Restriction map for
pFN21AE4493
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbvCI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 75 GTGCAC G^TGCAC
AocI 1305 CCTGAGG CC^T[ACGT]AGG
ApaLI 75 GTGCAC G^TGCAC
AvaI 1264 CTCGAG C^[CT]CG[GA]G
BamHI 482 GGATCC G^GATCC
BbsI 739 GAAGAC GAAGAC(2/6)
BglII 818 AGATCT A^GATCT
BpuAI 739 GAAGAC GAAGAC(2/6)
BsgI 273 CTGCAC GTGCAG(16/14)
BsmAI 1483 GTCTC GTCTC(1/5)
BsmI 887 GCATTC GAATGC(1/-1)
BsoBI 1264 CTCGAG C^[CT]CG[GA]G
BspMI 1130 ACCTGC ACCTGC(4/8)
BsrBI 438 CCGCTC CCGCTC(-3/-3)
BsrDI 980 GCAATG GCAATG(2/0)
BsrFI 43 GCCGGC [GA]^CCGG[CT]
BssHII 264 GCGCGC G^CGCGC
BstAPI 980 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1305 CCTGAGG CC^T[ACGT]AGG
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
DraII 912 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1120 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 583 GACACGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 1305 CCTGAGG CC^T[ACGT]AGG
EcoO109I 912 AGGCCCC [GA]G^G[ACGT]CC[CT]
HphI 580 GGTGA GGTGA(8/7)
MaeII 704 ACGT A^CGT
MslI 1377 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 43 GCCGGC GCC^GGC
NcoI 920 CCATGG C^CATGG
NgoMIV 43 GCCGGC G^CCGGC
NlaIII 921 CATG CATG^
PaeR7I 1264 CTCGAG C^TCGAG
PflMI 488 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1084 GAGCTC GAGCT^C
SapI 1249 GAAGAGC GCTCTTC(1/4)
StuI 491 AGGCCT AGG^CCT
Van91I 488 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1264 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwI 483 GATCC GGATC(4/5)
482 GGATC
AlwNI 710 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1334 CAGGCTCTG
AvaII 458 GGACC G^G[AT]CC
1128 GGACC
BanII 780 GGGCTC G[GA]GC[CT]^C
1084 GAGCTC
BbeI 566 GGCGCC GGCGC^C
929 GGCGCC
BcgI 1044 CGACGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
824 GCAAGAGTTTCG
BglI 86 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
120 GCCCCCTGGGC
BsiEI 41 CGGCCG CG[GA][CT]^CG
318 CGACCG
BssSI 423 CACGAG CACGAG(-5/-1)
1068 CACGAG
BstXI 462 CCAGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
758 CCAACAAGGTGG
BstYI 482 GGATCC [GA]^GATC[CT]
818 AGATCT
DdeI 678 CTCAG C^T[ACGT]AG
1306 CTGAG
DpnII 483 GATC ^GATC
819 GATC
EaeI 41 CGGCCG [CT]^GGCC[GA]
698 CGGCCA
EarI 880 GAAGAG CTCTTC(1/4)
1249 GAAGAG
EcoT14I 488 CCAAGG C^C[AT][AT]GG
920 CCATGG
HgaI 331 GACGC GACGC(5/10)
1036 GACGC
KasI 566 GGCGCC G^GCGCC
929 GGCGCC
Ksp632I 880 GAAGAG CTCTTC(1/4)
1249 GAAGAG
KspI 134 CCGCGG CCGC^GG
321 CCGCGG
MboI 483 GATC ^GATC
819 GATC
MflI 482 GGATCC [GA]^GATC[CT]
818 AGATCT
NarI 566 GGCGCC GG^CGCC
929 GGCGCC
NdeII 483 GATC ^GATC
819 GATC
PstI 310 CTGCAG CTGCA^G
975 CTGCAG
PvuII 451 CAGCTG CAG^CTG
661 CAGCTG
SacII 134 CCGCGG CCGC^GG
321 CCGCGG
Sau3AI 483 GATC ^GATC
819 GATC
SexAI 617 ACCAGGT A^CC[AT]GGT
1508 ACCTGGT
SfcI 310 CTGCAG C^T[GA][CT]AG
975 CTGCAG
SfoI 566 GGCGCC GGC^GCC
929 GGCGCC
SmlI 637 CTCAAG C^T[CT][GA]AG
1264 CTCGAG
StyI 488 CCAAGG C^C[AT][AT]GG
920 CCATGG
TaqI 995 TCGA T^CGA
1265 TCGA
TthHB8I 995 TCGA T^CGA
1265 TCGA
XhoII 482 GGATCC [GA]^GATC[CT]
818 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DsaI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PleI RsaI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI