GGCC_r Cuts at: 0 582 644 899 1469 2292 Size: 582 62 255 570 823 Fragments arranged by size: 823 582 570 255 62 EagI C'GGCC_G Cuts at: 0 582 2292 Size: 582 1710 EarI CTCTTCn'nnn_ Cuts at: 0 241 511 1423 1804 2292 Size: 241 270 912 381 488 Fragments arranged by size: 912 488 381 270 241 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 88 2292 Size: 88 2204 EcoICRI GAG'CTC Cuts at: 0 1797 2292 Size: 1797 495 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 5 706 913 1084 1264 1989 2026 2094 Size: 5 701 207 171 180 725 37 68 Cuts at: 2094 2266 2292 Size: 172 26 Fragments arranged by size: 725 701 207 180 172 171 68 37 26 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 361 1426 1669 2292 Size: 361 1065 243 623 Fragments arranged by size: 1065 623 361 243 EcoO109I rG'GnC_Cy Cuts at: 0 830 1431 1599 1702 1760 2169 2292 Size: 830 601 168 103 58 409 123 Fragments arranged by size: 830 601 409 168 123 103 58 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1191 2292 Size: 1191 1101 HaeII r_GCGC'y Cuts at: 0 497 1371 1686 2103 2292 Size: 497 874 315 417 189 Fragments arranged by size: 874 497 417 315 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 415 447 2158 2190 2233 2265 2292 Size: 415 32 1711 32 43 32 27 Fragments arranged by size: 1711 415 43 32 32 32 27 HincII GTy'rAC Cuts at: 0 168 1947 2292 Size: 168 1779 345 Fragments arranged by size: 1779 345 168 KasI G'GCGC_C Cuts at: 0 1367 1682 2292 Size: 1367 315 610 Fragments arranged by size: 1367 610 315 KpnI G_GTAC'C Cuts at: 0 1485 2292 Size: 1485 807 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 14 1260 2292 Size: 14 1246 1032 Fragments arranged by size: 1246 1032 14 MscI TGG'CCA Cuts at: 0 1471 2292 Size: 1471 821 MslI CAynn'nnrTG Cuts at: 0 1444 1816 2292 Size: 1444 372 476 Fragments arranged by size: 1444 476 372 MspA1I CmG'CkG Cuts at: 0 778 945 1043 1219 1350 1845 1856 1967 Size: 778 167 98 176 131 495 11 111 Cuts at: 1967 2046 2292 Size: 79 246 Fragments arranged by size: 778 495 246 176 167 131 111 98 79 11 NarI GG'CG_CC Cuts at: 0 1368 1683 2292 Size: 1368 315 609 Fragments arranged by size: 1368 609 315 NcoI C'CATG_G Cuts at: 0 113 1730 2292 Size: 113 1617 562 Fragments arranged by size: 1617 562 113 NotI GC'GGCC_GC Cuts at: 0 582 2292 Size: 582 1710 NspI r_CATG'y Cuts at: 0 571 751 807 1206 1920 2292 Size: 571 180 56 399 714 372 Fragments arranged by size: 714 571 399 372 180 56 PasI CC'CwG_GG Cuts at: 0 729 2292 Size: 729 1563 PciI A'CATG_T Cuts at: 0 1202 2292 Size: 1202 1090 PflMI CCAn_nnn'nTGG Cuts at: 0 662 1478 2292 Size: 662 816 814 Fragments arranged by size: 816 814 662 PfoI T'CCnGG_A Cuts at: 0 683 1778 1835 2003 2292 Size: 683 1095 57 168 289 Fragments arranged by size: 1095 683 289 168 57 PmlI CAC'GTG Cuts at: 0 1374 1543 2292 Size: 1374 169 749 Fragments arranged by size: 1374 749 169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 109 141 2292 Size: 109 32 2151 Fragments arranged by size: 2151 109 32 PpuMI rG'GwC_Cy Cuts at: 0 1599 2169 2292 Size: 1599 570 123 Fragments arranged by size: 1599 570 123 PspOMI G'GGCC_C Cuts at: 0 830 1760 2292 Size: 830 930 532 Fragments arranged by size: 930 830 532 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1516 1548 2292 Size: 1516 32 744 Fragments arranged by size: 1516 744 32 PstI C_TGCA'G Cuts at: 0 419 530 716 878 944 1142 1988 2292 Size: 419 111 186 162 66 198 846 304 Fragments arranged by size: 846 419 304 198 186 162 111 66 PvuII CAG'CTG Cuts at: 0 778 1043 1350 1845 1967 2292 Size: 778 265 307 495 122 325 Fragments arranged by size: 778 495 325 307 265 122 SacI G_AGCT'C Cuts at: 0 1799 2292 Size: 1799 493 SacII CC_GC'GG Cuts at: 0 1857 2292 Size: 1857 435 SapI GCTCTTCn'nnn_ Cuts at: 0 241 1804 2292 Size: 241 1563 488 Fragments arranged by size: 1563 488 241 SbfI CC_TGCA'GG Cuts at: 0 716 878 2292 Size: 716 162 1414 Fragments arranged by size: 1414 716 162 SfcI C'TryA_G Cuts at: 0 415 526 712 874 940 1138 1566 1649 Size: 415 111 186 162 66 198 428 83 Cuts at: 1649 1697 1984 2292 Size: 48 287 308 Fragments arranged by size: 428 415 308 287 198 186 162 111 83 66 48 SfoI GGC'GCC Cuts at: 0 1369 1684 2292 Size: 1369 315 608 Fragments arranged by size: 1369 608 315 SmaI CCC'GGG Cuts at: 0 253 574 2292 Size: 253 321 1718 Fragments arranged by size: 1718 321 253 SmlI C'TyrA_G Cuts at: 0 379 856 2200 2292 Size: 379 477 1344 92 Fragments arranged by size: 1344 477 379 92 SphI G_CATG'C Cuts at: 0 571 751 2292 Size: 571 180 1541 Fragments arranged by size: 1541 571 180 SrfI GCCC'GGGC Cuts at: 0 574 2292 Size: 574 1718 StyI C'CwwG_G Cuts at: 0 113 603 905 1730 1790 2228 2292 Size: 113 490 302 825 60 438 64 Fragments arranged by size: 825 490 438 302 113 64 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 239 2292 Size: 239 2053 TatI w'GTAC_w Cuts at: 0 1766 2292 Size: 1766 526 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1532 1564 2292 Size: 1532 32 728 Fragments arranged by size: 1532 728 32 Tth111I GACn'n_nGTC Cuts at: 0 388 2292 Size: 388 1904 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2001 2292 Size: 2001 291 XhoI C'TCGA_G Cuts at: 0 2200 2292 Size: 2200 92 XmaI C'CCGG_G Cuts at: 0 251 572 2292 Size: 251 321 1720 Fragments arranged by size: 1720 321 251 Enzymes that do cut: AarI Acc65I AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NotI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SacII SapI SbfI SfcI SfoI SmaI SmlI SphI SrfI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI AclI AgeI AhdI AscI AseI AsiSI AvrII BcgI BclI BfrBI BglI BglII BlpI BmtI BsaBI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NruI NsiI PacI PmeI PshAI PsiI PspXI PvuI RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI StuI SwaI XbaI XmnI ZraI