Restriction map for
pFN21AE4493
(Circular) (Six-base) MAPSORT of: pFN21AE4493.seq Check: 437 from: 1 to: 6244
pFN21AE4493 6244 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 3175 4307 5691 5959 1632
Size: 935 608 1132 1384 268 1917
Fragments arranged by size:
1917 1384 1132 935 608 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 5143 6120 1203
Size: 3940 977 1327
Fragments arranged by size:
3940 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 4973 4973
Size: 6244
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 2720 3344 3987 5559 2720
Size: 624 643 1572 3405
Fragments arranged by size:
3405 1572 643 624
ApaLI G'TGCA_C
Cuts at: 2080 4301 5457 2080
Size: 2221 1156 2867
Fragments arranged by size:
2867 2221 1156
ApoI r'AATT_y
Cuts at: 1493 3553 3779 3931 6040 1493
Size: 2060 226 152 2109 1697
Fragments arranged by size:
2109 2060 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 3569 1870
Size: 73 1326 300 4545
Fragments arranged by size:
4545 1326 300 73
BamHI G'GATC_C
Cuts at: 2487 3574 3941 2487
Size: 1087 367 4790
Fragments arranged by size:
4790 1087 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2176 2571 2651 2934
Size: 325 217 627 312 77 395 80 283
Cuts at: 2934 2954 3565 5020 618
Size: 20 611 1455 1842
Fragments arranged by size:
1842 1455 627 611 395 325 312 283
217 80 77 20
BanII G_rGCy'C
Cuts at: 1391 2789 3093 3563 1391
Size: 1398 304 470 4072
Fragments arranged by size:
4072 1398 470 304
BbeI G_GCGC'C
Cuts at: 2575 2938 2575
Size: 363 5881
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2751 928
Size: 971 852 4421
Fragments arranged by size:
4421 971 852
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2818 2852 3038 3072 4435 4469 1903
Size: 34 881 34 186 34 1363 34 3678
Fragments arranged by size:
3678 1363 881 186 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2097 2131 4855
Size: 107 122 71 631 605 425 34 2724
Cuts at: 4855 136
Size: 1525
Fragments arranged by size:
2724 1525 631 605 425 122 107 71
34
BglII A'GATC_T
Cuts at: 2823 6239 2823
Size: 3416 2828
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2102 3126 4305 5461 1790
Size: 294 18 1024 1179 1156 2573
Fragments arranged by size:
2573 1179 1156 1024 294 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2965 3105 3975
Size: 416 53 83 399 24 1548 140 870
Cuts at: 3975 4933 442
Size: 958 1753
Fragments arranged by size:
1753 1548 958 870 416 399 140 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2252 3032 3077 4904 1239
Size: 839 174 780 45 1827 2579
Fragments arranged by size:
2579 1827 839 780 174 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 4369 5234 5532 2627
Size: 1742 865 298 3339
Fragments arranged by size:
3339 1742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4907 882
Size: 617 3408 2219
Fragments arranged by size:
3408 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2337 2572 2935 3042
Size: 53 83 186 936 804 235 363 107
Cuts at: 3042 4051 4433 5068 275
Size: 1009 382 635 1451
Fragments arranged by size:
1451 1009 936 804 635 382 363 235
186 107 83 53
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6019
Fragments arranged by size:
6019 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6244
BseYI C'CCAG_C
Cuts at: 2256 3332 5447 5943 2256
Size: 1076 2115 496 2557
Fragments arranged by size:
2557 2115 1076 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 1163
Size: 573 527 5144
Fragments arranged by size:
5144 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2326 3694 4455 5483 1540
Size: 275 186 48 277 1368 761 1028 2301
Fragments arranged by size:
2301 1368 1028 761 277 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2084 2951 3093 3513 4305 4390 5461 2084
Size: 867 142 420 792 85 1071 2867
Fragments arranged by size:
2867 1071 867 792 420 142 85
BsmI GAATG_Cn'
Cuts at: 2892 3755 3848 2892
Size: 863 93 5288
Fragments arranged by size:
5288 863 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2102 2789 2951 3093 3126 3513
Size: 399 294 18 687 162 142 33 387
Cuts at: 3513 3563 4305 4390 5461 1391
Size: 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 399 387 294 162
142 85 50 33 18
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 4133 5863 4133
Size: 1730 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3144 3599 844
Size: 2300 455 3489
Fragments arranged by size:
3489 2300 455
BsrBI CCG'CTC
Cuts at: 1394 1823 2445 4131 1394
Size: 429 622 1686 3507
Fragments arranged by size:
3507 1686 622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2992 4739 4913 65
Size: 1183 1744 1747 174 1396
Fragments arranged by size:
1747 1744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 4888 5075 1700
Size: 110 238 2840 187 2869
Fragments arranged by size:
2869 2840 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6244
BssHII G'CGCG_C
Cuts at: 1925 2269 1925
Size: 344 5900
BssSI C'ACGA_G
Cuts at: 2428 3073 4304 5316 2428
Size: 645 1231 1012 3356
Fragments arranged by size:
3356 1231 1012 645
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2991 794
Size: 2197 4047
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstXI CCAn_nnnn'nTGG
Cuts at: 2474 2770 2474
Size: 296 5948
BstYI r'GATC_y
Cuts at: 1972 2487 2823 3574 3941 4326 4343 5795 5881
Size: 515 336 751 367 385 17 1452 86
Cuts at: 5881 5893 6239 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 751 515 385 367 346 336
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3311 3311
Size: 6244
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2326 2814 2925 3442 513
Size: 552 939 135 187 488 111 517 3315
Fragments arranged by size:
3315 939 552 517 488 187 135 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2326 487
Size: 683 56 1100 4405
Fragments arranged by size:
4405 1100 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3199 3269 3369 3760 4554 4574 3124
Size: 75 70 100 391 794 20 4794
Fragments arranged by size:
4794 794 391 100 75 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2607 2642 529
Size: 35 2043 35 4131
Fragments arranged by size:
4131 2043 35 35
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DraIII CAC_nnn'GTG
Cuts at: 3130 3130
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2594 5251 809
Size: 507 1278 2657 1802
Fragments arranged by size:
2657 1802 1278 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2703 3691
Size: 54 1425 50 275 18 216 657 988
Cuts at: 3691 4580 6209 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 988 889 657 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3691 1537
Size: 275 234 1645 4090
Fragments arranged by size:
4090 1645 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2880 3249 4174 1353
Size: 1527 369 925 3423
Fragments arranged by size:
3423 1527 925 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4831 5205 5351 387
Size: 1064 138 3242 374 146 1280
Fragments arranged by size:
3242 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3091 3091
Size: 6244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2252 2567 3032 3077 3175 4307
Size: 393 446 174 315 465 45 98 1132
Cuts at: 4307 4904 5691 5959 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1132 787 597 465 446 393 315
268 174 98 45
EcoO109I rG'GnC_Cy
Cuts at: 2918 2918
Size: 6244
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 6244
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 2306 2575 2938 5391 1678
Size: 628 269 363 2453 2531
Fragments arranged by size:
2531 2453 628 363 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4885 4917 4959 4991 1373
Size: 32 135 32 3313 32 42 32 2626
Fragments arranged by size:
3313 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KasI G'GCGC_C
Cuts at: 2571 2934 2571
Size: 363 5881
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 3072 5358 5542 1305
Size: 1482 285 2286 184 2007
Fragments arranged by size:
2286 2007 1482 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4422
Fragments arranged by size:
4422 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 4202 4561 4720 518
Size: 648 2220 816 359 159 2042
Fragments arranged by size:
2220 2042 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 2328 2458 2668 2979 3660 4337 5485
Size: 535 187 130 210 311 681 677 1148
Cuts at: 5485 5730 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 681 677 535 311 245 210
187 130
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 6244
NarI GG'CG_CC
Cuts at: 2572 2935 2572
Size: 363 5881
NcoI C'CATG_G
Cuts at: 513 1065 2004 2925 513
Size: 552 939 921 3832
Fragments arranged by size:
3832 939 921 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 3602 5147 1132
Size: 97 2373 1545 2229
Fragments arranged by size:
2373 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2129 2403 2467 1348
Size: 781 274 64 5125
Fragments arranged by size:
5125 781 274 64
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2499 1108
Size: 1391 4853
PfoI T'CCnGG_A
Cuts at: 2229 3009 2229
Size: 780 5464
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspXI vC'TCGA_Gb
Cuts at: 3269 3269
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2319 2984 3596 830
Size: 1489 665 612 3478
Fragments arranged by size:
3478 1489 665 612
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2458 2668 2458
Size: 210 6034
SacI G_AGCT'C
Cuts at: 3093 3093
Size: 6244
SacII CC_GC'GG
Cuts at: 2142 2329 2142
Size: 187 6057
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SapI GCTCTTCn'nnn_
Cuts at: 3249 3249
Size: 6244
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SexAI A'CCwGG_T
Cuts at: 2622 3513 2622
Size: 891 5353
SfcI C'TryA_G
Cuts at: 826 1045 2315 2980 3592 4727 5408 5599 826
Size: 219 1270 665 612 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 665 612 219 191
SfoI GGC'GCC
Cuts at: 2573 2936 2573
Size: 363 5881
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 3269 4348 5249 5511 820
Size: 197 926 699 627 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 699 627 262 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 2498 1517
Size: 981 5263
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 2925 3683 513
Size: 552 939 489 432 758 3074
Fragments arranged by size:
3074 939 758 552 489 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2197 2300 2706 2984 3474 3481
Size: 1255 537 80 103 406 278 490 7
Cuts at: 3481 4271 4288 4441 4626 5071 325
Size: 790 17 153 185 445 1498
Fragments arranged by size:
1498 1255 790 537 490 445 406 278
185 153 103 80 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4490 6202 96
Size: 274 80 33 51 1443 2513 1712 138
Fragments arranged by size:
2513 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 6204 6236 2873
Size: 32 3299 32 2881
Fragments arranged by size:
3299 2881 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 3269 1943
Size: 1326 4918
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SanDI SfiI SrfI SwaI XcmI