Restriction map for
pFN21AE1308
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AccII 63 CGCG CG^CG
AcsI 585 GAATTT [GA]^AATT[CT]
ApaI 9 GGGCCC GGGCC^C
ApoI 585 GAATTT [GA]^AATT[CT]
BciVI 1405 GGATAC GTATCC(6/5)
BglII 507 AGATCT A^GATCT
Bsp1407I 1550 TGTACA T^GTACA
BspHI 737 TCATGA T^CATGA
BsrGI 1550 TGTACA T^GTACA
BstUI 63 CGCG CG^CG
BstYI 507 AGATCT [GA]^GATC[CT]
HgaI 64 GCGTC GACGC(5/10)
MflI 507 AGATCT [GA]^GATC[CT]
MspA1I 151 CAGCTG C[AC]G^C[GT]G
MvnI 63 CGCG CG^CG
NdeI 1163 CATATG CA^TATG
PvuII 151 CAGCTG CAG^CTG
RcaI 737 TCATGA T^CATGA
SmlI 836 CTCAAG C^T[CT][GA]AG
SspBI 1550 TGTACA T^GTACA
XbaI 255 TCTAGA T^CTAGA
XhoII 507 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 311 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1494 GAAGAAGTTC
BbsI 1225 GAAGAC GAAGAC(2/6)
1486 GAAGAC
BcnI 12 CCCGG CC^[GC]GG
107 CCGGG
BfaI 256 CTAG C^TAG
1451 CTAG
BpuAI 1225 GAAGAC GAAGAC(2/6)
1486 GAAGAC
BsaI 694 GAGACC GGTCTC(1/5)
822 GAGACC
BsgI 478 CTGCAC GTGCAG(16/14)
1193 GTGCAG
BsrFI 1325 GCCGGT [GA]^CCGG[CT]
1457 ACCGGC
CfoI 85 GCGC GCG^C
95 GCGC
Cfr10I 1325 GCCGGT [GA]^CCGG[CT]
1457 ACCGGC
DraI 1068 TTTAAA TTT^AAA
1480 TTTAAA
DraII 1004 GGGACCT [GA]G^G[ACGT]CC[CT]
1311 AGGTCCC
EcoO109I 1004 GGGACCT [GA]G^G[ACGT]CC[CT]
1311 AGGTCCC
EcoT14I 1000 CCAAGG C^C[AT][AT]GG
1025 CCAAGG
HaeII 84 GGCGCT [GA]GCGC^[CT]
94 GGCGCT
HhaI 85 GCGC GCG^C
95 GCGC
HinP1I 85 GCGC G^CGC
95 GCGC
HincII 234 GTCAAC GT[CT]^[GA]AC
903 GTCAAC
HindII 234 GTCAAC GT[CT]^[GA]AC
903 GTCAAC
MaeI 256 CTAG C^TAG
1451 CTAG
MslI 481 CACTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1275 CATTGTTGTG
NciI 12 CCCGG CC^[GC]GG
107 CCGGG
NspI 878 GCATGT [GA]CATG^[CT]
1319 GCATGT
PpuMI 1004 GGGACCT [GA]G^G[AT]CC[CT]
1311 AGGTCCC
StyI 1000 CCAAGG C^C[AT][AT]GG
1025 CCAAGG
Tsp509I 586 AATT ^AATT
1089 AATT
XmnI 311 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1494 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I