(Linear) (Six-base) MAPSORT of: KIEE4490.seq Check: 7882 from: 1 to: 1533 KIEE4490.orf With 182 enzymes: SgfI * March 22, 2012 18:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 510 849 1533 Size: 510 339 684 Fragments arranged by size: 684 510 339 AatII G_ACGT'C Cuts at: 0 210 1533 Size: 210 1323 AclI AA'CG_TT Cuts at: 0 736 1533 Size: 736 797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 542 1533 Size: 542 991 AlwNI CAG_nnn'CTG Cuts at: 0 567 651 1533 Size: 567 84 882 Fragments arranged by size: 882 567 84 AseI AT'TA_AT Cuts at: 0 289 1533 Size: 289 1244 AvaI C'yCGr_G Cuts at: 0 577 1533 Size: 577 956 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1140 1173 1533 Size: 1140 33 360 Fragments arranged by size: 1140 360 33 BamHI G'GATC_C Cuts at: 0 953 1533 Size: 953 580 BanI G'GyrC_C Cuts at: 0 100 1276 1533 Size: 100 1176 257 Fragments arranged by size: 1176 257 100 BanII G_rGCy'C Cuts at: 0 843 1082 1313 1533 Size: 843 239 231 220 Fragments arranged by size: 843 239 231 220 BbeI G_GCGC'C Cuts at: 0 104 1533 Size: 104 1429 BbsI GAAGACnn'nnnn_ Cuts at: 0 1011 1533 Size: 1011 522 BglII A'GATC_T Cuts at: 0 485 1533 Size: 485 1048 BlpI GC'TnA_GC Cuts at: 0 652 1533 Size: 652 881 BmrI ACTGGGnnnn_n' Cuts at: 0 566 1533 Size: 566 967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 847 1533 Size: 373 474 686 Fragments arranged by size: 686 474 373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1005 1533 Size: 1005 528 BsaI GGTCTCn'nnnn_ Cuts at: 0 1047 1408 1533 Size: 1047 361 125 Fragments arranged by size: 1047 361 125 BsaHI Gr'CG_yC Cuts at: 0 101 207 449 1533 Size: 101 106 242 1084 Fragments arranged by size: 1084 242 106 101 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 611 641 1276 1306 1533 Size: 611 30 635 30 227 Fragments arranged by size: 635 611 227 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 38 70 102 787 816 1051 1303 1533 Size: 38 32 32 685 29 235 252 230 Fragments arranged by size: 685 252 235 230 38 32 32 29 BseYI C'CCAG_C Cuts at: 0 585 949 1533 Size: 585 364 584 Fragments arranged by size: 585 584 364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 817 1533 Size: 817 716 BsiEI CG_ry'CG Cuts at: 0 1255 1533 Size: 1255 278 BsiHKAI G_wGCw'C Cuts at: 0 467 843 1043 1533 Size: 467 376 200 490 Fragments arranged by size: 490 467 376 200 BsmI GAATG_Cn' Cuts at: 0 153 1166 1533 Size: 153 1013 367 Fragments arranged by size: 1013 367 153 Bsp1286I G_dGCh'C Cuts at: 0 467 843 1043 1082 1313 1533 Size: 467 376 200 39 231 220 Fragments arranged by size: 467 376 231 220 200 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 510 695 849 1533 Size: 510 185 154 684 Fragments arranged by size: 684 510 185 154 BsrDI GCAATG_nn' Cuts at: 0 367 1268 1533 Size: 367 901 265 Fragments arranged by size: 901 367 265 BsrFI r'CCGG_y Cuts at: 0 1254 1533 Size: 1254 279 BssSI C'ACGA_G Cuts at: 0 82 1533 Size: 82 1451 BstAPI GCAn_nnn'nTGC Cuts at: 0 333 361 1533 Size: 333 28 1172 Fragments arranged by size: 1172 333 28 BstEII G'GTnAC_C Cuts at: 0 864 1533 Size: 864 669 BstXI CCAn_nnnn'nTGG Cuts at: 0 901 1533 Size: 901 632 BstYI r'GATC_y Cuts at: 0 485 500 953 968 1533 Size: 485 15 453 15 565 Fragments arranged by size: 565 485 453 15 15 BtgI C'CryG_G Cuts at: 0 504 881 1533 Size: 504 377 652 Fragments arranged by size: 652 504 377 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1164 1533 Size: 1164 369 BtsI GCAGTG_nn' Cuts at: 0 1200 1533 Size: 1200 333 DrdI GACnn_nn'nnGTC Cuts at: 0 1501 1533 Size: 1501 32 EaeI y'GGCC_r Cuts at: 0 338 604 1172 1252 1533 Size: 338 266 568 80 281 Fragments arranged by size: 568 338 281 266 80 EagI C'GGCC_G Cuts at: 0 1252 1533 Size: 1252 281 EarI CTCTTCn'nnn_ Cuts at: 0 1096 1321 1478 1533 Size: 1096 225 157 55 Fragments arranged by size: 1096 225 157 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 799 1533 Size: 799 734 EcoICRI GAG'CTC Cuts at: 0 841 1533 Size: 841 692 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 847 1533 Size: 373 474 686 Fragments arranged by size: 686 474 373 EcoO109I rG'GnC_Cy Cuts at: 0 777 1100 1143 1533 Size: 777 323 43 390 Fragments arranged by size: 777 390 323 43 HaeII r_GCGC'y Cuts at: 0 104 1533 Size: 104 1429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 413 445 1046 1078 1533 Size: 413 32 601 32 455 Fragments arranged by size: 601 455 413 32 32 HincII GTy'rAC Cuts at: 0 1284 1533 Size: 1284 249 KasI G'GCGC_C Cuts at: 0 100 1533 Size: 100 1433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1319 1533 Size: 1319 214 MscI TGG'CCA Cuts at: 0 340 606 1174 1533 Size: 340 266 568 359 Fragments arranged by size: 568 359 340 266 MslI CAynn'nnrTG Cuts at: 0 547 1533 Size: 547 986 MspA1I CmG'CkG Cuts at: 0 648 854 1251 1501 1533 Size: 648 206 397 250 32 Fragments arranged by size: 648 397 250 206 32 NaeI GCC'GGC Cuts at: 0 1256 1533 Size: 1256 277 NarI GG'CG_CC Cuts at: 0 101 1533 Size: 101 1432 NcoI C'CATG_G Cuts at: 0 881 1533 Size: 881 652 NgoMIV G'CCGG_C Cuts at: 0 1254 1533 Size: 1254 279 PasI CC'CwG_GG Cuts at: 0 1139 1533 Size: 1139 394 PfoI T'CCnGG_A Cuts at: 0 668 1533 Size: 668 865 PpuMI rG'GwC_Cy Cuts at: 0 777 1100 1143 1533 Size: 777 323 43 390 Fragments arranged by size: 777 390 323 43 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1456 1488 1533 Size: 1456 32 45 Fragments arranged by size: 1456 45 32 PstI C_TGCA'G Cuts at: 0 1127 1195 1533 Size: 1127 68 338 Fragments arranged by size: 1127 338 68 PvuII CAG'CTG Cuts at: 0 648 1501 1533 Size: 648 853 32 Fragments arranged by size: 853 648 32 SacI G_AGCT'C Cuts at: 0 843 1533 Size: 843 690 SanDI GG'GwC_CC Cuts at: 0 1100 1143 1533 Size: 1100 43 390 Fragments arranged by size: 1100 390 43 SbfI CC_TGCA'GG Cuts at: 0 1127 1533 Size: 1127 406 SfcI C'TryA_G Cuts at: 0 1123 1191 1533 Size: 1123 68 342 Fragments arranged by size: 1123 342 68 SfoI GGC'GCC Cuts at: 0 102 1533 Size: 102 1431 SmlI C'TyrA_G Cuts at: 0 984 1533 Size: 984 549 StuI AGG'CCT Cuts at: 0 172 1530 1533 Size: 172 1358 3 Fragments arranged by size: 1358 172 3 StyI C'CwwG_G Cuts at: 0 494 881 1533 Size: 494 387 652 Fragments arranged by size: 652 494 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 35 507 1533 Size: 35 472 1026 Fragments arranged by size: 1026 472 35 TatI w'GTAC_w Cuts at: 0 988 1533 Size: 988 545 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 611 643 1533 Size: 611 32 890 Fragments arranged by size: 890 611 32 Tth111I GACn'n_nGTC Cuts at: 0 252 1533 Size: 252 1281 XmnI GAAnn'nnTTC Cuts at: 0 727 1533 Size: 727 806 ZraI GAC'GTC Cuts at: 0 208 1533 Size: 208 1325 Enzymes that do cut: AarI AatII AclI AlfI AlwNI AseI AvaI BaeI BamHI BanI BanII BbeI BbsI BglII BlpI BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PfoI PpuMI PsrI PstI PvuII SacI SanDI SbfI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XmnI ZraI Enzymes that do not cut: AccI Acc65I AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI