(Circular) (Six-base) MAPSORT of: pFN21AE1471.seq Check: 8497 from: 1 to: 5413 pFN21AE1471 5413 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2756 1937 Size: 819 4594 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3476 4860 5128 1632 Size: 1844 1384 268 1917 Fragments arranged by size: 1917 1844 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 2277 4312 5289 1203 Size: 1074 2035 977 1327 Fragments arranged by size: 2035 1327 1074 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 2512 3156 4728 2512 Size: 644 1572 3197 Fragments arranged by size: 3197 1572 644 ApaI G_GGCC'C Cuts at: 2148 2148 Size: 5413 ApaLI G'TGCA_C Cuts at: 3470 4626 3470 Size: 1156 4257 ApoI r'AATT_y Cuts at: 1493 2722 2948 3100 5209 1493 Size: 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 226 152 AscI GG'CGCG_CC Cuts at: 2183 2183 Size: 5413 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2738 1870 Size: 73 795 4545 Fragments arranged by size: 4545 795 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2266 2299 2266 Size: 33 5380 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2551 2590 2596 2734 Size: 325 217 627 463 301 39 6 138 Cuts at: 2734 4189 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 463 325 301 217 138 39 6 BanII G_rGCy'C Cuts at: 1391 2055 2148 2406 2612 2732 1391 Size: 664 93 258 206 120 4072 Fragments arranged by size: 4072 664 258 206 120 93 BbeI G_GCGC'C Cuts at: 2594 2600 2594 Size: 6 5407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2588 928 Size: 971 689 3753 Fragments arranged by size: 3753 971 689 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 1903 Size: 34 1667 34 3678 Fragments arranged by size: 3678 1667 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4024 136 Size: 107 122 71 631 605 2352 1525 Fragments arranged by size: 2352 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5408 5408 Size: 5413 BlpI GC'TnA_GC Cuts at: 2830 2830 Size: 5413 Bme1580I G_kGCm'C Cuts at: 1790 2025 2148 2556 3474 4630 1790 Size: 235 123 408 918 1156 2573 Fragments arranged by size: 2573 1156 918 408 235 123 BmgBI CAC'GTC Cuts at: 1318 2278 1318 Size: 960 4453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2134 3144 4102 Size: 416 53 83 399 24 717 1010 958 Cuts at: 4102 442 Size: 1753 Fragments arranged by size: 1753 1010 958 717 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 2710 2350 Size: 360 5053 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 4073 1239 Size: 830 2004 2579 Fragments arranged by size: 2579 2004 830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 2174 3538 4403 4701 2039 Size: 135 1364 865 298 2751 Fragments arranged by size: 2751 1364 865 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2433 2591 2597 3220 Size: 53 83 186 936 900 158 6 623 Cuts at: 3220 3602 4237 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 900 635 623 382 186 158 83 53 6 BsaWI w'CCGG_w Cuts at: 2499 3846 4244 4518 4665 5076 2499 Size: 1347 398 274 147 411 2836 Fragments arranged by size: 2836 1347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5188 Fragments arranged by size: 5188 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 2672 2720 1186 Size: 1483 3 48 3879 Fragments arranged by size: 3879 1483 48 3 BseYI C'CCAG_C Cuts at: 2024 4616 5112 2024 Size: 2592 496 2325 Fragments arranged by size: 2592 2325 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4840 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 2079 2863 3624 4652 1540 Size: 275 186 14 64 784 761 1028 2301 Fragments arranged by size: 2301 1028 784 761 275 186 64 14 BsiHKAI G_wGCw'C Cuts at: 2055 3474 3559 4630 2055 Size: 1419 85 1071 2838 Fragments arranged by size: 2838 1419 1071 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 BsmBI CGTCTCn'nnnn_ Cuts at: 2261 2261 Size: 5413 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2055 2148 2406 2556 2612 2732 Size: 399 235 30 93 258 150 56 120 Cuts at: 2732 3474 3559 4630 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 258 235 150 120 93 85 56 30 BspEI T'CCGG_A Cuts at: 2499 5076 2499 Size: 2577 2836 BspHI T'CATG_A Cuts at: 3302 5032 3302 Size: 1730 3683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2497 2768 844 Size: 1653 271 3489 Fragments arranged by size: 3489 1653 271 BsrBI CCG'CTC Cuts at: 1394 1823 2322 3300 1394 Size: 429 499 978 3507 Fragments arranged by size: 3507 978 499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3908 4082 65 Size: 1183 2660 174 1396 Fragments arranged by size: 2660 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 2302 2593 4057 4244 1700 Size: 110 264 228 291 1464 187 2869 Fragments arranged by size: 2869 1464 291 264 228 187 110 BsrGI T'GTAC_A Cuts at: 96 2445 96 Size: 2349 3064 BssHII G'CGCG_C Cuts at: 1925 2183 1925 Size: 258 5155 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5413 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstXI CCAn_nnnn'nTGG Cuts at: 2563 2563 Size: 5413 BstYI r'GATC_y Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408 Size: 771 367 385 17 1452 86 12 346 Cuts at: 5408 1972 Size: 1977 Fragments arranged by size: 1977 1452 771 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3723 3743 2929 Size: 794 20 4599 Fragments arranged by size: 4599 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2717 2717 Size: 5413 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2133 2565 Size: 54 1425 50 275 18 182 121 432 Cuts at: 2565 2860 3749 5378 8 Size: 295 889 1629 43 Fragments arranged by size: 1629 1425 889 432 295 275 182 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2012 2860 1537 Size: 275 200 848 4090 Fragments arranged by size: 4090 848 275 200 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2624 3343 1353 Size: 711 560 719 3423 Fragments arranged by size: 3423 719 711 560 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2053 2053 Size: 5413 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 3476 4073 4860 5128 1239 Size: 393 437 1407 597 787 268 1524 Fragments arranged by size: 1524 1407 787 597 437 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2338 2338 Size: 5413 EcoO109I rG'GnC_Cy Cuts at: 2145 2509 2145 Size: 364 5049 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 2042 2594 2600 4560 1678 Size: 364 552 6 1960 2531 Fragments arranged by size: 2531 1960 552 364 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 4054 4086 4128 Size: 32 135 32 1022 32 1428 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 1022 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2757 3003 669 Size: 988 281 819 246 3079 Fragments arranged by size: 3079 988 819 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5413 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KasI G'GCGC_C Cuts at: 2590 2596 2590 Size: 6 5407 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4527 4711 1305 Size: 3222 184 2007 Fragments arranged by size: 3222 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 10 Size: 54 1425 343 303 3288 Fragments arranged by size: 3288 1425 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2670 2829 3506 4654 4899 1606 Size: 1064 159 677 1148 245 2120 Fragments arranged by size: 2120 1148 1064 677 245 159 NaeI GCC'GGC Cuts at: 2595 2595 Size: 5413 NarI GG'CG_CC Cuts at: 2591 2597 2591 Size: 6 5407 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NgoMIV G'CCGG_C Cuts at: 2593 2593 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2771 4316 1132 Size: 97 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 4312 4312 Size: 5413 PflMI CCAn_nnn'nTGG Cuts at: 1108 2247 1108 Size: 1139 4274 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2509 2509 Size: 5413 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5413 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PspOMI G'GGCC_C Cuts at: 2144 2144 Size: 5413 PstI C_TGCA'G Cuts at: 830 2067 2765 830 Size: 1237 698 3478 Fragments arranged by size: 3478 1237 698 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 PvuII CAG'CTG Cuts at: 2670 2670 Size: 5413 SacI G_AGCT'C Cuts at: 2055 2055 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SapI GCTCTTCn'nnn_ Cuts at: 2064 2064 Size: 5413 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 2573 3661 5373 2573 Size: 1088 1712 2613 Fragments arranged by size: 2613 1712 1088 SfcI C'TryA_G Cuts at: 826 1045 2063 2761 3896 4577 4768 826 Size: 219 1018 698 1135 681 191 1471 Fragments arranged by size: 1471 1135 1018 698 681 219 191 SfoI GGC'GCC Cuts at: 2592 2598 2592 Size: 6 5407 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 2302 2593 1700 Size: 602 291 4520 Fragments arranged by size: 4520 602 291 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 2189 3517 4418 4680 820 Size: 197 926 111 135 1328 901 262 1553 Fragments arranged by size: 1553 1328 926 901 262 197 135 111 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5413 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 3337 5 Size: 47 3285 2081 Fragments arranged by size: 3285 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 2586 2852 513 Size: 552 939 516 66 266 3074 Fragments arranged by size: 3074 939 552 516 266 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3440 3457 3610 3795 4240 325 Size: 1255 1860 17 153 185 445 1498 Fragments arranged by size: 1860 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 2484 2571 Size: 274 80 33 51 1443 468 39 87 Cuts at: 2571 3659 5371 96 Size: 1088 1712 138 Fragments arranged by size: 1712 1443 1088 468 274 138 87 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 5373 5405 2076 Size: 32 3265 32 2084 Fragments arranged by size: 3265 2084 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2749 2749 Size: 5413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 3542 3542 Size: 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI