Restriction map for
pFN21AE2765
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbrPI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI ScaI SfcI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 1001 TCCGGA T^CCGGA
AcyI 513 GGCGCC G[GA]^CG[CT]C
AhdI 955 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 943 GTGCAC G^TGCAC
AlwNI 247 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 943 GTGCAC G^TGCAC
AspEI 955 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 490 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 441 CTCGGG C^[CT]CG[GA]G
BalI 98 TGGCCA TGG^CCA
BanII 133 GAGCTC G[GA]GC[CT]^C
BbeI 513 GGCGCC GGCGC^C
BbsI 459 GTCTTC GAAGAC(2/6)
BbvCI 303 GCTGAGG CCTCAGC(-5/-2)
BclI 158 TGATCA T^GATCA
BglI 976 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 459 GTCTTC GAAGAC(2/6)
BsaHI 513 GGCGCC G[GA]^CG[CT]C
BsaWI 1001 TCCGGA [AT]^CCGG[AT]
BseAI 1001 TCCGGA T^CCGGA
BsgI 180 CTGCAC GTGCAG(16/14)
BsmAI 153 GAGAC GTCTC(1/5)
BsmFI 544 GGGAC GGGAC(10/14)
BsoBI 441 CTCGGG C^[CT]CG[GA]G
BspEI 1001 TCCGGA T^CCGGA
BspMI 178 ACCTGC ACCTGC(4/8)
BssSI 43 CTCGTG CACGAG(-5/-1)
BstEII 3 GGTGACC G^GT[ACGT]ACC
BstPI 3 GGTGACC G^GT[ACGT]ACC
DraII 19 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 955 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 19 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3 GGTGACC G^GT[ACGT]ACC
EcoT22I 832 ATGCAT ATGCA^T
FbaI 158 TGATCA T^GATCA
Hin1I 513 GGCGCC G[GA]^CG[CT]C
KasI 513 GGCGCC G^GCGCC
KspI 917 CCGCGG CCGC^GG
MaeII 968 ACGT A^CGT
MluNI 98 TGGCCA TGG^CCA
MroI 1001 TCCGGA T^CCGGA
MscI 98 TGGCCA TGG^CCA
MslI 90 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 416 GCCGGC GCC^GGC
NarI 513 GGCGCC GG^CGCC
NcoI 92 CCATGG C^CATGG
NgoMIV 416 GCCGGC G^CCGGC
NsiI 832 ATGCAT ATGCA^T
NspI 834 GCATGT [GA]CATG^[CT]
PflFI 490 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 19 AGGACCC [GA]G^G[AT]CC[CT]
SacI 133 GAGCTC GAGCT^C
SacII 917 CCGCGG CCGC^GG
SapI 48 GCTCTTC GCTCTTC(1/4)
SexAI 106 ACCTGGT A^CC[AT]GGT
SfiI 975 GGCCACCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 513 GGCGCC GGC^GCC
StuI 169 AGGCCT AGG^CCT
TspRI 187 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 490 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 554 CCACCCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 270 CGCG CG^CG
918 CGCG
AfaI 570 GTAC GT^AC
858 GTAC
AocI 450 CCTGAGG CC^T[ACGT]AGG
498 CCTCAGG
BcnI 585 CCGGG CC^[GC]GG
693 CCCGG
BsaBI 729 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
794 GATACCCATC
BsrFI 416 GCCGGC [GA]^CCGG[CT]
805 ACCGGC
BstAPI 182 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
246 GCAGCCGCTGC
BstUI 270 CGCG CG^CG
918 CGCG
BstXI 116 CCAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
632 CCATCGACTTGG
BstYI 728 AGATCC [GA]^GATC[CT]
1004 GGATCT
Bsu36I 450 CCTGAGG CC^T[ACGT]AGG
498 CCTCAGG
Cac8I 203 GCAAGC GC[ACGT]^[ACGT]GC
416 GCCGGC
Cfr10I 416 GCCGGC [GA]^CCGG[CT]
805 ACCGGC
EaeI 98 TGGCCA [CT]^GGCC[GA]
974 CGGCCA
Eco81I 450 CCTGAGG CC^T[ACGT]AGG
498 CCTCAGG
EcoT14I 92 CCATGG C^C[AT][AT]GG
980 CCTTGG
FauI 315 GCGGG CCCGC(4/6)
919 GCGGG
HinfI 457 GAGTC G^A[ACGT]TC
961 GAGTC
MamI 729 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
794 GATACCCATC
MflI 728 AGATCC [GA]^GATC[CT]
1004 GGATCT
MvnI 270 CGCG CG^CG
918 CGCG
NciI 585 CCGGG CC^[GC]GG
693 CCCGG
PleI 457 GAGTC GAGTC(4/5)
961 GAGTC
RsaI 570 GTAC GT^AC
858 GTAC
StyI 92 CCATGG C^C[AT][AT]GG
980 CCTTGG
TaqI 221 TCGA T^CGA
635 TCGA
TthHB8I 221 TCGA T^CGA
635 TCGA
XhoII 728 AGATCC [GA]^GATC[CT]
1004 GGATCT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I Van91I