(Circular) (Six-base) MAPSORT of: pFN21AE4490.seq Check: 9173 from: 1 to: 6238 pFN21AE4490 6238 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2515 2854 2515 Size: 339 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2215 4048 278 Size: 53 83 186 936 679 1833 2468 Fragments arranged by size: 2468 1833 936 679 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 2741 4365 4738 1563 Size: 1178 1624 373 3063 Fragments arranged by size: 3063 1624 1178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4301 5685 5953 1632 Size: 2669 1384 268 1917 Fragments arranged by size: 2669 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2547 1184 Size: 1363 4875 AlwNI CAG_nnn'CTG Cuts at: 2572 2656 3981 5553 2572 Size: 84 1325 1572 3257 Fragments arranged by size: 3257 1572 1325 84 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 3547 3773 3925 6034 1493 Size: 2054 226 152 2109 1697 Fragments arranged by size: 2109 2054 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2294 4792 160 Size: 872 1262 2498 1606 Fragments arranged by size: 2498 1606 1262 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2582 3563 1870 Size: 73 639 981 4545 Fragments arranged by size: 4545 981 639 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3145 3178 3145 Size: 33 6205 BamHI G'GATC_C Cuts at: 2958 3568 3935 2958 Size: 610 367 5261 Fragments arranged by size: 5261 610 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 3281 3559 5014 618 Size: 325 217 627 318 1176 278 1455 1842 Fragments arranged by size: 1842 1455 1176 627 325 318 278 217 BanII G_rGCy'C Cuts at: 1391 2848 3087 3318 3557 1391 Size: 1457 239 231 239 4072 Fragments arranged by size: 4072 1457 239 239 231 BbeI G_GCGC'C Cuts at: 2109 2109 Size: 6238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3016 928 Size: 971 1117 4150 Fragments arranged by size: 4150 1117 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2490 6233 2490 Size: 3743 2495 BlpI GC'TnA_GC Cuts at: 2657 3655 2657 Size: 998 5240 Bme1580I G_kGCm'C Cuts at: 1790 4299 5455 1790 Size: 2509 1156 2573 Fragments arranged by size: 2573 2509 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2571 3969 4927 Size: 416 53 83 399 24 1154 1398 958 Cuts at: 4927 442 Size: 1753 Fragments arranged by size: 1753 1398 1154 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2852 4898 1239 Size: 1139 474 2046 2579 Fragments arranged by size: 2579 2046 1139 474 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3010 4363 5228 5526 3010 Size: 1353 865 298 3722 Fragments arranged by size: 3722 1353 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3052 3413 4901 882 Size: 617 1553 361 1488 2219 Fragments arranged by size: 2219 1553 1488 617 361 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2212 2454 4045 Size: 53 83 186 936 573 106 242 1591 Cuts at: 4045 4427 5062 275 Size: 382 635 1451 Fragments arranged by size: 1591 1451 936 635 573 382 242 186 106 83 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2616 2646 3281 3311 1178 Size: 30 165 30 1213 30 635 30 4105 Fragments arranged by size: 4105 1213 635 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2043 2075 2107 2792 2821 3056 3308 1186 Size: 857 32 32 685 29 235 252 4116 Fragments arranged by size: 4116 857 685 252 235 32 32 29 BseYI C'CCAG_C Cuts at: 2590 2954 5441 5937 2590 Size: 364 2487 496 2891 Fragments arranged by size: 2891 2487 496 364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2822 1163 Size: 573 1086 4579 Fragments arranged by size: 4579 1086 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3260 3688 4449 5477 1540 Size: 275 186 1259 428 761 1028 2301 Fragments arranged by size: 2301 1259 1028 761 428 275 186 BsiHKAI G_wGCw'C Cuts at: 2472 2848 3048 4299 4384 5455 2472 Size: 376 200 1251 85 1071 3255 Fragments arranged by size: 3255 1251 1071 376 200 85 BsmI GAATG_Cn' Cuts at: 2158 3171 3749 3842 2158 Size: 1013 578 93 4554 Fragments arranged by size: 4554 1013 578 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2472 2848 3048 3087 3318 3557 4299 Size: 399 682 376 200 39 231 239 742 Cuts at: 4299 4384 5455 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 682 399 376 239 231 200 85 39 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2515 2700 2854 3593 844 Size: 1671 185 154 739 3489 Fragments arranged by size: 3489 1671 739 185 154 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2372 3273 4733 4907 65 Size: 1183 1124 901 1460 174 1396 Fragments arranged by size: 1460 1396 1183 1124 901 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3259 4882 5069 1700 Size: 110 1449 1623 187 2869 Fragments arranged by size: 2869 1623 1449 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 2087 4298 5310 2087 Size: 2211 1012 3015 Fragments arranged by size: 3015 2211 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2338 2366 794 Size: 1544 28 4666 Fragments arranged by size: 4666 1544 28 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 2869 1184 Size: 1685 4553 BstXI CCAn_nnnn'nTGG Cuts at: 2906 2906 Size: 6238 BstYI r'GATC_y Cuts at: 1972 2490 2505 2958 2973 3568 3935 4320 4337 Size: 518 15 453 15 595 367 385 17 Cuts at: 4337 5789 5875 5887 6233 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 595 518 453 385 367 346 86 17 15 15 12 BtgI C'CryG_G Cuts at: 513 1065 2509 2886 513 Size: 552 1444 377 3865 Fragments arranged by size: 3865 1444 552 377 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3169 487 Size: 683 56 1943 3556 Fragments arranged by size: 3556 1943 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3754 4548 4568 3205 Size: 549 794 20 4875 Fragments arranged by size: 4875 794 549 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3506 5245 809 Size: 507 2190 1739 1802 Fragments arranged by size: 2190 1802 1739 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 2609 3177 Size: 54 1425 50 275 18 513 266 568 Cuts at: 3177 3257 3685 4574 6203 8 Size: 80 428 889 1629 43 Fragments arranged by size: 1629 1425 889 568 513 428 275 266 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3257 3685 1537 Size: 275 1445 428 4090 Fragments arranged by size: 4090 1445 428 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3101 3326 3483 4168 1353 Size: 1748 225 157 685 3423 Fragments arranged by size: 3423 1748 685 225 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2804 4825 5199 5345 387 Size: 1064 138 1215 2021 374 146 1280 Fragments arranged by size: 2021 1280 1215 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2846 2846 Size: 6238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2852 4301 4898 5685 5953 1239 Size: 393 746 474 1449 597 787 268 1524 Fragments arranged by size: 1524 1449 787 746 597 474 393 268 EcoO109I rG'GnC_Cy Cuts at: 2782 3105 3148 2782 Size: 323 43 5872 Fragments arranged by size: 5872 323 43 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2109 5385 1678 Size: 431 3276 2531 Fragments arranged by size: 3276 2531 431 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2418 2450 3051 3083 4879 Size: 32 135 32 846 32 601 32 1796 Cuts at: 4879 4911 4953 4985 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1796 846 601 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3289 3582 3828 669 Size: 988 281 1351 293 246 3079 Fragments arranged by size: 3079 1351 988 293 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KasI G'GCGC_C Cuts at: 2105 2105 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3324 5352 5536 1305 Size: 2019 2028 184 2007 Fragments arranged by size: 2028 2019 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2345 2611 3179 10 Size: 54 1425 343 513 266 568 3069 Fragments arranged by size: 3069 1425 568 513 343 266 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 4196 4555 4714 518 Size: 648 1386 1644 359 159 2042 Fragments arranged by size: 2042 1644 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2653 2859 3256 3506 3654 4331 5479 5724 Size: 1047 206 397 250 148 677 1148 245 Cuts at: 5724 1606 Size: 2120 Fragments arranged by size: 2120 1148 1047 677 397 250 245 206 148 NaeI GCC'GGC Cuts at: 3261 3261 Size: 6238 NarI GG'CG_CC Cuts at: 2106 2106 Size: 6238 NcoI C'CATG_G Cuts at: 513 1065 2886 513 Size: 552 1821 3865 Fragments arranged by size: 3865 1821 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NgoMIV G'CCGG_C Cuts at: 3259 3259 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3596 5141 1132 Size: 97 2367 1545 2229 Fragments arranged by size: 2367 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3144 1348 Size: 1796 4442 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6238 PfoI T'CCnGG_A Cuts at: 2673 2673 Size: 6238 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2782 3105 3148 2782 Size: 323 43 5872 Fragments arranged by size: 5872 323 43 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3461 3493 3461 Size: 32 6206 PstI C_TGCA'G Cuts at: 830 3132 3200 3590 830 Size: 2302 68 390 3478 Fragments arranged by size: 3478 2302 390 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 2653 3506 2653 Size: 853 5385 SacI G_AGCT'C Cuts at: 2848 2848 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SanDI GG'GwC_CC Cuts at: 3105 3148 3105 Size: 43 6195 SbfI CC_TGCA'GG Cuts at: 3132 3590 3132 Size: 458 5780 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 3128 3196 3586 4721 5402 5593 826 Size: 219 2083 68 390 1135 681 191 1471 Fragments arranged by size: 2083 1471 1135 681 390 219 191 68 SfoI GGC'GCC Cuts at: 2107 2107 Size: 6238 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2989 4342 5243 5505 820 Size: 197 926 1046 1353 901 262 1553 Fragments arranged by size: 1553 1353 1046 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 2177 3535 1517 Size: 660 1358 4220 Fragments arranged by size: 4220 1358 660 StyI C'CwwG_G Cuts at: 513 1065 2499 2886 3677 513 Size: 552 1434 387 791 3074 Fragments arranged by size: 3074 1434 791 552 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2040 2512 4265 4282 4435 4620 5065 Size: 1255 460 472 1753 17 153 185 445 Cuts at: 5065 325 Size: 1498 Fragments arranged by size: 1753 1498 1255 472 460 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2993 4484 6196 Size: 274 80 33 51 1443 1016 1491 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 1712 1491 1443 1016 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 6198 6230 2616 Size: 32 3550 32 2624 Fragments arranged by size: 3550 2624 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2257 1316 Size: 941 5297 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 2732 4367 2732 Size: 1635 4603 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2213 4046 276 Size: 53 83 186 936 679 1833 2468 Fragments arranged by size: 2468 1833 936 679 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI