(Circular) (Six-base) MAPSORT of: pFN21AE4375.seq Check: 2997 from: 1 to: 5890 pFN21AE4375 5890 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2742 2742 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 3233 1937 Size: 790 506 4594 Fragments arranged by size: 4594 790 506 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 3953 5337 5605 1632 Size: 1058 1263 1384 268 1917 Fragments arranged by size: 1917 1384 1263 1058 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 2014 4721 2014 Size: 2707 3183 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 2870 1166 Size: 1704 4186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2216 2950 1184 Size: 1032 734 4124 Fragments arranged by size: 4124 1032 734 AlwNI CAG_nnn'CTG Cuts at: 3633 5205 3633 Size: 1572 4318 ApaI G_GGCC'C Cuts at: 2668 2668 Size: 5890 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 3199 3425 3577 5686 1493 Size: 1706 226 152 2109 1697 Fragments arranged by size: 2109 1706 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2695 3211 4666 618 Size: 325 217 627 908 516 1455 1842 Fragments arranged by size: 1842 1455 908 627 516 325 217 BanII G_rGCy'C Cuts at: 1391 2668 2833 2934 3209 1391 Size: 1277 165 101 275 4072 Fragments arranged by size: 4072 1277 275 165 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2047 2643 2724 3029 3128 928 Size: 971 148 596 81 305 99 3690 Fragments arranged by size: 3690 971 596 305 148 99 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 2495 2746 Size: 107 122 71 631 605 493 330 251 Cuts at: 2746 4501 136 Size: 1755 1525 Fragments arranged by size: 1755 1525 631 605 493 330 251 122 107 71 BglII A'GATC_T Cuts at: 2100 5885 2100 Size: 3785 2105 BlpI GC'TnA_GC Cuts at: 2881 3307 2881 Size: 426 5464 Bme1580I G_kGCm'C Cuts at: 1790 2668 3951 5107 1790 Size: 878 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 878 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2353 2736 3621 Size: 416 53 83 399 24 936 383 885 Cuts at: 3621 4579 442 Size: 958 1753 Fragments arranged by size: 1753 958 936 885 416 399 383 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 4550 1239 Size: 1430 1881 2579 Fragments arranged by size: 2579 1881 1430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 4015 4880 5178 2126 Size: 1889 865 298 2838 Fragments arranged by size: 2838 1889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4553 882 Size: 617 3054 2219 Fragments arranged by size: 3054 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 2136 5884 2136 Size: 3748 2142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3078 3697 4079 4714 Size: 53 83 186 936 1545 619 382 635 Cuts at: 4714 275 Size: 1451 Fragments arranged by size: 1545 1451 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2014 4323 4721 4995 5142 5553 2014 Size: 2309 398 274 147 411 2351 Fragments arranged by size: 2351 2309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5665 Fragments arranged by size: 5665 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2459 2510 2666 1186 Size: 1213 60 51 156 4410 Fragments arranged by size: 4410 1213 156 60 51 BseYI C'CCAG_C Cuts at: 2823 2901 5093 5589 2823 Size: 78 2192 496 3124 Fragments arranged by size: 3124 2192 496 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2645 1163 Size: 573 909 4408 Fragments arranged by size: 4408 909 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3074 3340 4101 5129 1540 Size: 275 186 1073 266 761 1028 2301 Fragments arranged by size: 2301 1073 1028 761 275 266 186 BsiHKAI G_wGCw'C Cuts at: 3951 4036 5107 3951 Size: 85 1071 4734 Fragments arranged by size: 4734 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2668 2833 2934 3209 3951 4036 5107 Size: 399 878 165 101 275 742 85 1071 Cuts at: 5107 1391 Size: 2174 Fragments arranged by size: 2174 1071 878 742 399 275 165 101 85 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2742 3245 844 Size: 1898 503 3489 Fragments arranged by size: 3489 1898 503 BsrBI CCG'CTC Cuts at: 1394 1823 2880 3777 1394 Size: 429 1057 897 3507 Fragments arranged by size: 3507 1057 897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2189 2698 4534 4721 1700 Size: 110 204 175 509 1836 187 2869 Fragments arranged by size: 2869 1836 509 204 187 175 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 3950 4962 3950 Size: 1012 4878 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5890 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2710 2917 2974 2710 Size: 207 57 5626 Fragments arranged by size: 5626 207 57 BstYI r'GATC_y Cuts at: 1972 2100 3220 3587 3972 3989 5441 5527 5539 Size: 128 1120 367 385 17 1452 86 12 Cuts at: 5539 5885 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1120 385 367 346 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2504 2504 Size: 5890 BtgI C'CryG_G Cuts at: 513 1065 2229 2691 3161 513 Size: 552 1164 462 470 3242 Fragments arranged by size: 3242 1164 552 470 462 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 2130 3406 4200 4220 2130 Size: 1276 794 20 3800 Fragments arranged by size: 3800 1276 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 2700 2791 Size: 54 1425 50 275 18 396 474 91 Cuts at: 2791 2907 2976 3071 3136 3337 4226 5855 8 Size: 116 69 95 65 201 889 1629 43 Fragments arranged by size: 1629 1425 889 474 396 275 201 116 95 91 69 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3071 3337 1537 Size: 275 1259 266 4090 Fragments arranged by size: 4090 1259 275 266 EarI CTCTTCn'nnn_ Cuts at: 1353 3820 1353 Size: 2467 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2669 2690 3953 4550 5337 5605 1239 Size: 393 1037 21 1263 597 787 268 1524 Fragments arranged by size: 1524 1263 1037 787 597 393 268 21 EcoO109I rG'GnC_Cy Cuts at: 2664 2665 2765 2844 2664 Size: 1 100 79 5710 Fragments arranged by size: 5710 100 79 1 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 FspI TGC'GCA Cuts at: 2600 2660 4396 2600 Size: 60 1736 4094 Fragments arranged by size: 4094 1736 60 FspAI rTGC'GCAy Cuts at: 2600 2600 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 2512 5037 1678 Size: 834 2525 2531 Fragments arranged by size: 2531 2525 834 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 2934 2966 2972 Size: 32 135 32 755 32 575 32 6 Cuts at: 2972 3004 4531 4563 4605 4637 1373 Size: 32 1527 32 42 32 2626 Fragments arranged by size: 2626 1527 755 575 135 42 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 3179 748 Size: 2431 3459 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5004 5188 1305 Size: 3699 184 2007 Fragments arranged by size: 3699 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2228 2909 10 Size: 54 1425 343 396 681 2991 Fragments arranged by size: 2991 1425 681 396 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2594 2621 2838 2870 2972 3848 Size: 648 1056 372 27 217 32 102 876 Cuts at: 3848 4207 4366 518 Size: 359 159 2042 Fragments arranged by size: 2042 1056 876 648 372 359 217 159 102 32 27 MspA1I CmG'CkG Cuts at: 1606 2245 2901 2986 3306 3983 5131 5376 1606 Size: 639 656 85 320 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 656 639 320 245 85 NaeI GCC'GGC Cuts at: 2700 2700 Size: 5890 NcoI C'CATG_G Cuts at: 513 1065 2229 3161 513 Size: 552 1164 932 3242 Fragments arranged by size: 3242 1164 932 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NgoMIV G'CCGG_C Cuts at: 2698 2698 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 2455 2572 3132 3248 4793 1132 Size: 97 1226 117 560 116 1545 2229 Fragments arranged by size: 2229 1545 1226 560 117 116 97 PasI CC'CwG_GG Cuts at: 1348 2770 1348 Size: 1422 4468 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 1108 Size: 1140 4750 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2765 2765 Size: 5890 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PspOMI G'GGCC_C Cuts at: 2664 2664 Size: 5890 PstI C_TGCA'G Cuts at: 830 2739 3242 830 Size: 1909 503 3478 Fragments arranged by size: 3478 1909 503 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2735 3238 4373 5054 5245 826 Size: 219 1690 503 1135 681 191 1471 Fragments arranged by size: 1690 1471 1135 681 503 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 3994 4895 5157 820 Size: 197 926 198 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 198 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 2455 3132 3248 1132 Size: 1323 677 116 3774 Fragments arranged by size: 3774 1323 677 116 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 2160 2229 2292 2868 3161 3329 513 Size: 552 1095 69 63 576 293 168 3074 Fragments arranged by size: 3074 1095 576 552 293 168 69 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 3917 3934 4087 4272 4717 325 Size: 1255 1313 1024 17 153 185 445 1498 Fragments arranged by size: 1498 1313 1255 1024 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2565 4136 5848 Size: 274 80 33 51 1443 588 1571 1712 Cuts at: 5848 96 Size: 138 Fragments arranged by size: 1712 1571 1443 588 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 5850 5882 2012 Size: 32 3806 32 2020 Fragments arranged by size: 3806 2020 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2723 1316 Size: 1407 4483 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2161 2161 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI