################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AccI AccIII AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 206 GACGTC GACGT^C AclI 735 AACGTT AA^CGTT AseI 288 ATTAAT AT^TAAT AsnI 288 ATTAAT AT^TAAT Asp700I 723 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 249 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 577 CCCGAG C^[CT]CG[GA]G BamHI 953 GGATCC G^GATCC BbeI 100 GGCGCC GGCGC^C BbsI 1018 GTCTTC GAAGAC(2/6) BcnI 669 CCCGG CC^[GC]GG BfaI 1357 CTAG C^TAG BglII 485 AGATCT A^GATCT BlpI 651 GCTGAGC GC^T[ACGT]AGC Bpu1102I 651 GCTGAGC GC^T[ACGT]AGC BpuAI 1018 GTCTTC GAAGAC(2/6) BsgI 796 GTGCAG GTGCAG(16/14) BsiEI 1252 CGGCCG CG[GA][CT]^CG BsoBI 577 CCCGAG C^[CT]CG[GA]G BsrFI 1254 GCCGGC [GA]^CCGG[CT] BssSI 82 CTCGTG CACGAG(-5/-1) BstEII 864 GGTCACC G^GT[ACGT]ACC BstPI 864 GGTCACC G^GT[ACGT]ACC BstXI 894 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 651 GCTGAGC GC^T[ACGT]AGC CfoI 101 GCGC GCG^C Cfr10I 1254 GCCGGC [GA]^CCGG[CT] DrdI 1495 GACTCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1252 CGGCCG C^GGCCG EclXI 1252 CGGCCG C^GGCCG Eco52I 1252 CGGCCG C^GGCCG EcoO65I 864 GGTCACC G^GT[ACGT]ACC FauI 97 GCGGG CCCGC(4/6) HaeII 100 GGCGCC [GA]GCGC^[CT] HhaI 101 GCGC GCG^C HinP1I 101 GCGC G^CGC HincII 1282 GTCAAC GT[CT]^[GA]AC HindII 1282 GTCAAC GT[CT]^[GA]AC KasI 100 GGCGCC G^GCGCC MaeI 1357 CTAG C^TAG MseI 289 TTAA T^TAA MslI 543 CATTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1254 GCCGGC GCC^GGC NarI 100 GGCGCC GG^CGCC NciI 669 CCCGG CC^[GC]GG NcoI 881 CCATGG C^CATGG NgoMIV 1254 GCCGGC G^CCGGC PflFI 249 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 288 ATTAAT AT^TAAT Psp1406I 735 AACGTT AA^CGTT SacI 839 GAGCTC GAGCT^C SbfI 1122 CCTGCAGG CCTGCA^GG SfoI 100 GGCGCC GGC^GCC SmlI 984 CTTGAG C^T[CT][GA]AG Sse8387I 1122 CCTGCAGG CCTGCA^GG TfiI 43 GATTC G^A[AT]TC Tru9I 289 TTAA T^TAA Tth111I 249 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 723 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 96 CGCG CG^CG 793 CGCG AlwNI 562 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 646 CAGCTGCTG BanI 100 GGCGCC G^G[CT][GA]CC 1276 GGCACC BpmI 388 CTCCAG CTGGAG(16/14) 826 CTGGAG BsaI 1053 GAGACC GGTCTC(1/5) 1414 GAGACC BsmI 147 GAATGC GAATGC(1/-1) 1166 GCATTC BsrDI 368 CATTGC GCAATG(2/0) 1269 CATTGC BstAPI 327 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 355 GCAATTTCTGC BstF5I 807 CATCC GGATG(2/0) 1209 CATCC BstUI 96 CGCG CG^CG 793 CGCG DsaI 504 CCGTGG C^C[GA][CT]GG 881 CCATGG EcoT14I 494 CCAAGG C^C[AT][AT]GG 881 CCATGG FokI 807 CATCC GGATG(9/13) 1209 CATCC HapII 670 CCGG C^CGG 1255 CCGG HgaI 422 GACGC GACGC(5/10) 449 GCGTC HpaII 670 CCGG C^CGG 1255 CCGG MspI 670 CCGG C^CGG 1255 CCGG MvnI 96 CGCG CG^CG 793 CGCG PstI 1123 CTGCAG CTGCA^G 1191 CTGCAG PvuII 646 CAGCTG CAG^CTG 1499 CAGCTG SfcI 1123 CTGCAG C^T[GA][CT]AG 1191 CTGCAG StuI 170 AGGCCT AGG^CCT 1528 AGGCCT StyI 494 CCAAGG C^C[AT][AT]GG 881 CCATGG TaqI 1001 TCGA T^CGA 1493 TCGA TthHB8I 1001 TCGA T^CGA 1493 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI HaeIII Hin1I HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI XhoII