(Circular) (Six-base) MAPSORT of: pFN21AE1421.seq Check: 8821 from: 1 to: 6151 pFN21AE1421 6151 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2404 2404 Size: 6151 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3279 3494 1937 Size: 1342 215 4594 Fragments arranged by size: 4594 1342 215 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4214 5598 5866 1632 Size: 2582 1384 268 1917 Fragments arranged by size: 2582 1917 1384 268 AfeI AGC'GCT Cuts at: 2811 2811 Size: 6151 AflII C'TTAA_G Cuts at: 820 1017 2908 820 Size: 197 1891 4063 Fragments arranged by size: 4063 1891 197 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2431 1184 Size: 1247 4904 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2693 2725 2693 Size: 32 6119 AlwNI CAG_nnn'CTG Cuts at: 2287 2308 3118 3295 3894 5466 2287 Size: 21 810 177 599 1572 2972 Fragments arranged by size: 2972 1572 810 599 177 21 ApaI G_GGCC'C Cuts at: 2038 2038 Size: 6151 ApaLI G'TGCA_C Cuts at: 3323 4208 5364 3323 Size: 885 1156 4110 Fragments arranged by size: 4110 1156 885 ApoI r'AATT_y Cuts at: 1493 3460 3686 3838 5947 1493 Size: 1967 226 152 2109 1697 Fragments arranged by size: 2109 1967 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2748 2930 2988 3075 3088 3129 Size: 73 80 725 182 58 87 13 41 Cuts at: 3129 3215 3476 1870 Size: 86 261 4545 Fragments arranged by size: 4545 725 261 182 87 86 80 73 58 41 13 AvrII C'CTAG_G Cuts at: 2788 2788 Size: 6151 BamHI G'GATC_C Cuts at: 3061 3481 3848 3061 Size: 420 367 5364 Fragments arranged by size: 5364 420 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2328 2422 2462 2936 3151 Size: 325 217 627 541 94 40 474 215 Cuts at: 3151 3164 3472 4927 618 Size: 13 308 1455 1842 Fragments arranged by size: 1842 1455 627 541 474 325 308 217 215 94 40 13 BanII G_rGCy'C Cuts at: 1391 2038 2103 2513 3082 3216 3432 3470 1391 Size: 647 65 410 569 134 216 38 4072 Fragments arranged by size: 4072 647 569 410 216 134 65 38 BbeI G_GCGC'C Cuts at: 2332 2940 3155 2332 Size: 608 215 5328 Fragments arranged by size: 5328 608 215 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2719 928 Size: 971 820 4360 Fragments arranged by size: 4360 971 820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3066 3100 4342 4376 1903 Size: 34 1129 34 1242 34 3678 Fragments arranged by size: 3678 1242 1129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 5269 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2405 2993 4762 Size: 107 122 71 631 605 733 588 1769 Cuts at: 4762 136 Size: 1525 Fragments arranged by size: 1769 1525 733 631 605 588 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 2951 3344 3568 2951 Size: 393 224 5534 Fragments arranged by size: 5534 393 224 Bme1580I G_kGCm'C Cuts at: 1790 2038 2454 3327 4212 5368 1790 Size: 248 416 873 885 1156 2573 Fragments arranged by size: 2573 1156 885 873 416 248 BmgBI CAC'GTC Cuts at: 1318 2441 3283 1318 Size: 1123 842 4186 Fragments arranged by size: 4186 1123 842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3882 4840 442 Size: 416 53 83 399 24 2465 958 1753 Fragments arranged by size: 2465 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2284 2284 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2330 2498 2879 4811 1239 Size: 1040 51 168 381 1932 2579 Fragments arranged by size: 2579 1932 1040 381 168 51 Bpu10I CC'TnA_GC Cuts at: 2150 2976 2150 Size: 826 5325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4276 5141 5439 4276 Size: 865 298 4988 Fragments arranged by size: 4988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4814 882 Size: 617 759 2556 2219 Fragments arranged by size: 2556 2219 759 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 2234 6145 2234 Size: 3911 2240 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2329 2937 3152 3958 Size: 53 83 186 936 796 608 215 806 Cuts at: 3958 4340 4975 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 806 796 635 608 382 215 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2669 2699 1178 Size: 30 165 30 1266 30 4630 Fragments arranged by size: 4630 1266 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2882 3155 1186 Size: 1090 606 273 4182 Fragments arranged by size: 4182 1090 606 273 BseYI C'CCAG_C Cuts at: 3236 5354 5850 3236 Size: 2118 496 3537 Fragments arranged by size: 3537 2118 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3191 1163 Size: 573 1455 4123 Fragments arranged by size: 4123 1455 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 3601 4362 5390 1540 Size: 275 186 1393 207 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 207 186 BsiHKAI G_wGCw'C Cuts at: 3089 3216 3327 3432 4212 4297 5368 3089 Size: 127 111 105 780 85 1071 3872 Fragments arranged by size: 3872 1071 780 127 111 105 85 BsmI GAATG_Cn' Cuts at: 2350 3662 3755 2350 Size: 1312 93 4746 Fragments arranged by size: 4746 1312 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2130 2130 Size: 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2103 2454 2513 3082 3089 3216 Size: 399 248 65 351 59 569 7 127 Cuts at: 3216 3327 3432 3470 4212 4297 5368 1391 Size: 111 105 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 399 351 248 127 111 105 85 65 59 38 7 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2404 3506 844 Size: 1560 1102 3489 Fragments arranged by size: 3489 1560 1102 BsrBI CCG'CTC Cuts at: 1394 1823 2690 3037 3247 4038 1394 Size: 429 867 347 210 791 3507 Fragments arranged by size: 3507 867 791 429 347 210 BsrDI GCAATG_nn' Cuts at: 65 1248 4646 4820 65 Size: 1183 3398 174 1396 Fragments arranged by size: 3398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3108 4795 4982 1700 Size: 110 1298 1687 187 2869 Fragments arranged by size: 2869 1687 1298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 2199 3396 1925 Size: 274 1197 4680 Fragments arranged by size: 4680 1197 274 BssSI C'ACGA_G Cuts at: 2672 3209 4211 5223 2672 Size: 537 1002 1012 3600 Fragments arranged by size: 3600 1012 1002 537 BstAPI GCAn_nnn'nTGC Cuts at: 794 2530 3118 794 Size: 1736 588 3827 Fragments arranged by size: 3827 1736 588 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 2920 1184 Size: 1736 4415 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2325 2230 Size: 95 6056 BstYI r'GATC_y Cuts at: 1972 2254 3061 3481 3848 4233 4250 5702 5788 Size: 282 807 420 367 385 17 1452 86 Cuts at: 5788 5800 6146 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 807 420 385 367 346 282 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2625 2820 2997 3168 3301 513 Size: 552 939 621 195 177 171 133 3363 Fragments arranged by size: 3363 939 621 552 195 177 171 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 3667 4461 4481 3667 Size: 794 20 5337 Fragments arranged by size: 5337 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2232 2315 Size: 54 1425 50 275 18 348 54 83 Cuts at: 2315 2994 3025 3598 4487 6116 8 Size: 679 31 573 889 1629 43 Fragments arranged by size: 1629 1425 889 679 573 348 275 83 54 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4081 1353 Size: 2728 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3239 4738 5112 5258 387 Size: 1064 138 1650 1499 374 146 1280 Fragments arranged by size: 1650 1499 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3214 3430 3214 Size: 216 5935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2330 2498 2879 4214 4811 5598 Size: 393 647 51 168 381 1335 597 787 Cuts at: 5598 5866 1239 Size: 268 1524 Fragments arranged by size: 1524 1335 787 647 597 393 381 268 168 51 EcoNI CCTnn'n_nnAGG Cuts at: 3371 3371 Size: 6151 EcoO109I rG'GnC_Cy Cuts at: 2034 2035 2284 2914 3306 2034 Size: 1 249 630 392 4879 Fragments arranged by size: 4879 630 392 249 1 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 2332 2813 2876 2940 2985 3005 3155 3188 Size: 654 481 63 64 45 20 150 33 Cuts at: 3188 3200 3380 5298 1678 Size: 12 180 1918 2531 Fragments arranged by size: 2531 1918 654 481 180 150 64 63 45 33 20 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2484 2516 2669 2701 2959 Size: 32 135 32 912 32 153 32 258 Cuts at: 2959 2991 2999 3031 4792 4824 4866 4898 1373 Size: 32 8 32 1761 32 42 32 2626 Fragments arranged by size: 2626 1761 912 258 153 135 42 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 3280 3495 3741 669 Size: 988 281 1342 215 246 3079 Fragments arranged by size: 3079 1342 988 281 246 215 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KasI G'GCGC_C Cuts at: 2328 2936 3151 2328 Size: 608 215 5328 Fragments arranged by size: 5328 608 215 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2263 3414 5265 5449 1305 Size: 958 1151 1851 184 2007 Fragments arranged by size: 2007 1851 1151 958 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 2317 10 Size: 54 1425 343 402 83 3844 Fragments arranged by size: 3844 1425 402 343 83 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 4109 4468 4627 518 Size: 648 1062 1881 359 159 2042 Fragments arranged by size: 2042 1881 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2183 2308 2436 2575 2955 2999 3115 3193 Size: 577 125 128 139 380 44 116 78 Cuts at: 3193 3348 3567 4244 5392 5637 1606 Size: 155 219 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 577 380 245 219 155 139 128 125 116 78 44 NaeI GCC'GGC Cuts at: 3110 3110 Size: 6151 NarI GG'CG_CC Cuts at: 2329 2937 3152 2329 Size: 608 215 5328 Fragments arranged by size: 5328 608 215 NcoI C'CATG_G Cuts at: 513 1065 2004 2820 513 Size: 552 939 816 3844 Fragments arranged by size: 3844 939 816 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NgoMIV G'CCGG_C Cuts at: 3108 3108 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2412 3509 5054 1132 Size: 97 1183 1097 1545 2229 Fragments arranged by size: 2229 1545 1183 1097 97 PasI CC'CwG_GG Cuts at: 1348 2833 1348 Size: 1485 4666 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2287 2857 1108 Size: 1179 570 4402 Fragments arranged by size: 4402 1179 570 PfoI T'CCnGG_A Cuts at: 2112 2488 2112 Size: 376 5775 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2914 3306 2914 Size: 392 5759 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PspOMI G'GGCC_C Cuts at: 2034 2034 Size: 6151 PstI C_TGCA'G Cuts at: 830 3372 3503 830 Size: 2542 131 3478 Fragments arranged by size: 3478 2542 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2308 2575 3115 2308 Size: 267 540 5344 Fragments arranged by size: 5344 540 267 SacI G_AGCT'C Cuts at: 3216 3432 3216 Size: 216 5935 SacII CC_GC'GG Cuts at: 3000 3000 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3278 3493 1936 Size: 1342 215 4594 Fragments arranged by size: 4594 1342 215 SanDI GG'GwC_CC Cuts at: 3306 3306 Size: 6151 SbfI CC_TGCA'GG Cuts at: 3372 3503 3372 Size: 131 6020 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SexAI A'CCwGG_T Cuts at: 2916 2916 Size: 6151 SfcI C'TryA_G Cuts at: 826 1045 3368 3499 4634 5315 5506 826 Size: 219 2323 131 1135 681 191 1471 Fragments arranged by size: 2323 1471 1135 681 219 191 131 SfoI GGC'GCC Cuts at: 2330 2938 3153 2330 Size: 608 215 5328 Fragments arranged by size: 5328 608 215 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 2025 2750 2990 3131 3478 1872 Size: 153 725 240 141 347 4545 Fragments arranged by size: 4545 725 347 240 153 141 SmlI C'TyrA_G Cuts at: 820 1017 1943 2908 4255 5156 5418 820 Size: 197 926 965 1347 901 262 1553 Fragments arranged by size: 1553 1347 965 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 2324 1517 Size: 807 5344 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 2788 2820 3022 3590 513 Size: 552 939 462 322 32 202 568 3074 Fragments arranged by size: 3074 939 568 552 462 322 202 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 4178 4195 4348 4533 4978 325 Size: 1255 777 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4397 6109 96 Size: 274 80 33 51 1443 2420 1712 138 Fragments arranged by size: 2420 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6111 6143 6111 Size: 32 6119 Tth111I GACn'n_nGTC Cuts at: 1316 2375 2442 2918 1316 Size: 1059 67 476 4549 Fragments arranged by size: 4549 1059 476 67 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 2362 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 2023 2748 2988 3129 3476 1870 Size: 153 725 240 141 347 4545 Fragments arranged by size: 4545 725 347 240 153 141 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI