################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbrPI BbvCI BciVI BglII BsaAI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI Ksp632I MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tsp509I TspRI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 1273 GTCGAC GT^[AC][GT]AC AfaI 1378 GTAC GT^AC AflII 903 CTTAAG C^TTAAG Alw44I 1318 GTGCAC G^TGCAC Aor51HI 804 AGCGCT AGC^GCT ApaI 29 GGGCCC GGGCC^C ApaLI 1318 GTGCAC G^TGCAC AvrII 783 CCTAGG C^CTAGG BamHI 1056 GGATCC G^GATCC BbsI 721 GTCTTC GAAGAC(2/6) BcgI 1072 CGAGCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 425 TGATCA T^GATCA BfaI 784 CTAG C^TAG BfrI 903 CTTAAG C^TTAAG BlnI 783 CCTAGG C^CTAGG BpuAI 721 GTCTTC GAAGAC(2/6) BsaBI 225 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 259 GAGACC GGTCTC(1/5) BsgI 1201 CTGCAC GTGCAG(16/14) BsiEI 1386 CGACCG CG[GA][CT]^CG BsmBI 131 GAGACG CGTCTC(1/5) BsmI 345 GCATTC GAATGC(1/-1) BspMI 390 ACCTGC ACCTGC(4/8) BsrFI 1103 GCCGGC [GA]^CCGG[CT] BstEII 915 GGTCACC G^GT[ACGT]ACC BstPI 915 GGTCACC G^GT[ACGT]ACC Cfr10I 1103 GCCGGC [GA]^CCGG[CT] Eco47III 804 AGCGCT AGC^GCT EcoNI 1362 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 915 GGTCACC G^GT[ACGT]ACC FbaI 425 TGATCA T^GATCA HgaI 759 GACGC GACGC(5/10) HincII 1273 GTCGAC GT[CT]^[GA]AC HindII 1273 GTCGAC GT[CT]^[GA]AC KspI 992 CCGCGG CCGC^GG MaeI 784 CTAG C^TAG MamI 225 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 722 TCTTC GAAGA(8/7) MslI 219 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1103 GCCGGC GCC^GGC NcoI 815 CCATGG C^CATGG NgoMIV 1103 GCCGGC G^CCGGC NspI 403 GCATGT [GA]CATG^[CT] PstI 1363 CTGCAG CTGCA^G RsaI 1378 GTAC GT^AC SacII 992 CCGCGG CCGC^GG SalI 1273 GTCGAC G^TCGAC SbfI 1362 CCTGCAGG CCTGCA^GG SexAI 911 ACCTGGT A^CC[AT]GGT SfcI 1363 CTGCAG C^T[GA][CT]AG SmlI 903 CTTAAG C^T[CT][GA]AG Sse8387I 1362 CCTGCAGG CCTGCA^GG StuI 317 AGGCCT AGG^CCT XcmI 350 CCACAGGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### BalI 227 TGGCCA TGG^CCA 310 TGGCCA BglI 394 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 982 GCCCGGGTGGC BlpI 945 GCTCAGC GC^T[ACGT]AGC 1338 GCTCAGC Bpu1102I 945 GCTCAGC GC^T[ACGT]AGC 1338 GCTCAGC BsmAI 131 GAGAC GTCTC(1/5) 259 GAGAC BssHII 194 GCGCGC G^CGCGC 1391 GCGCGC BssSI 1204 CACGAG CACGAG(-5/-1) 667 CTCGTG BstAPI 519 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1107 GCAGCTGCTGC BstXI 218 CCATGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 313 CCAGAGGCCTGG BstYI 249 GGATCT [GA]^GATC[CT] 1056 GGATCC CelII 945 GCTCAGC GC^T[ACGT]AGC 1338 GCTCAGC MflI 249 GGATCT [GA]^GATC[CT] 1056 GGATCC MluNI 227 TGGCCA TGG^CCA 310 TGGCCA MscI 227 TGGCCA TGG^CCA 310 TGGCCA MseI 701 TTAA T^TAA 904 TTAA PflMI 276 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 846 CCACGCCCTGG PleI 963 GACTC GAGTC(4/5) 475 GAGTC PpuMI 908 GGGACCT [GA]G^G[AT]CC[CT] 1300 GGGACCC SacI 1207 GAGCTC GAGCT^C 1423 GAGCTC SfaNI 516 GATGC GCATC(5/9) 1035 GATGC TaqI 1028 TCGA T^CGA 1274 TCGA TfiI 488 GATTC G^A[AT]TC 587 GAATC Tru9I 701 TTAA T^TAA 904 TTAA TthHB8I 1028 TCGA T^CGA 1274 TCGA Van91I 276 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 846 CCACGCCCTGG XhoII 249 GGATCT [GA]^GATC[CT] 1056 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AspHI AspI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflFI PvuII Sau3AI Sau96I ScrFI SfoI SmaI StyI TseI Tsp45I Tth111I XmaCI XmaI