################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BsaAI BsaI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 721 GTCTAC GT^[AC][GT]AC AcyI 1072 GGCGTC G[GA]^CG[CT]C AgeI 9 ACCGGT A^CCGGT AocI 498 CCTCAGG CC^T[ACGT]AGG ApaI 659 GGGCCC GGGCC^C AspI 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 690 GGTGCC G^G[CT][GA]CC BglII 95 AGATCT A^GATCT BlpI 875 GCTCAGC GC^T[ACGT]AGC BpmI 643 CTGGAG CTGGAG(16/14) Bpu1102I 875 GCTCAGC GC^T[ACGT]AGC BsaBI 127 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1072 GGCGTC G[GA]^CG[CT]C BsaWI 9 ACCGGT [AT]^CCGG[AT] BsgI 619 GTGCAG GTGCAG(16/14) BsiEI 1066 CGGCCG CG[GA][CT]^CG BspMI 728 ACCTGC ACCTGC(4/8) BsrBI 873 CCGCTC CCGCTC(-3/-3) BstYI 95 AGATCT [GA]^GATC[CT] Bsu36I 498 CCTCAGG CC^T[ACGT]AGG CelII 875 GCTCAGC GC^T[ACGT]AGC EagI 1066 CGGCCG C^GGCCG EclXI 1066 CGGCCG C^GGCCG Eco52I 1066 CGGCCG C^GGCCG Eco81I 498 CCTCAGG CC^T[ACGT]AGG HaeII 503 GGCGCT [GA]GCGC^[CT] Hin1I 1072 GGCGTC G[GA]^CG[CT]C HindIII 1174 AAGCTT A^AGCTT MaeII 314 ACGT A^CGT MamI 127 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 95 AGATCT [GA]^GATC[CT] NaeI 693 GCCGGC GCC^GGC NgoMIV 693 GCCGGC G^CCGGC PflFI 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 237 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 9 ACCGGT A^CCGGT PpuMI 759 AGGTCCC [GA]G^G[AT]CC[CT] PstI 730 CTGCAG CTGCA^G SfcI 730 CTGCAG C^T[GA][CT]AG SmlI 136 CTCAAG C^T[CT][GA]AG TaqI 1058 TCGA T^CGA Tth111I 715 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1058 TCGA T^CGA Van91I 237 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 149 CCAATGCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 95 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 561 GTAC GT^AC 702 GTAC AviII 593 TGCGCA TGC^GCA 653 TGCGCA BalI 221 TGGCCA TGG^CCA 902 TGGCCA FspI 593 TGCGCA TGC^GCA 653 TGCGCA HgaI 359 GCGTC GACGC(5/10) 1073 GCGTC MluNI 221 TGGCCA TGG^CCA 902 TGGCCA MscI 221 TGGCCA TGG^CCA 902 TGGCCA NcoI 224 CCATGG C^CATGG 1156 CCATGG RsaI 561 GTAC GT^AC 702 GTAC SphI 446 GCATGC GCATG^C 1123 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AvaII BanII BbsI BbvI BcnI BglI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI