Restriction map for
pFN21AE3923
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
BalI BamHI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspHI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstF5I BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoRV
EcoT22I FokI FseI FspI HgaI
HindIII HpaI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MscI MslI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 172 TCCGGA T^CCGGA
AlwI 849 GATCC GGATC(4/5)
Aor51HI 338 AGCGCT AGC^GCT
Asp700I 745 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 831 GAGCAC G[AT]GC[AT]^C
AvaII 1294 GGTCC G^G[AT]CC
AvrII 307 CCTAGG C^CTAGG
BanII 303 GAGCCC G[GA]GC[CT]^C
BclI 1047 TGATCA T^GATCA
BcnI 80 CCGGG CC^[GC]GG
BlnI 307 CCTAGG C^CTAGG
BpmI 493 CTGGAG CTGGAG(16/14)
BsaI 409 GAGACC GGTCTC(1/5)
BsaWI 172 TCCGGA [AT]^CCGG[AT]
BseAI 172 TCCGGA T^CCGGA
BsiHKAI 831 GAGCAC G[AT]GC[AT]^C
Bsp1407I 881 TGTACA T^GTACA
BspEI 172 TCCGGA T^CCGGA
BsrFI 5 GCCGGC [GA]^CCGG[CT]
BsrGI 881 TGTACA T^GTACA
BstBI 752 TTCGAA TT^CGAA
BstEII 290 GGTCACC G^GT[ACGT]ACC
BstPI 290 GGTCACC G^GT[ACGT]ACC
BstYI 848 AGATCC [GA]^GATC[CT]
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
DraII 1293 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 31 CGGCCA [CT]^GGCC[GA]
Eco47III 338 AGCGCT AGC^GCT
EcoNI 489 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1293 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 290 GGTCACC G^GT[ACGT]ACC
EcoRI 1081 GAATTC G^AATTC
EcoT14I 307 CCTAGG C^C[AT][AT]GG
FauI 38 CCCGC CCCGC(4/6)
FbaI 1047 TGATCA T^GATCA
HincII 1070 GTCGAC GT[CT]^[GA]AC
HindII 1070 GTCGAC GT[CT]^[GA]AC
HinfI 761 GACTC G^A[ACGT]TC
MflI 848 AGATCC [GA]^GATC[CT]
MroI 172 TCCGGA T^CCGGA
NaeI 5 GCCGGC GCC^GGC
NciI 80 CCGGG CC^[GC]GG
NgoMIV 5 GCCGGC G^CCGGC
NspV 752 TTCGAA TT^CGAA
PleI 761 GACTC GAGTC(4/5)
PpuMI 1293 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 18 CAGCTG CAG^CTG
SalI 1070 GTCGAC G^TCGAC
SfuI 752 TTCGAA TT^CGAA
SpeI 210 ACTAGT A^CTAGT
SspBI 881 TGTACA T^GTACA
StyI 307 CCTAGG C^C[AT][AT]GG
XhoII 848 AGATCC [GA]^GATC[CT]
XmnI 745 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 39 CCGC CCGC(-3/-1)
77 CCGC
AcyI 8 GGCGCC G[GA]^CG[CT]C
83 GGCGCC
AflIII 363 ACATGT A^C[GA][CT]GT
768 ACATGT
AluI 19 AGCT AG^CT
1268 AGCT
BanI 8 GGCGCC G^G[CT][GA]CC
83 GGCGCC
BbeI 8 GGCGCC GGCGC^C
83 GGCGCC
BbsI 206 GAAGAC GAAGAC(2/6)
427 GAAGAC
BmyI 303 GAGCCC G[AGT]GC[ACT]^C
831 GAGCAC
BpuAI 206 GAAGAC GAAGAC(2/6)
427 GAAGAC
BsaHI 8 GGCGCC G[GA]^CG[CT]C
83 GGCGCC
BsaJI 95 CCTCGG C^C[ACGT][ACGT]GG
307 CCTAGG
BseRI 1270 CTCCTC GAGGAG(10/8)
496 GAGGAG
BsiYI 489 CCTCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1297 CCCCTGCCTGG
BslI 489 CCTCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1297 CCCCTGCCTGG
BsmAI 409 GAGAC GTCTC(1/5)
1226 GTCTC
BsmFI 759 GGGAC GGGAC(10/14)
1295 GTCCC
BsmI 621 GAATGC GAATGC(1/-1)
871 GCATTC
Bsp1286I 303 GAGCCC G[AGT]GC[ACT]^C
831 GAGCAC
BspLU11I 363 ACATGT A^CATGT
768 ACATGT
BspMI 1149 ACCTGC ACCTGC(4/8)
1183 ACCTGC
BstNI 492 CCTGG CC^[AT]GG
1303 CCTGG
Cfr13I 717 GGGCC G^G[ACGT]CC
1294 GGTCC
EcoRII 492 CCTGG ^CC[AT]GG
1303 CCTGG
Hin1I 8 GGCGCC G[GA]^CG[CT]C
83 GGCGCC
KasI 8 GGCGCC G^GCGCC
83 GGCGCC
MspA1I 18 CAGCTG C[AC]G^C[GT]G
39 CCGCTG
MvaI 492 CCTGG CC^[AT]GG
1303 CCTGG
NarI 8 GGCGCC GG^CGCC
83 GGCGCC
NspI 363 ACATGT [GA]CATG^[CT]
768 ACATGT
Sau96I 717 GGGCC G^G[ACGT]CC
1294 GGTCC
SfaNI 654 GATGC GCATC(5/9)
1018 GATGC
SfoI 8 GGCGCC GGC^GCC
83 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccI AcsI AfaI ApoI BbvI
BfaI BsrI BssKI Cac8I CfoI
DdeI DpnII Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
ScrFI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I