(Linear) (Six-base) MAPSORT of: KIEE1471.seq Check: 2473 from: 1 to: 708 KIEE1471.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AflIII A'CryG_T Cuts at: 0 272 708 Size: 272 436 AlwNI CAG_nnn'CTG Cuts at: 0 507 708 Size: 507 201 ApaI G_GGCC'C Cuts at: 0 143 708 Size: 143 565 AscI GG'CGCG_CC Cuts at: 0 178 708 Size: 178 530 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 261 294 708 Size: 261 33 414 Fragments arranged by size: 414 261 33 BanI G'GyrC_C Cuts at: 0 245 546 585 591 708 Size: 245 301 39 6 117 Fragments arranged by size: 301 245 117 39 6 BanII G_rGCy'C Cuts at: 0 50 143 401 607 708 Size: 50 93 258 206 101 Fragments arranged by size: 258 206 101 93 50 BbeI G_GCGC'C Cuts at: 0 589 595 708 Size: 589 6 113 Fragments arranged by size: 589 113 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 583 708 Size: 583 125 Bme1580I G_kGCm'C Cuts at: 0 20 143 551 708 Size: 20 123 408 157 Fragments arranged by size: 408 157 123 20 BmgBI CAC'GTC Cuts at: 0 273 708 Size: 273 435 BmrI ACTGGGnnnn_n' Cuts at: 0 129 708 Size: 129 579 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 345 705 708 Size: 345 360 3 Fragments arranged by size: 360 345 3 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 708 Size: 64 644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 169 708 Size: 34 135 539 Fragments arranged by size: 539 135 34 BsaHI Gr'CG_yC Cuts at: 0 428 586 592 708 Size: 428 158 6 116 Fragments arranged by size: 428 158 116 6 BsaWI w'CCGG_w Cuts at: 0 494 708 Size: 494 214 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 664 667 708 Size: 664 3 41 Fragments arranged by size: 664 41 3 BseYI C'CCAG_C Cuts at: 0 19 708 Size: 19 689 BsiEI CG_ry'CG Cuts at: 0 10 74 708 Size: 10 64 634 Fragments arranged by size: 634 64 10 BsiHKAI G_wGCw'C Cuts at: 0 50 708 Size: 50 658 BsmBI CGTCTCn'nnnn_ Cuts at: 0 256 708 Size: 256 452 Bsp1286I G_dGCh'C Cuts at: 0 20 50 143 401 551 607 708 Size: 20 30 93 258 150 56 101 Fragments arranged by size: 258 150 101 93 56 30 20 BspEI T'CCGG_A Cuts at: 0 494 708 Size: 494 214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 492 708 Size: 492 216 BsrBI CCG'CTC Cuts at: 0 317 708 Size: 317 391 BsrFI r'CCGG_y Cuts at: 0 69 297 588 708 Size: 69 228 291 120 Fragments arranged by size: 291 228 120 69 BsrGI T'GTAC_A Cuts at: 0 440 708 Size: 440 268 BssHII G'CGCG_C Cuts at: 0 178 708 Size: 178 530 BstXI CCAn_nnnn'nTGG Cuts at: 0 558 708 Size: 558 150 EaeI y'GGCC_r Cuts at: 0 7 128 560 708 Size: 7 121 432 148 Fragments arranged by size: 432 148 121 7 EagI C'GGCC_G Cuts at: 0 7 708 Size: 7 701 EarI CTCTTCn'nnn_ Cuts at: 0 59 619 708 Size: 59 560 89 Fragments arranged by size: 560 89 59 EcoICRI GAG'CTC Cuts at: 0 48 708 Size: 48 660 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 708 Size: 64 644 EcoNI CCTnn'n_nnAGG Cuts at: 0 333 708 Size: 333 375 EcoO109I rG'GnC_Cy Cuts at: 0 140 504 708 Size: 140 364 204 Fragments arranged by size: 364 204 140 HaeII r_GCGC'y Cuts at: 0 37 589 595 708 Size: 37 552 6 113 Fragments arranged by size: 552 113 37 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 589 621 708 Size: 589 32 87 Fragments arranged by size: 589 87 32 KasI G'GCGC_C Cuts at: 0 585 591 708 Size: 585 6 117 Fragments arranged by size: 585 117 6 MscI TGG'CCA Cuts at: 0 130 708 Size: 130 578 MspA1I CmG'CkG Cuts at: 0 665 708 Size: 665 43 NaeI GCC'GGC Cuts at: 0 590 708 Size: 590 118 NarI GG'CG_CC Cuts at: 0 586 592 708 Size: 586 6 116 Fragments arranged by size: 586 116 6 NgoMIV G'CCGG_C Cuts at: 0 588 708 Size: 588 120 PflMI CCAn_nnn'nTGG Cuts at: 0 242 708 Size: 242 466 PpuMI rG'GwC_Cy Cuts at: 0 504 708 Size: 504 204 PspOMI G'GGCC_C Cuts at: 0 139 708 Size: 139 569 PstI C_TGCA'G Cuts at: 0 62 708 Size: 62 646 PvuII CAG'CTG Cuts at: 0 665 708 Size: 665 43 SacI G_AGCT'C Cuts at: 0 50 708 Size: 50 658 SapI GCTCTTCn'nnn_ Cuts at: 0 59 708 Size: 59 649 ScaI AGT'ACT Cuts at: 0 568 708 Size: 568 140 SfcI C'TryA_G Cuts at: 0 58 708 Size: 58 650 SfoI GGC'GCC Cuts at: 0 587 593 708 Size: 587 6 115 Fragments arranged by size: 587 115 6 SgrAI Cr'CCGG_yG Cuts at: 0 297 588 708 Size: 297 291 120 Fragments arranged by size: 297 291 120 SmlI C'TyrA_G Cuts at: 0 49 184 708 Size: 49 135 524 Fragments arranged by size: 524 135 49 StyI C'CwwG_G Cuts at: 0 515 581 708 Size: 515 66 127 Fragments arranged by size: 515 127 66 TatI w'GTAC_w Cuts at: 0 440 479 566 708 Size: 440 39 87 142 Fragments arranged by size: 440 142 87 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 71 103 708 Size: 71 32 605 Fragments arranged by size: 605 71 32 Enzymes that do cut: AflIII AlwNI ApaI AscI BaeI BanI BanII BbeI BbsI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BstXI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MspA1I NaeI NarI NgoMIV PflMI PpuMI PspOMI PstI PvuII SacI SapI ScaI SfcI SfoI SgrAI SmlI StyI TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI Bpu10I BsaI BsaAI BsaBI BsaXI BsgI BsiWI BsmI BspHI BsrDI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI