(Circular) (Six-base) MAPSORT of: pFN21AE5716.seq Check: 1931 from: 1 to: 6526 pFN21AE5716 6526 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3387 3641 3387 Size: 254 6272 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2401 4336 278 Size: 53 83 186 936 865 1935 2468 Fragments arranged by size: 2468 1935 936 865 186 83 53 AccI GT'mk_AC Cuts at: 1937 3869 1937 Size: 1932 4594 Acc65I G'GTAC_C Cuts at: 3847 3847 Size: 6526 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4589 5973 6241 1632 Size: 2957 1384 268 1917 Fragments arranged by size: 2957 1917 1384 268 AfeI AGC'GCT Cuts at: 3068 3068 Size: 6526 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 5425 6402 1203 Size: 4222 977 1327 Fragments arranged by size: 4222 1327 977 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 2164 5255 2164 Size: 3091 3435 AleI CACnn'nnGTG Cuts at: 1166 3332 1166 Size: 2166 4360 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6526 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 2442 Size: 32 6494 AlwNI CAG_nnn'CTG Cuts at: 2215 2875 3286 3370 3395 3693 4269 5841 2215 Size: 660 411 84 25 298 576 1572 2900 Fragments arranged by size: 2900 1572 660 576 411 298 84 25 ApaI G_GGCC'C Cuts at: 2857 2857 Size: 6526 ApaLI G'TGCA_C Cuts at: 4583 5739 4583 Size: 1156 5370 ApoI r'AATT_y Cuts at: 1493 2611 3354 3835 4061 4213 6322 1493 Size: 1118 743 481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 743 481 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2304 2778 2850 2940 3178 3851 1870 Size: 73 361 474 72 90 238 673 4545 Fragments arranged by size: 4545 673 474 361 238 90 73 72 AvrII C'CTAG_G Cuts at: 3269 3269 Size: 6526 BamHI G'GATC_C Cuts at: 2800 3856 4223 2800 Size: 1056 367 5103 Fragments arranged by size: 5103 1056 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3587 3847 5302 618 Size: 325 217 627 1800 260 1455 1842 Fragments arranged by size: 1842 1800 1455 627 325 260 217 BanII G_rGCy'C Cuts at: 1391 2857 3324 3845 1391 Size: 1466 467 521 4072 Fragments arranged by size: 4072 1466 521 467 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5555 BbvCI CC'TCA_GC Cuts at: 3396 3406 3545 3396 Size: 10 139 6377 Fragments arranged by size: 6377 139 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4717 4751 1903 Size: 34 50 34 2696 34 3678 Fragments arranged by size: 3678 2696 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5628 4418 Size: 1210 5316 BclI T'GATC_A Cuts at: 1548 3132 1548 Size: 1584 4942 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3482 3786 5137 Size: 107 122 71 631 605 1810 304 1351 Cuts at: 5137 136 Size: 1525 Fragments arranged by size: 1810 1525 1351 631 605 304 122 107 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 3683 3943 3683 Size: 260 6266 Bme1580I G_kGCm'C Cuts at: 1790 2857 4587 5743 1790 Size: 1067 1730 1156 2573 Fragments arranged by size: 2573 1730 1156 1067 BmgBI CAC'GTC Cuts at: 1318 2755 1318 Size: 1437 5089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4257 5215 442 Size: 416 53 83 399 24 2840 958 1753 Fragments arranged by size: 2840 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3421 3421 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 2549 2664 3154 3753 5186 1239 Size: 844 466 115 490 599 1433 2579 Fragments arranged by size: 2579 1433 844 599 490 466 115 Bpu10I CC'TnA_GC Cuts at: 3396 3406 3545 3396 Size: 10 139 6377 Fragments arranged by size: 6377 139 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2935 4651 5516 5814 2935 Size: 1716 865 298 3647 Fragments arranged by size: 3647 1716 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3602 3746 5189 882 Size: 617 2103 144 1443 2219 Fragments arranged by size: 2219 2103 1443 617 144 BsaAI yAC'GTr Cuts at: 493 1204 4317 493 Size: 711 3113 2702 Fragments arranged by size: 3113 2702 711 BsaBI GATnn'nnATC Cuts at: 6520 6520 Size: 6526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2398 4333 4715 5350 Size: 53 83 186 936 865 1935 382 635 Cuts at: 5350 275 Size: 1451 Fragments arranged by size: 1935 1451 936 865 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4959 5357 5631 5778 6189 4959 Size: 398 274 147 411 5296 Fragments arranged by size: 5296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3332 3362 3751 3781 1178 Size: 30 165 30 1929 30 389 30 3923 Fragments arranged by size: 3923 1929 389 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2302 2485 2996 2999 3008 3674 3677 Size: 1105 11 183 511 3 9 666 3 Cuts at: 3677 3680 3713 3716 3725 3746 3764 1186 Size: 3 33 3 9 21 18 3948 Fragments arranged by size: 3948 1105 666 511 183 33 21 18 11 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2215 2745 2951 3004 3808 5729 6225 2215 Size: 530 206 53 804 1921 496 2516 Fragments arranged by size: 2516 1921 804 530 496 206 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3383 1163 Size: 573 1647 4306 Fragments arranged by size: 4306 1647 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 3976 4737 5765 1540 Size: 275 186 1540 435 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 435 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 4587 4672 5743 2523 Size: 2064 85 1071 3306 Fragments arranged by size: 3306 2064 1071 85 BsmI GAATG_Cn' Cuts at: 4037 4130 4037 Size: 93 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2857 3324 3845 4587 4672 5743 Size: 399 733 334 467 521 742 85 1071 Cuts at: 5743 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 733 521 467 399 334 85 BspEI T'CCGG_A Cuts at: 6189 6189 Size: 6526 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3271 3387 3641 3881 844 Size: 2427 116 254 240 3489 Fragments arranged by size: 3489 2427 254 240 116 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2131 4413 1394 Size: 429 245 63 2282 3507 Fragments arranged by size: 3507 2282 429 245 63 BsrDI GCAATG_nn' Cuts at: 65 1248 3045 5021 5195 65 Size: 1183 1797 1976 174 1396 Fragments arranged by size: 1976 1797 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2323 5170 5357 1700 Size: 110 513 2847 187 2869 Fragments arranged by size: 2869 2847 513 187 110 BsrGI T'GTAC_A Cuts at: 96 3498 96 Size: 3402 3124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 2223 4586 5598 2223 Size: 2363 1012 3151 Fragments arranged by size: 3151 2363 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3370 794 Size: 2576 3950 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 3085 1184 Size: 1901 4625 BstXI CCAn_nnnn'nTGG Cuts at: 2899 3619 2899 Size: 720 5806 BstYI r'GATC_y Cuts at: 1972 2686 2800 3294 3312 3856 4223 4608 4625 Size: 714 114 494 18 544 367 385 17 Cuts at: 4625 6077 6163 6175 6521 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 714 544 494 385 367 346 114 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 3612 513 Size: 552 2547 3427 Fragments arranged by size: 3427 2547 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5787 Fragments arranged by size: 5787 683 56 BtsI GCAGTG_nn' Cuts at: 3327 3537 4042 4836 4856 3327 Size: 210 505 794 20 4997 Fragments arranged by size: 4997 794 505 210 20 ClaI AT'CG_AT Cuts at: 1554 2242 1554 Size: 688 5838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6491 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5533 809 Size: 507 4217 1802 Fragments arranged by size: 4217 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3538 3787 3973 Size: 54 1425 50 275 18 1708 249 186 Cuts at: 3973 4862 6491 8 Size: 889 1629 43 Fragments arranged by size: 1708 1629 1425 889 275 249 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 3973 1537 Size: 275 1726 435 4090 Fragments arranged by size: 4090 1726 435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2816 2985 3267 3666 3672 4456 1353 Size: 713 750 169 282 399 6 784 3423 Fragments arranged by size: 3423 784 750 713 399 282 169 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3166 5113 5487 5633 387 Size: 1064 138 1577 1947 374 146 1280 Fragments arranged by size: 1947 1577 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2549 2664 3154 3753 4589 5186 Size: 393 451 466 115 490 599 836 597 Cuts at: 5186 5973 6241 1239 Size: 787 268 1524 Fragments arranged by size: 1524 836 787 599 597 490 466 451 393 268 115 EcoO109I rG'GnC_Cy Cuts at: 2782 2854 3367 3400 2782 Size: 72 513 33 5908 Fragments arranged by size: 5908 513 72 33 EcoRI G'AATT_C Cuts at: 3354 3835 4213 3354 Size: 481 378 5667 Fragments arranged by size: 5667 481 378 EcoRV GAT'ATC Cuts at: 2593 2593 Size: 6526 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2486 2486 Size: 6526 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 3070 5673 1678 Size: 1392 2603 2531 Fragments arranged by size: 2603 2531 1392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 5167 5199 5241 Size: 32 135 32 578 32 2985 32 42 Cuts at: 5241 5273 1373 Size: 32 2626 Fragments arranged by size: 2985 2626 578 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3870 4116 669 Size: 988 281 1932 246 3079 Fragments arranged by size: 3079 1932 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KpnI G_GTAC'C Cuts at: 3851 3851 Size: 6526 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2322 2620 2807 5640 5824 1305 Size: 825 192 298 187 2833 184 2007 Fragments arranged by size: 2833 2007 825 298 192 187 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4704 Fragments arranged by size: 4704 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 3332 4484 4843 5002 518 Size: 648 1731 435 1152 359 159 2042 Fragments arranged by size: 2042 1731 1152 648 435 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2545 2922 3410 3942 4619 5767 6012 Size: 512 427 377 488 532 677 1148 245 Cuts at: 6012 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 532 512 488 427 377 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5974 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 3884 5429 1132 Size: 97 2655 1545 2229 Fragments arranged by size: 2655 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2857 1348 Size: 1509 5017 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6526 PfoI T'CCnGG_A Cuts at: 2355 3315 2355 Size: 960 5566 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2782 3400 2782 Size: 618 5908 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PspOMI G'GGCC_C Cuts at: 2853 2853 Size: 6526 PstI C_TGCA'G Cuts at: 830 3878 830 Size: 3048 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 PvuII CAG'CTG Cuts at: 2545 2922 2545 Size: 377 6149 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SanDI GG'GwC_CC Cuts at: 2782 2782 Size: 6526 SapI GCTCTTCn'nnn_ Cuts at: 3672 3672 Size: 6526 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2318 3571 3874 5009 5690 5881 826 Size: 219 1273 1253 303 1135 681 191 1471 Fragments arranged by size: 1471 1273 1253 1135 681 303 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 2306 3180 3853 1872 Size: 434 874 673 4545 Fragments arranged by size: 4545 874 673 434 SmlI C'TyrA_G Cuts at: 820 1017 1943 2914 4630 5531 5793 820 Size: 197 926 971 1716 901 262 1553 Fragments arranged by size: 1716 1553 971 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 3246 1517 Size: 1729 4797 StyI C'CwwG_G Cuts at: 513 1065 2405 2424 3269 3477 3965 513 Size: 552 1340 19 845 208 488 3074 Fragments arranged by size: 3074 1340 845 552 488 208 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 3402 3800 4553 4570 4723 4908 Size: 1255 582 1240 398 753 17 153 185 Cuts at: 4908 5353 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1240 753 582 445 398 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3498 4772 6484 Size: 274 80 33 51 1443 1521 1274 1712 Cuts at: 6484 96 Size: 138 Fragments arranged by size: 1712 1521 1443 1274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2758 2790 6486 6518 2758 Size: 32 3696 32 2766 Fragments arranged by size: 3696 2766 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2573 1316 Size: 1257 5269 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2468 3097 3619 2468 Size: 629 522 5375 Fragments arranged by size: 5375 629 522 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6526 XmaI C'CCGG_G Cuts at: 1870 2304 3178 3851 1870 Size: 434 874 673 4545 Fragments arranged by size: 4545 874 673 434 XmnI GAAnn'nnTTC Cuts at: 2021 4655 2021 Size: 2634 3892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2399 4334 276 Size: 53 83 186 936 865 1935 2468 Fragments arranged by size: 2468 1935 936 865 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI