(Linear) (Six-base) MAPSORT of: KIEE4493.seq Check: 6927 from: 1 to: 1539 KIEE4493.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 1170 1539 Size: 562 608 369 Fragments arranged by size: 608 562 369 AlwNI CAG_nnn'CTG Cuts at: 0 715 1339 1539 Size: 715 624 200 Fragments arranged by size: 715 624 200 ApaLI G'TGCA_C Cuts at: 0 75 1539 Size: 75 1464 AvaI C'yCGr_G Cuts at: 0 1264 1539 Size: 1264 275 BamHI G'GATC_C Cuts at: 0 482 1539 Size: 482 1057 BanI G'GyrC_C Cuts at: 0 94 171 566 646 929 949 1539 Size: 94 77 395 80 283 20 590 Fragments arranged by size: 590 395 283 94 80 77 20 BanII G_rGCy'C Cuts at: 0 784 1088 1539 Size: 784 304 451 Fragments arranged by size: 784 451 304 BbeI G_GCGC'C Cuts at: 0 570 933 1539 Size: 570 363 606 Fragments arranged by size: 606 570 363 BbsI GAAGACnn'nnnn_ Cuts at: 0 746 1539 Size: 746 793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 813 847 1033 1067 1539 Size: 813 34 186 34 472 Fragments arranged by size: 813 472 186 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 92 126 1539 Size: 92 34 1413 Fragments arranged by size: 1413 92 34 BglII A'GATC_T Cuts at: 0 818 1539 Size: 818 721 Bme1580I G_kGCm'C Cuts at: 0 79 97 1121 1539 Size: 79 18 1024 418 Fragments arranged by size: 1024 418 79 18 BmrI ACTGGGnnnn_n' Cuts at: 0 960 1100 1539 Size: 960 140 439 Fragments arranged by size: 960 439 140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 247 1027 1072 1539 Size: 73 174 780 45 467 Fragments arranged by size: 780 467 174 73 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 1539 Size: 622 917 BsaHI Gr'CG_yC Cuts at: 0 332 567 930 1037 1539 Size: 332 235 363 107 502 Fragments arranged by size: 502 363 332 235 107 BseYI C'CCAG_C Cuts at: 0 251 1327 1539 Size: 251 1076 212 Fragments arranged by size: 1076 251 212 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 1539 Size: 258 1281 BsiEI CG_ry'CG Cuts at: 0 44 321 1539 Size: 44 277 1218 Fragments arranged by size: 1218 277 44 BsiHKAI G_wGCw'C Cuts at: 0 79 946 1088 1508 1539 Size: 79 867 142 420 31 Fragments arranged by size: 867 420 142 79 31 BsmI GAATG_Cn' Cuts at: 0 887 1539 Size: 887 652 Bsp1286I G_dGCh'C Cuts at: 0 79 97 784 946 1088 1121 1508 1539 Size: 79 18 687 162 142 33 387 31 Fragments arranged by size: 687 387 162 142 79 33 31 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1139 1539 Size: 1139 400 BsrBI CCG'CTC Cuts at: 0 440 1539 Size: 440 1099 BsrDI GCAATG_nn' Cuts at: 0 987 1539 Size: 987 552 BsrFI r'CCGG_y Cuts at: 0 43 1539 Size: 43 1496 BssHII G'CGCG_C Cuts at: 0 264 1539 Size: 264 1275 BssSI C'ACGA_G Cuts at: 0 423 1068 1539 Size: 423 645 471 Fragments arranged by size: 645 471 423 BstAPI GCAn_nnn'nTGC Cuts at: 0 986 1539 Size: 986 553 BstXI CCAn_nnnn'nTGG Cuts at: 0 469 765 1539 Size: 469 296 774 Fragments arranged by size: 774 469 296 BstYI r'GATC_y Cuts at: 0 482 818 1539 Size: 482 336 721 Fragments arranged by size: 721 482 336 Bsu36I CC'TnA_GG Cuts at: 0 1306 1539 Size: 1306 233 BtgI C'CryG_G Cuts at: 0 134 321 809 920 1437 1539 Size: 134 187 488 111 517 102 Fragments arranged by size: 517 488 187 134 111 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 321 1539 Size: 321 1218 BtsI GCAGTG_nn' Cuts at: 0 1119 1194 1264 1364 1539 Size: 1119 75 70 100 175 Fragments arranged by size: 1119 175 100 75 70 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 602 637 1539 Size: 602 35 902 Fragments arranged by size: 902 602 35 DraIII CAC_nnn'GTG Cuts at: 0 1125 1539 Size: 1125 414 DrdI GACnn_nn'nnGTC Cuts at: 0 589 1539 Size: 589 950 EaeI y'GGCC_r Cuts at: 0 41 698 1539 Size: 41 657 841 Fragments arranged by size: 841 657 41 EagI C'GGCC_G Cuts at: 0 41 1539 Size: 41 1498 EarI CTCTTCn'nnn_ Cuts at: 0 875 1244 1539 Size: 875 369 295 Fragments arranged by size: 875 369 295 EcoICRI GAG'CTC Cuts at: 0 1086 1539 Size: 1086 453 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 247 562 1027 1072 1170 1539 Size: 73 174 315 465 45 98 369 Fragments arranged by size: 465 369 315 174 98 73 45 EcoO109I rG'GnC_Cy Cuts at: 0 913 1539 Size: 913 626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1257 1539 Size: 1257 282 HaeII r_GCGC'y Cuts at: 0 301 570 933 1539 Size: 301 269 363 606 Fragments arranged by size: 606 363 301 269 KasI G'GCGC_C Cuts at: 0 566 929 1539 Size: 566 363 610 Fragments arranged by size: 610 566 363 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 782 1067 1539 Size: 782 285 472 Fragments arranged by size: 782 472 285 MslI CAynn'nnrTG Cuts at: 0 1381 1539 Size: 1381 158 MspA1I CmG'CkG Cuts at: 0 136 323 453 663 974 1539 Size: 136 187 130 210 311 565 Fragments arranged by size: 565 311 210 187 136 130 NaeI GCC'GGC Cuts at: 0 45 1539 Size: 45 1494 NarI GG'CG_CC Cuts at: 0 567 930 1539 Size: 567 363 609 Fragments arranged by size: 609 567 363 NcoI C'CATG_G Cuts at: 0 920 1539 Size: 920 619 NgoMIV G'CCGG_C Cuts at: 0 43 1539 Size: 43 1496 PasI CC'CwG_GG Cuts at: 0 124 398 462 1539 Size: 124 274 64 1077 Fragments arranged by size: 1077 274 124 64 PflMI CCAn_nnn'nTGG Cuts at: 0 494 1539 Size: 494 1045 PfoI T'CCnGG_A Cuts at: 0 224 1004 1539 Size: 224 780 535 Fragments arranged by size: 780 535 224 PspXI vC'TCGA_Gb Cuts at: 0 1264 1539 Size: 1264 275 PstI C_TGCA'G Cuts at: 0 314 979 1539 Size: 314 665 560 Fragments arranged by size: 665 560 314 PvuII CAG'CTG Cuts at: 0 453 663 1539 Size: 453 210 876 Fragments arranged by size: 876 453 210 SacI G_AGCT'C Cuts at: 0 1088 1539 Size: 1088 451 SacII CC_GC'GG Cuts at: 0 137 324 1539 Size: 137 187 1215 Fragments arranged by size: 1215 187 137 SapI GCTCTTCn'nnn_ Cuts at: 0 1244 1539 Size: 1244 295 SexAI A'CCwGG_T Cuts at: 0 617 1508 1539 Size: 617 891 31 Fragments arranged by size: 891 617 31 SfcI C'TryA_G Cuts at: 0 310 975 1539 Size: 310 665 564 Fragments arranged by size: 665 564 310 SfoI GGC'GCC Cuts at: 0 568 931 1539 Size: 568 363 608 Fragments arranged by size: 608 568 363 SmlI C'TyrA_G Cuts at: 0 637 1264 1539 Size: 637 627 275 Fragments arranged by size: 637 627 275 StuI AGG'CCT Cuts at: 0 493 1539 Size: 493 1046 StyI C'CwwG_G Cuts at: 0 488 920 1539 Size: 488 432 619 Fragments arranged by size: 619 488 432 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 112 192 295 701 979 1469 1476 1539 Size: 112 80 103 406 278 490 7 63 Fragments arranged by size: 490 406 278 112 103 80 63 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 868 900 1539 Size: 868 32 639 Fragments arranged by size: 868 639 32 XhoI C'TCGA_G Cuts at: 0 1264 1539 Size: 1264 275 Enzymes that do cut: AcuI AlwNI ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BglII Bme1580I BmrI BpmI BpuEI BsaHI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PspXI PstI PvuII SacI SacII SapI SexAI SfcI SfoI SmlI StuI StyI TaqII TstI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmBI BspEI BspHI BsrGI BstBI BstEII BstZ17I ClaI DraI EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI