(Linear) (Six-base) MAPSORT of: KIEE3931.seq Check: 9884 from: 1 to: 1635 KIEE3931.orf With 182 enzymes: SgfI * March 22, 2012 16:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1367 1635 Size: 1367 268 AccI GT'mk_AC Cuts at: 0 464 1635 Size: 464 1171 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 670 740 1635 Size: 466 204 70 895 Fragments arranged by size: 895 466 204 70 AfeI AGC'GCT Cuts at: 0 1147 1635 Size: 1147 488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1145 1635 Size: 1145 490 AlwNI CAG_nnn'CTG Cuts at: 0 1374 1449 1635 Size: 1374 75 186 Fragments arranged by size: 1374 186 75 ApaI G_GGCC'C Cuts at: 0 1179 1288 1635 Size: 1179 109 347 Fragments arranged by size: 1179 347 109 ApaLI G'TGCA_C Cuts at: 0 1546 1635 Size: 1546 89 BanI G'GyrC_C Cuts at: 0 525 1402 1508 1612 1635 Size: 525 877 106 104 23 Fragments arranged by size: 877 525 106 104 23 BanII G_rGCy'C Cuts at: 0 1179 1288 1435 1459 1635 Size: 1179 109 147 24 176 Fragments arranged by size: 1179 176 147 109 24 BbeI G_GCGC'C Cuts at: 0 1512 1635 Size: 1512 123 BbsI GAAGACnn'nnnn_ Cuts at: 0 17 143 1635 Size: 17 126 1492 Fragments arranged by size: 1492 126 17 BfrBI ATG'CAT Cuts at: 0 759 1635 Size: 759 876 BlpI GC'TnA_GC Cuts at: 0 1453 1635 Size: 1453 182 Bme1580I G_kGCm'C Cuts at: 0 575 1179 1288 1405 1550 1615 1635 Size: 575 604 109 117 145 65 20 Fragments arranged by size: 604 575 145 117 109 65 20 BmrI ACTGGGnnnn_n' Cuts at: 0 1180 1215 1454 1635 Size: 1180 35 239 181 Fragments arranged by size: 1180 239 181 35 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 332 1635 Size: 332 1303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 251 619 865 1363 1635 Size: 235 16 368 246 498 272 Fragments arranged by size: 498 368 272 246 235 16 BsaI GGTCTCn'nnnn_ Cuts at: 0 874 1635 Size: 874 761 BsaAI yAC'GTr Cuts at: 0 576 1545 1635 Size: 576 969 90 Fragments arranged by size: 969 576 90 BsaHI Gr'CG_yC Cuts at: 0 744 1509 1635 Size: 744 765 126 Fragments arranged by size: 765 744 126 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 49 58 133 136 145 181 184 Size: 46 3 9 75 3 9 36 3 Cuts at: 184 208 217 232 550 643 1314 1635 Size: 24 9 15 318 93 671 321 Fragments arranged by size: 671 321 318 93 75 46 36 24 15 9 9 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 92 767 771 818 1235 1364 1504 1635 Size: 92 675 4 47 417 129 140 131 Fragments arranged by size: 675 417 140 131 129 92 47 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 628 1635 Size: 565 63 1007 Fragments arranged by size: 1007 565 63 BsiEI CG_ry'CG Cuts at: 0 1157 1197 1635 Size: 1157 40 438 Fragments arranged by size: 1157 438 40 BsiHKAI G_wGCw'C Cuts at: 0 1435 1550 1604 1635 Size: 1435 115 54 31 Fragments arranged by size: 1435 115 54 31 BsmBI CGTCTCn'nnnn_ Cuts at: 0 751 1635 Size: 751 884 Bsp1286I G_dGCh'C Cuts at: 0 575 1179 1288 1405 1435 1459 1550 1604 Size: 575 604 109 117 30 24 91 54 Cuts at: 1604 1615 1635 Size: 11 20 Fragments arranged by size: 604 575 117 109 91 54 30 24 20 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 74 347 1367 1635 Size: 74 273 1020 268 Fragments arranged by size: 1020 273 268 74 BsrBI CCG'CTC Cuts at: 0 852 1116 1190 1635 Size: 852 264 74 445 Fragments arranged by size: 852 445 264 74 BsrFI r'CCGG_y Cuts at: 0 1196 1349 1635 Size: 1196 153 286 Fragments arranged by size: 1196 286 153 BstAPI GCAn_nnn'nTGC Cuts at: 0 1374 1635 Size: 1374 261 BstXI CCAn_nnnn'nTGG Cuts at: 0 483 941 1635 Size: 483 458 694 Fragments arranged by size: 694 483 458 BstYI r'GATC_y Cuts at: 0 234 1635 Size: 234 1401 Bsu36I CC'TnA_GG Cuts at: 0 178 1635 Size: 178 1457 BtgI C'CryG_G Cuts at: 0 788 1301 1635 Size: 788 513 334 Fragments arranged by size: 788 513 334 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1324 1635 Size: 1324 311 BtsI GCAGTG_nn' Cuts at: 0 253 611 1635 Size: 253 358 1024 Fragments arranged by size: 1024 358 253 DraIII CAC_nnn'GTG Cuts at: 0 522 1635 Size: 522 1113 EaeI y'GGCC_r Cuts at: 0 347 1154 1194 1635 Size: 347 807 40 441 Fragments arranged by size: 807 441 347 40 EagI C'GGCC_G Cuts at: 0 1154 1194 1635 Size: 1154 40 441 Fragments arranged by size: 1154 441 40 EarI CTCTTCn'nnn_ Cuts at: 0 47 224 457 464 646 716 908 1635 Size: 47 177 233 7 182 70 192 727 Fragments arranged by size: 727 233 192 182 177 70 47 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 319 336 1093 1635 Size: 319 17 757 542 Fragments arranged by size: 757 542 319 17 EcoICRI GAG'CTC Cuts at: 0 1433 1635 Size: 1433 202 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 251 466 619 670 740 865 1363 Size: 235 16 215 153 51 70 125 498 Cuts at: 1363 1635 Size: 272 Fragments arranged by size: 498 272 235 215 153 125 70 51 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 392 1635 Size: 392 1243 EcoO109I rG'GnC_Cy Cuts at: 0 174 1175 1285 1635 Size: 174 1001 110 350 Fragments arranged by size: 1001 350 174 110 HaeII r_GCGC'y Cuts at: 0 1149 1479 1512 1635 Size: 1149 330 33 123 Fragments arranged by size: 1149 330 123 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 928 960 1005 1037 1306 1338 1635 Size: 928 32 45 32 269 32 297 Fragments arranged by size: 928 297 269 45 32 32 32 KasI G'GCGC_C Cuts at: 0 1508 1635 Size: 1508 127 MscI TGG'CCA Cuts at: 0 349 1635 Size: 349 1286 MslI CAynn'nnrTG Cuts at: 0 481 971 1635 Size: 481 490 664 Fragments arranged by size: 664 490 481 MspA1I CmG'CkG Cuts at: 0 92 345 679 771 790 924 1310 1383 Size: 92 253 334 92 19 134 386 73 Cuts at: 1383 1635 Size: 252 Fragments arranged by size: 386 334 253 252 134 92 92 73 19 NaeI GCC'GGC Cuts at: 0 1198 1351 1635 Size: 1198 153 284 Fragments arranged by size: 1198 284 153 NarI GG'CG_CC Cuts at: 0 1509 1635 Size: 1509 126 NgoMIV G'CCGG_C Cuts at: 0 1196 1349 1635 Size: 1196 153 286 Fragments arranged by size: 1196 286 153 NsiI A_TGCA'T Cuts at: 0 761 1635 Size: 761 874 NspI r_CATG'y Cuts at: 0 293 1635 Size: 293 1342 PasI CC'CwG_GG Cuts at: 0 1179 1288 1635 Size: 1179 109 347 Fragments arranged by size: 1179 347 109 PflMI CCAn_nnn'nTGG Cuts at: 0 388 1635 Size: 388 1247 PfoI T'CCnGG_A Cuts at: 0 499 881 1635 Size: 499 382 754 Fragments arranged by size: 754 499 382 PmlI CAC'GTG Cuts at: 0 576 1545 1635 Size: 576 969 90 Fragments arranged by size: 969 576 90 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 731 763 1635 Size: 46 32 653 32 872 Fragments arranged by size: 872 653 46 32 32 PpuMI rG'GwC_Cy Cuts at: 0 174 1635 Size: 174 1461 PspOMI G'GGCC_C Cuts at: 0 1175 1284 1635 Size: 1175 109 351 Fragments arranged by size: 1175 351 109 PstI C_TGCA'G Cuts at: 0 344 569 929 1635 Size: 344 225 360 706 Fragments arranged by size: 706 360 344 225 PvuII CAG'CTG Cuts at: 0 92 345 771 924 1635 Size: 92 253 426 153 711 Fragments arranged by size: 711 426 253 153 92 SacI G_AGCT'C Cuts at: 0 1435 1635 Size: 1435 200 SacII CC_GC'GG Cuts at: 0 791 1635 Size: 791 844 SapI GCTCTTCn'nnn_ Cuts at: 0 457 646 716 1635 Size: 457 189 70 919 Fragments arranged by size: 919 457 189 70 SfcI C'TryA_G Cuts at: 0 340 565 925 1635 Size: 340 225 360 710 Fragments arranged by size: 710 360 340 225 SfoI GGC'GCC Cuts at: 0 1510 1635 Size: 1510 125 StyI C'CwwG_G Cuts at: 0 623 1471 1607 1635 Size: 623 848 136 28 Fragments arranged by size: 848 623 136 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 395 424 1234 1635 Size: 395 29 810 401 Fragments arranged by size: 810 401 395 29 TatI w'GTAC_w Cuts at: 0 551 1635 Size: 551 1084 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 1378 1410 1635 Size: 97 32 1249 32 225 Fragments arranged by size: 1249 225 97 32 32 Tth111I GACn'n_nGTC Cuts at: 0 244 1635 Size: 244 1391 XbaI T'CTAG_A Cuts at: 0 237 1635 Size: 237 1398 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 342 1635 Size: 342 1293 XmnI GAAnn'nnTTC Cuts at: 0 451 1635 Size: 451 1184 Enzymes that do cut: AarI AccI AcuI AfeI AlfI AlwNI ApaI ApaLI BanI BanII BbeI BbsI BfrBI BlpI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BglI BglII BmgBI BmtI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XhoI XmaI ZraI