(Circular) (Six-base) MAPSORT of: pFN21AE2765.seq Check: 6068 from: 1 to: 5725 pFN21AE2765 5725 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2192 2192 Size: 5725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3068 1937 Size: 1131 4594 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 3788 5172 5440 1632 Size: 542 1614 1384 268 1917 Fragments arranged by size: 1917 1614 1384 542 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 4624 5601 1203 Size: 3421 977 1327 Fragments arranged by size: 3421 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 2965 4454 2965 Size: 1489 4236 AleI CACnn'nnGTG Cuts at: 1166 2099 1166 Size: 933 4792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2903 2935 2903 Size: 32 5693 AlwNI CAG_nnn'CTG Cuts at: 2257 3468 5040 2257 Size: 1211 1572 2942 Fragments arranged by size: 2942 1572 1211 ApaLI G'TGCA_C Cuts at: 2948 3782 4938 2948 Size: 834 1156 3735 Fragments arranged by size: 3735 1156 834 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2446 3050 1870 Size: 73 503 604 4545 Fragments arranged by size: 4545 604 503 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2374 2518 2623 2675 2895 Size: 325 217 627 587 144 105 52 220 Cuts at: 2895 3046 4501 618 Size: 151 1455 1842 Fragments arranged by size: 1842 1455 627 587 325 220 217 151 144 105 52 BanII G_rGCy'C Cuts at: 1391 2142 3044 1391 Size: 751 902 4072 Fragments arranged by size: 4072 902 751 BbeI G_GCGC'C Cuts at: 2522 2522 Size: 5725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2457 928 Size: 971 558 4196 Fragments arranged by size: 4196 971 558 BbvCI CC'TCA_GC Cuts at: 2309 2309 Size: 5725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 2163 1548 Size: 615 5110 BfrBI ATG'CAT Cuts at: 2839 2839 Size: 5725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2987 4336 136 Size: 107 122 71 631 605 1315 1349 1525 Fragments arranged by size: 1525 1349 1315 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2628 2678 2793 2952 3786 4942 1790 Size: 838 50 115 159 834 1156 2573 Fragments arranged by size: 2573 1156 838 834 159 115 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2914 3456 4414 Size: 416 53 83 399 24 1497 542 958 Cuts at: 4414 442 Size: 1753 Fragments arranged by size: 1753 1497 958 542 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2300 2420 4385 1239 Size: 917 144 120 1965 2579 Fragments arranged by size: 2579 1965 917 144 120 Bpu10I CC'TnA_GC Cuts at: 2309 2309 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4715 5013 3850 Size: 865 298 4562 Fragments arranged by size: 4562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 2738 2803 5719 2738 Size: 65 2916 2744 Fragments arranged by size: 2916 2744 65 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2519 3532 3914 4549 Size: 53 83 186 936 986 1013 382 635 Cuts at: 4549 275 Size: 1451 Fragments arranged by size: 1451 1013 986 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3006 4158 4556 4830 4977 5388 3006 Size: 1152 398 274 147 411 3343 Fragments arranged by size: 3343 1152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 2592 2622 1178 Size: 30 165 30 990 30 169 30 4281 Fragments arranged by size: 4281 990 169 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5725 BseYI C'CCAG_C Cuts at: 2596 2749 4928 5424 2596 Size: 153 2179 496 2897 Fragments arranged by size: 2897 2179 496 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2170 1163 Size: 573 434 4718 Fragments arranged by size: 4718 573 434 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 2055 2142 2952 3786 3871 4942 2040 Size: 15 87 810 834 85 1071 2823 Fragments arranged by size: 2823 1071 834 810 87 85 15 BsmI GAATG_Cn' Cuts at: 3236 3329 3236 Size: 93 5632 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2055 2142 2628 2678 2793 2952 Size: 399 250 15 87 486 50 115 159 Cuts at: 2952 3044 3786 3871 4942 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 399 250 159 115 92 87 85 50 15 BspEI T'CCGG_A Cuts at: 3006 5388 3006 Size: 2382 3343 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2192 3080 844 Size: 1348 888 3489 Fragments arranged by size: 3489 1348 888 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2421 2810 4369 4556 1700 Size: 110 611 389 1559 187 2869 Fragments arranged by size: 2869 1559 611 389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 2048 3785 4797 2048 Size: 1737 1012 2976 Fragments arranged by size: 2976 1737 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 2257 794 Size: 1399 64 4262 Fragments arranged by size: 4262 1399 64 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 2008 1184 Size: 824 4901 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2644 2128 Size: 516 5209 BstYI r'GATC_y Cuts at: 1972 2733 3009 3055 3422 3807 3824 5276 5362 Size: 761 276 46 367 385 17 1452 86 Cuts at: 5362 5374 5720 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 761 385 367 346 276 86 46 17 12 Bsu36I CC'TnA_GG Cuts at: 2456 2504 2456 Size: 48 5677 BtgI C'CryG_G Cuts at: 513 1065 2004 2097 2523 2670 2922 513 Size: 552 939 93 426 147 252 3316 Fragments arranged by size: 3316 939 552 426 252 147 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2862 487 Size: 683 56 1636 3350 Fragments arranged by size: 3350 1636 683 56 BtsI GCAGTG_nn' Cuts at: 2191 3241 4035 4055 2191 Size: 1050 794 20 3861 Fragments arranged by size: 3861 1050 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2979 3172 Size: 54 1425 50 275 18 273 876 193 Cuts at: 3172 4061 5690 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 876 275 273 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2060 2150 2399 3655 1353 Size: 662 45 90 249 1256 3423 Fragments arranged by size: 3423 1256 662 249 90 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2140 2140 Size: 5725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2174 2300 2420 3788 4385 5172 Size: 393 524 18 126 120 1368 597 787 Cuts at: 5172 5440 1239 Size: 268 1524 Fragments arranged by size: 1524 1368 787 597 524 393 268 126 120 18 EcoO109I rG'GnC_Cy Cuts at: 2025 2025 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2785 2785 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2428 2522 2664 4872 1678 Size: 750 94 142 2208 2531 Fragments arranged by size: 2531 2208 750 142 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2425 2473 2505 2592 Size: 32 135 32 821 32 48 32 87 Cuts at: 2592 2624 2951 2983 4366 4398 4440 4472 1373 Size: 32 327 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 821 327 135 87 48 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KasI G'GCGC_C Cuts at: 2518 2518 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4839 5023 1305 Size: 3534 184 2007 Fragments arranged by size: 3534 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 10 Size: 54 1425 343 273 3630 Fragments arranged by size: 3630 1425 343 273 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 3683 4042 4201 518 Size: 648 933 1584 359 159 2042 Fragments arranged by size: 2042 1584 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 2320 2924 3141 3818 4966 5211 1606 Size: 651 63 604 217 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 604 245 217 63 NaeI GCC'GGC Cuts at: 2423 2423 Size: 5725 NarI GG'CG_CC Cuts at: 2519 2519 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 2004 2097 513 Size: 552 939 93 4141 Fragments arranged by size: 4141 939 552 93 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NgoMIV G'CCGG_C Cuts at: 2421 2421 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NsiI A_TGCA'T Cuts at: 2841 2841 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2843 3083 4628 1132 Size: 97 1614 240 1545 2229 Fragments arranged by size: 2229 1614 1545 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2098 2112 2569 1108 Size: 990 14 457 4264 Fragments arranged by size: 4264 990 457 14 PfoI T'CCnGG_A Cuts at: 2133 2589 2133 Size: 456 5269 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2025 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 SacI G_AGCT'C Cuts at: 2142 2142 Size: 5725 SacII CC_GC'GG Cuts at: 2925 2925 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 5725 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SexAI A'CCwGG_T Cuts at: 2111 2111 Size: 5725 SfcI C'TryA_G Cuts at: 826 1045 3073 4208 4889 5080 826 Size: 219 2028 1135 681 191 1471 Fragments arranged by size: 2028 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2987 2987 Size: 5725 SfoI GGC'GCC Cuts at: 2520 2520 Size: 5725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3829 4730 4992 820 Size: 197 926 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 3083 1132 Size: 1951 3774 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 2176 1517 Size: 659 5066 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2985 3164 513 Size: 552 939 93 888 179 3074 Fragments arranged by size: 3074 939 888 552 179 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2576 2641 2763 2812 3752 3769 Size: 1255 624 372 65 122 49 940 17 Cuts at: 3769 3922 4107 4552 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 940 624 445 372 185 153 122 65 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5685 5717 5685 Size: 32 5693 Tth111I GACn'n_nGTC Cuts at: 1316 2498 1316 Size: 1182 4543 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2566 2566 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SrfI SwaI