##################################
# NO CUT ENZYMES (  226 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     KspI        MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AfaI           86  GTAC                 GT^AC
AluI          126  AGCT                 AG^CT
BfaI          128  CTAG                 C^TAG
BsaJI           8  CCTTGG               C^C[ACGT][ACGT]GG
BsmAI          32  GTCTC                GTCTC(1/5)
BsmBI          31  CGTCTC               CGTCTC(1/5)
BsmFI         198  GTCCC                GGGAC(10/14)
BsmI           47  GCATTC               GAATGC(1/-1)
BspHI          99  TCATGA               T^CATGA
BstBI          41  TTCGAA               TT^CGAA
BstXI         220  CCAAAGGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           20  GCGC                 GCG^C
EcoT14I         8  CCTTGG               C^C[AT][AT]GG
HhaI           20  GCGC                 GCG^C
HinP1I         20  GCGC                 G^CGC
HinfI         234  GAATC                G^A[ACGT]TC
MaeI          128  CTAG                 C^TAG
MaeII          84  ACGT                 A^CGT
MnlI          231  GAGG                 CCTC(7/6)
NdeI          161  CATATG               CA^TATG
NspV           41  TTCGAA               TT^CGAA
RcaI           99  TCATGA               T^CATGA
RsaI           86  GTAC                 GT^AC
SfuI           41  TTCGAA               TT^CGAA
StyI            8  CCTTGG               C^C[AT][AT]GG
TaqI           42  TCGA                 T^CGA
TfiI          234  GAATC                G^A[AT]TC
TthHB8I        42  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

MaeIII        151  GTTAC                ^GT[ACGT]AC
              214  GTTAC               
MwoI          118  GCTGTATTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              127  GCTAGTGGAGC         
NlaIII        100  CATG                 CATG^
              155  CATG                
NlaIV         133  GGAGCC               GG[ACGT]^[ACGT]CC
              191  GGAACC              


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################