(Linear) (Six-base) MAPSORT of: KIEE1308.seq Check: 1234 from: 1 to: 1572 KIEE1308.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 83 376 700 1417 1513 1572 Size: 83 293 324 717 96 59 Fragments arranged by size: 717 324 293 96 83 59 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 548 1572 Size: 548 1024 AlwNI CAG_nnn'CTG Cuts at: 0 162 333 1051 1572 Size: 162 171 718 521 Fragments arranged by size: 718 521 171 162 ApaI G_GGCC'C Cuts at: 0 13 1572 Size: 13 1559 ApoI r'AATT_y Cuts at: 0 585 1572 Size: 585 987 BanII G_rGCy'C Cuts at: 0 13 20 226 1448 1572 Size: 13 7 206 1222 124 Fragments arranged by size: 1222 206 124 13 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 1232 1493 1572 Size: 1232 261 79 Fragments arranged by size: 1232 261 79 BciVI GTATCCnnnnn_n' Cuts at: 0 1399 1572 Size: 1399 173 BglII A'GATC_T Cuts at: 0 507 1572 Size: 507 1065 Bme1580I G_kGCm'C Cuts at: 0 13 1572 Size: 13 1559 BmrI ACTGGGnnnn_n' Cuts at: 0 16 603 1572 Size: 16 587 969 Fragments arranged by size: 969 587 16 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 717 1572 Size: 717 855 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 478 783 1067 1572 Size: 208 270 305 284 505 Fragments arranged by size: 505 305 284 270 208 Bpu10I CC'TnA_GC Cuts at: 0 1382 1572 Size: 1382 190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 821 1572 Size: 821 751 BsaI GGTCTCn'nnnn_ Cuts at: 0 688 816 1572 Size: 688 128 756 Fragments arranged by size: 756 688 128 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 216 375 703 706 719 856 882 1572 Size: 216 159 328 3 13 137 26 690 Fragments arranged by size: 690 328 216 159 137 26 13 3 BseYI C'CCAG_C Cuts at: 0 537 1051 1572 Size: 537 514 521 Fragments arranged by size: 537 521 514 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 1214 1572 Size: 463 751 358 Fragments arranged by size: 751 463 358 Bsp1286I G_dGCh'C Cuts at: 0 13 20 226 1448 1572 Size: 13 7 206 1222 124 Fragments arranged by size: 1222 206 124 13 7 BspHI T'CATG_A Cuts at: 0 737 1572 Size: 737 835 BsrFI r'CCGG_y Cuts at: 0 1325 1457 1572 Size: 1325 132 115 Fragments arranged by size: 1325 132 115 BsrGI T'GTAC_A Cuts at: 0 1550 1572 Size: 1550 22 BssSI C'ACGA_G Cuts at: 0 467 702 1471 1572 Size: 467 235 769 101 Fragments arranged by size: 769 467 235 101 BstYI r'GATC_y Cuts at: 0 507 1572 Size: 507 1065 BtsI GCAGTG_nn' Cuts at: 0 174 579 1202 1287 1572 Size: 174 405 623 85 285 Fragments arranged by size: 623 405 285 174 85 DraI TTT'AAA Cuts at: 0 1070 1482 1572 Size: 1070 412 90 Fragments arranged by size: 1070 412 90 EarI CTCTTCn'nnn_ Cuts at: 0 46 352 517 814 868 938 1211 1528 Size: 46 306 165 297 54 70 273 317 Cuts at: 1528 1572 Size: 44 Fragments arranged by size: 317 306 297 273 165 70 54 46 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 83 208 376 478 700 783 1067 1417 Size: 83 125 168 102 222 83 284 350 Cuts at: 1417 1513 1572 Size: 96 59 Fragments arranged by size: 350 284 222 168 125 102 96 83 83 59 EcoO109I rG'GnC_Cy Cuts at: 0 1005 1312 1572 Size: 1005 307 260 Fragments arranged by size: 1005 307 260 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 307 1434 1572 Size: 307 1127 138 Fragments arranged by size: 1127 307 138 HaeII r_GCGC'y Cuts at: 0 88 98 1572 Size: 88 10 1474 Fragments arranged by size: 1474 88 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 714 746 921 953 1169 1201 1539 1571 Size: 714 32 175 32 216 32 338 32 Cuts at: 1571 1572 Size: 1 Fragments arranged by size: 714 338 216 175 32 32 32 32 1 HincII GTy'rAC Cuts at: 0 236 905 1572 Size: 236 669 667 Fragments arranged by size: 669 667 236 MslI CAynn'nnrTG Cuts at: 0 485 1279 1572 Size: 485 794 293 Fragments arranged by size: 794 485 293 MspA1I CmG'CkG Cuts at: 0 153 1572 Size: 153 1419 NdeI CA'TA_TG Cuts at: 0 1164 1572 Size: 1164 408 NspI r_CATG'y Cuts at: 0 882 1323 1572 Size: 882 441 249 Fragments arranged by size: 882 441 249 PfoI T'CCnGG_A Cuts at: 0 494 1572 Size: 494 1078 PpuMI rG'GwC_Cy Cuts at: 0 1005 1312 1572 Size: 1005 307 260 Fragments arranged by size: 1005 307 260 PspOMI G'GGCC_C Cuts at: 0 9 1572 Size: 9 1563 PstI C_TGCA'G Cuts at: 0 52 158 215 1572 Size: 52 106 57 1357 Fragments arranged by size: 1357 106 57 52 PvuII CAG'CTG Cuts at: 0 153 1572 Size: 153 1419 SapI GCTCTTCn'nnn_ Cuts at: 0 46 868 1528 1572 Size: 46 822 660 44 Fragments arranged by size: 822 660 46 44 SfcI C'TryA_G Cuts at: 0 48 154 211 1572 Size: 48 106 57 1361 Fragments arranged by size: 1361 106 57 48 SmlI C'TyrA_G Cuts at: 0 836 1572 Size: 836 736 StyI C'CwwG_G Cuts at: 0 1000 1025 1572 Size: 1000 25 547 Fragments arranged by size: 1000 547 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 797 1572 Size: 797 775 TatI w'GTAC_w Cuts at: 0 859 1550 1572 Size: 859 691 22 Fragments arranged by size: 859 691 22 XbaI T'CTAG_A Cuts at: 0 255 1572 Size: 255 1317 XmnI GAAnn'nnTTC Cuts at: 0 315 1498 1572 Size: 315 1183 74 Fragments arranged by size: 1183 315 74 Enzymes that do cut: AcuI AlfI AlwNI ApaI ApoI BanII BbsI BciVI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI Bsp1286I BspHI BsrFI BsrGI BssSI BstYI BtsI DraI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII MslI MspA1I NdeI NspI PfoI PpuMI PspOMI PstI PvuII SapI SfcI SmlI StyI TaqII TatI XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI XmaI ZraI