(Linear) (Six-base) MAPSORT of: KIEE4375.seq Check: 2970 from: 1 to: 1185 KIEE4375.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 737 1185 Size: 737 448 AccI GT'mk_AC Cuts at: 0 722 1185 Size: 722 463 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 1185 Size: 685 500 AgeI A'CCGG_T Cuts at: 0 9 1185 Size: 9 1176 AleI CACnn'nnGTG Cuts at: 0 865 1185 Size: 865 320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 211 945 1185 Size: 211 734 240 Fragments arranged by size: 734 240 211 ApaI G_GGCC'C Cuts at: 0 663 1185 Size: 663 522 BanI G'GyrC_C Cuts at: 0 690 1185 Size: 690 495 BanII G_rGCy'C Cuts at: 0 663 828 929 1185 Size: 663 165 101 256 Fragments arranged by size: 663 256 165 101 BbsI GAAGACnn'nnnn_ Cuts at: 0 42 638 719 1024 1123 1185 Size: 42 596 81 305 99 62 Fragments arranged by size: 596 305 99 81 62 42 BglI GCCn_nnn'nGGC Cuts at: 0 160 490 741 1185 Size: 160 330 251 444 Fragments arranged by size: 444 330 251 160 BglII A'GATC_T Cuts at: 0 95 1185 Size: 95 1090 BlpI GC'TnA_GC Cuts at: 0 876 1185 Size: 876 309 Bme1580I G_kGCm'C Cuts at: 0 663 1185 Size: 663 522 BmrI ACTGGGnnnn_n' Cuts at: 0 348 731 1185 Size: 348 383 454 Fragments arranged by size: 454 383 348 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 231 1185 Size: 231 954 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 664 1185 Size: 664 521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1185 Size: 121 1064 BsaBI GATnn'nnATC Cuts at: 0 131 1185 Size: 131 1054 BsaHI Gr'CG_yC Cuts at: 0 1073 1185 Size: 1073 112 BsaWI w'CCGG_w Cuts at: 0 9 1185 Size: 9 1176 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 394 454 505 661 1185 Size: 394 60 51 156 524 Fragments arranged by size: 524 394 156 60 51 BseYI C'CCAG_C Cuts at: 0 818 896 1185 Size: 818 78 289 Fragments arranged by size: 818 289 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1185 Size: 640 545 BsiEI CG_ry'CG Cuts at: 0 1069 1185 Size: 1069 116 Bsp1286I G_dGCh'C Cuts at: 0 663 828 929 1185 Size: 663 165 101 256 Fragments arranged by size: 663 256 165 101 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 737 1185 Size: 737 448 BsrBI CCG'CTC Cuts at: 0 875 1185 Size: 875 310 BsrFI r'CCGG_y Cuts at: 0 9 184 693 1185 Size: 9 175 509 492 Fragments arranged by size: 509 492 175 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 705 912 969 1185 Size: 705 207 57 216 Fragments arranged by size: 705 216 207 57 BstYI r'GATC_y Cuts at: 0 95 1185 Size: 95 1090 Bsu36I CC'TnA_GG Cuts at: 0 499 1185 Size: 499 686 BtgI C'CryG_G Cuts at: 0 224 686 1156 1185 Size: 224 462 470 29 Fragments arranged by size: 470 462 224 29 BtsI GCAGTG_nn' Cuts at: 0 125 1185 Size: 125 1060 EaeI y'GGCC_r Cuts at: 0 221 695 786 902 971 1066 1131 1185 Size: 221 474 91 116 69 95 65 54 Fragments arranged by size: 474 221 116 95 91 69 65 54 EagI C'GGCC_G Cuts at: 0 1066 1185 Size: 1066 119 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 664 685 1185 Size: 664 21 500 Fragments arranged by size: 664 500 21 EcoO109I rG'GnC_Cy Cuts at: 0 659 660 760 839 1185 Size: 659 1 100 79 346 Fragments arranged by size: 659 346 100 79 1 FspI TGC'GCA Cuts at: 0 595 655 1185 Size: 595 60 530 Fragments arranged by size: 595 530 60 FspAI rTGC'GCAy Cuts at: 0 595 1185 Size: 595 590 HaeII r_GCGC'y Cuts at: 0 507 1185 Size: 507 678 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 322 354 929 961 967 999 1185 Size: 322 32 575 32 6 32 186 Fragments arranged by size: 575 322 186 32 32 32 6 HindIII A'AGCT_T Cuts at: 0 1174 1185 Size: 1174 11 MscI TGG'CCA Cuts at: 0 223 904 1185 Size: 223 681 281 Fragments arranged by size: 681 281 223 MslI CAynn'nnrTG Cuts at: 0 217 589 616 833 865 967 1185 Size: 217 372 27 217 32 102 218 Fragments arranged by size: 372 218 217 217 102 32 27 MspA1I CmG'CkG Cuts at: 0 240 896 981 1185 Size: 240 656 85 204 Fragments arranged by size: 656 240 204 85 NaeI GCC'GGC Cuts at: 0 695 1185 Size: 695 490 NcoI C'CATG_G Cuts at: 0 224 1156 1185 Size: 224 932 29 Fragments arranged by size: 932 224 29 NgoMIV G'CCGG_C Cuts at: 0 693 1185 Size: 693 492 NspI r_CATG'y Cuts at: 0 450 567 1127 1185 Size: 450 117 560 58 Fragments arranged by size: 560 450 117 58 PasI CC'CwG_GG Cuts at: 0 765 1185 Size: 765 420 PflMI CCAn_nnn'nTGG Cuts at: 0 243 1185 Size: 243 942 PpuMI rG'GwC_Cy Cuts at: 0 760 1185 Size: 760 425 PspOMI G'GGCC_C Cuts at: 0 659 1185 Size: 659 526 PstI C_TGCA'G Cuts at: 0 734 1185 Size: 734 451 SfcI C'TryA_G Cuts at: 0 730 1185 Size: 730 455 SmlI C'TyrA_G Cuts at: 0 136 1185 Size: 136 1049 SphI G_CATG'C Cuts at: 0 450 1127 1185 Size: 450 677 58 Fragments arranged by size: 677 450 58 StyI C'CwwG_G Cuts at: 0 155 224 287 863 1156 1185 Size: 155 69 63 576 293 29 Fragments arranged by size: 576 293 155 69 63 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 888 1185 Size: 888 297 TatI w'GTAC_w Cuts at: 0 560 1185 Size: 560 625 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 7 39 1185 Size: 7 32 1146 Fragments arranged by size: 1146 32 7 Tth111I GACn'n_nGTC Cuts at: 0 718 1185 Size: 718 467 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 156 1185 Size: 156 1029 Enzymes that do cut: AarI AccI AcuI AgeI AleI AlfI ApaI BanI BanII BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI Eco57MI EcoO109I FspI FspAI HaeII Hin4I HindIII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PpuMI PspOMI PstI SfcI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI Bpu10I BsaI BsaAI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI ZraI