(Linear) (Six-base) MAPSORT of: KIEE1421.seq Check: 5646 from: 1 to: 1446 KIEE1421.orf With 182 enzymes: SgfI * July 3, 2012 16:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 399 1446 Size: 399 1047 AccI GT'mk_AC Cuts at: 0 1274 1446 Size: 1274 172 AfeI AGC'GCT Cuts at: 0 806 1446 Size: 806 640 AflII C'TTAA_G Cuts at: 0 903 1446 Size: 903 543 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 426 1446 Size: 426 1020 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 688 720 1446 Size: 688 32 726 Fragments arranged by size: 726 688 32 AlwNI CAG_nnn'CTG Cuts at: 0 282 303 1113 1290 1446 Size: 282 21 810 177 156 Fragments arranged by size: 810 282 177 156 21 ApaI G_GGCC'C Cuts at: 0 33 1446 Size: 33 1413 ApaLI G'TGCA_C Cuts at: 0 1318 1446 Size: 1318 128 AvaI C'yCGr_G Cuts at: 0 18 743 925 983 1070 1083 1124 1210 Size: 18 725 182 58 87 13 41 86 Cuts at: 1210 1446 Size: 236 Fragments arranged by size: 725 236 182 87 86 58 41 18 13 AvrII C'CTAG_G Cuts at: 0 783 1446 Size: 783 663 BamHI G'GATC_C Cuts at: 0 1056 1446 Size: 1056 390 BanI G'GyrC_C Cuts at: 0 323 417 457 931 1146 1159 1446 Size: 323 94 40 474 215 13 287 Fragments arranged by size: 474 323 287 215 94 40 13 BanII G_rGCy'C Cuts at: 0 33 98 508 1077 1211 1427 1446 Size: 33 65 410 569 134 216 19 Fragments arranged by size: 569 410 216 134 65 33 19 BbeI G_GCGC'C Cuts at: 0 327 935 1150 1446 Size: 327 608 215 296 Fragments arranged by size: 608 327 296 215 BbsI GAAGACnn'nnnn_ Cuts at: 0 714 1446 Size: 714 732 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1061 1095 1446 Size: 1061 34 351 Fragments arranged by size: 1061 351 34 BclI T'GATC_A Cuts at: 0 425 1446 Size: 425 1021 BglI GCCn_nnn'nGGC Cuts at: 0 400 988 1446 Size: 400 588 458 Fragments arranged by size: 588 458 400 BlpI GC'TnA_GC Cuts at: 0 946 1339 1446 Size: 946 393 107 Fragments arranged by size: 946 393 107 Bme1580I G_kGCm'C Cuts at: 0 33 449 1322 1446 Size: 33 416 873 124 Fragments arranged by size: 873 416 124 33 BmgBI CAC'GTC Cuts at: 0 436 1278 1446 Size: 436 842 168 Fragments arranged by size: 842 436 168 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 279 1446 Size: 279 1167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 325 493 874 1446 Size: 274 51 168 381 572 Fragments arranged by size: 572 381 274 168 51 Bpu10I CC'TnA_GC Cuts at: 0 145 971 1446 Size: 145 826 475 Fragments arranged by size: 826 475 145 BsaI GGTCTCn'nnnn_ Cuts at: 0 253 1446 Size: 253 1193 BsaBI GATnn'nnATC Cuts at: 0 229 1446 Size: 229 1217 BsaHI Gr'CG_yC Cuts at: 0 324 932 1147 1446 Size: 324 608 215 299 Fragments arranged by size: 608 324 299 215 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 664 694 1446 Size: 664 30 752 Fragments arranged by size: 752 664 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 271 877 1150 1446 Size: 271 606 273 296 Fragments arranged by size: 606 296 273 271 BseYI C'CCAG_C Cuts at: 0 1231 1446 Size: 1231 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1186 1446 Size: 1186 260 BsiEI CG_ry'CG Cuts at: 0 1389 1446 Size: 1389 57 BsiHKAI G_wGCw'C Cuts at: 0 1084 1211 1322 1427 1446 Size: 1084 127 111 105 19 Fragments arranged by size: 1084 127 111 105 19 BsmI GAATG_Cn' Cuts at: 0 345 1446 Size: 345 1101 BsmBI CGTCTCn'nnnn_ Cuts at: 0 125 1446 Size: 125 1321 Bsp1286I G_dGCh'C Cuts at: 0 33 98 449 508 1077 1084 1211 1322 Size: 33 65 351 59 569 7 127 111 Cuts at: 1322 1427 1446 Size: 105 19 Fragments arranged by size: 569 351 127 111 105 65 59 33 19 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 399 1446 Size: 399 1047 BsrBI CCG'CTC Cuts at: 0 685 1032 1242 1446 Size: 685 347 210 204 Fragments arranged by size: 685 347 210 204 BsrFI r'CCGG_y Cuts at: 0 1103 1446 Size: 1103 343 BssHII G'CGCG_C Cuts at: 0 194 1391 1446 Size: 194 1197 55 Fragments arranged by size: 1197 194 55 BssSI C'ACGA_G Cuts at: 0 667 1204 1446 Size: 667 537 242 Fragments arranged by size: 667 537 242 BstAPI GCAn_nnn'nTGC Cuts at: 0 525 1113 1446 Size: 525 588 333 Fragments arranged by size: 588 525 333 BstEII G'GTnAC_C Cuts at: 0 915 1446 Size: 915 531 BstXI CCAn_nnnn'nTGG Cuts at: 0 225 320 1446 Size: 225 95 1126 Fragments arranged by size: 1126 225 95 BstYI r'GATC_y Cuts at: 0 249 1056 1446 Size: 249 807 390 Fragments arranged by size: 807 390 249 BtgI C'CryG_G Cuts at: 0 620 815 992 1163 1296 1446 Size: 620 195 177 171 133 150 Fragments arranged by size: 620 195 177 171 150 133 EaeI y'GGCC_r Cuts at: 0 173 227 310 989 1020 1446 Size: 173 54 83 679 31 426 Fragments arranged by size: 679 426 173 83 54 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1234 1446 Size: 1234 212 EcoICRI GAG'CTC Cuts at: 0 1209 1425 1446 Size: 1209 216 21 Fragments arranged by size: 1209 216 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 325 493 874 1446 Size: 274 51 168 381 572 Fragments arranged by size: 572 381 274 168 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 1366 1446 Size: 1366 80 EcoO109I rG'GnC_Cy Cuts at: 0 29 30 279 909 1301 1446 Size: 29 1 249 630 392 145 Fragments arranged by size: 630 392 249 145 29 1 HaeII r_GCGC'y Cuts at: 0 327 808 871 935 980 1000 1150 1183 Size: 327 481 63 64 45 20 150 33 Cuts at: 1183 1195 1375 1446 Size: 12 180 71 Fragments arranged by size: 481 327 180 150 71 64 63 45 33 20 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 479 511 664 696 954 986 994 1026 Size: 479 32 153 32 258 32 8 32 Cuts at: 1026 1446 Size: 420 Fragments arranged by size: 479 420 258 153 32 32 32 32 8 HincII GTy'rAC Cuts at: 0 1275 1446 Size: 1275 171 KasI G'GCGC_C Cuts at: 0 323 931 1146 1446 Size: 323 608 215 300 Fragments arranged by size: 608 323 300 215 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 258 1409 1446 Size: 258 1151 37 Fragments arranged by size: 1151 258 37 MscI TGG'CCA Cuts at: 0 229 312 1446 Size: 229 83 1134 Fragments arranged by size: 1134 229 83 MslI CAynn'nnrTG Cuts at: 0 223 1446 Size: 223 1223 MspA1I CmG'CkG Cuts at: 0 178 303 431 570 950 994 1110 1188 Size: 178 125 128 139 380 44 116 78 Cuts at: 1188 1343 1446 Size: 155 103 Fragments arranged by size: 380 178 155 139 128 125 116 103 78 44 NaeI GCC'GGC Cuts at: 0 1105 1446 Size: 1105 341 NarI GG'CG_CC Cuts at: 0 324 932 1147 1446 Size: 324 608 215 299 Fragments arranged by size: 608 324 299 215 NcoI C'CATG_G Cuts at: 0 815 1446 Size: 815 631 NgoMIV G'CCGG_C Cuts at: 0 1103 1446 Size: 1103 343 NspI r_CATG'y Cuts at: 0 407 1446 Size: 407 1039 PasI CC'CwG_GG Cuts at: 0 828 1446 Size: 828 618 PflMI CCAn_nnn'nTGG Cuts at: 0 282 852 1446 Size: 282 570 594 Fragments arranged by size: 594 570 282 PfoI T'CCnGG_A Cuts at: 0 107 483 1446 Size: 107 376 963 Fragments arranged by size: 963 376 107 PpuMI rG'GwC_Cy Cuts at: 0 909 1301 1446 Size: 909 392 145 Fragments arranged by size: 909 392 145 PspOMI G'GGCC_C Cuts at: 0 29 1446 Size: 29 1417 PstI C_TGCA'G Cuts at: 0 1367 1446 Size: 1367 79 PvuII CAG'CTG Cuts at: 0 303 570 1110 1446 Size: 303 267 540 336 Fragments arranged by size: 540 336 303 267 SacI G_AGCT'C Cuts at: 0 1211 1427 1446 Size: 1211 216 19 Fragments arranged by size: 1211 216 19 SacII CC_GC'GG Cuts at: 0 995 1446 Size: 995 451 SalI G'TCGA_C Cuts at: 0 1273 1446 Size: 1273 173 SanDI GG'GwC_CC Cuts at: 0 1301 1446 Size: 1301 145 SbfI CC_TGCA'GG Cuts at: 0 1367 1446 Size: 1367 79 SexAI A'CCwGG_T Cuts at: 0 911 1446 Size: 911 535 SfcI C'TryA_G Cuts at: 0 1363 1446 Size: 1363 83 SfoI GGC'GCC Cuts at: 0 325 933 1148 1446 Size: 325 608 215 298 Fragments arranged by size: 608 325 298 215 SmaI CCC'GGG Cuts at: 0 20 745 985 1126 1446 Size: 20 725 240 141 320 Fragments arranged by size: 725 320 240 141 20 SmlI C'TyrA_G Cuts at: 0 903 1446 Size: 903 543 StuI AGG'CCT Cuts at: 0 319 1446 Size: 319 1127 StyI C'CwwG_G Cuts at: 0 461 783 815 1017 1446 Size: 461 322 32 202 429 Fragments arranged by size: 461 429 322 202 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 352 1446 Size: 352 1094 Tth111I GACn'n_nGTC Cuts at: 0 370 437 913 1446 Size: 370 67 476 533 Fragments arranged by size: 533 476 370 67 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 357 1446 Size: 357 1089 XmaI C'CCGG_G Cuts at: 0 18 743 983 1124 1446 Size: 18 725 240 141 322 Fragments arranged by size: 725 322 240 141 18 Enzymes that do cut: AarI AccI AfeI AflII AlfI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SbfI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AflIII AgeI AhdI AleI ApoI AscI AseI AsiSI BaeI BbvCI BciVI BfrBI BglII BmrI BmtI BpuEI BsaAI BsaWI BsiWI BspEI BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmnI ZraI