################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AfaI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 200 GTAGAC GT^[AC][GT]AC AciI 778 GCGG CCGC(-3/-1) AclI 810 AACGTT AA^CGTT AflIII 459 ACATGT A^C[GA][CT]GT AlwNI 223 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 562 CCTGAGG CC^T[ACGT]AGG AvaI 485 CTCGGG C^[CT]CG[GA]G AvaII 955 GGTCC G^G[AT]CC AvrII 534 CCTAGG C^CTAGG BalI 1372 TGGCCA TGG^CCA BamHI 757 GGATCC G^GATCC BanI 529 GGTGCC G^G[CT][GA]CC BlnI 534 CCTAGG C^CTAGG BpmI 569 CTCCAG CTGGAG(16/14) BsaAI 1625 TACGTG [CT]AC^GT[GA] BsgI 677 GTGCAG GTGCAG(16/14) BsiEI 959 CGACCG CG[GA][CT]^CG BsmI 252 GAATGC GAATGC(1/-1) BsoBI 485 CTCGGG C^[CT]CG[GA]G BspLU11I 459 ACATGT A^CATGT BstAPI 1498 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 562 CCTGAGG CC^T[ACGT]AGG Cfr13I 955 GGTCC G^G[ACGT]CC EaeI 1372 TGGCCA [CT]^GGCC[GA] Eco81I 562 CCTGAGG CC^T[ACGT]AGG EcoRI 173 GAATTC G^AATTC HincII 1602 GTCAAC GT[CT]^[GA]AC HindII 1602 GTCAAC GT[CT]^[GA]AC MluNI 1372 TGGCCA TGG^CCA MscI 1372 TGGCCA TGG^CCA NcoI 312 CCATGG C^CATGG Psp1406I 810 AACGTT AA^CGTT SapI 604 GCTCTTC GCTCTTC(1/4) Sau96I 955 GGTCC G^G[ACGT]CC SfcI 519 CTATAG C^T[GA][CT]AG SphI 59 GCATGC GCATG^C StuI 1244 AGGCCT AGG^CCT XbaI 841 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 719 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 895 GAAATTCTTC AspHI 1082 GTGCTC G[AT]GC[AT]^C 1628 GTGCTC BbsI 352 GAAGAC GAAGAC(2/6) 1558 GTCTTC BbvI 631 GCAGC GCAGC(8/12) 685 GCAGC BclI 591 TGATCA T^GATCA 1218 TGATCA BpuAI 352 GAAGAC GAAGAC(2/6) 1558 GTCTTC BsaI 1224 GAGACC GGTCTC(1/5) 1801 GGTCTC BsiHKAI 1082 GTGCTC G[AT]GC[AT]^C 1628 GTGCTC DraI 1018 TTTAAA TTT^AAA 1143 TTTAAA DrdI 231 GACCAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 933 GACTCCTTTGTC EarI 605 CTCTTC CTCTTC(1/4) 1439 GAAGAG FbaI 591 TGATCA T^GATCA 1218 TGATCA Fnu4HI 631 GCAGC GC^[ACGT]GC 685 GCAGC ItaI 631 GCAGC GC^[ACGT]GC 685 GCAGC Ksp632I 605 CTCTTC CTCTTC(1/4) 1439 GAAGAG MaeII 811 ACGT A^CGT 1626 ACGT NdeI 21 CATATG CA^TATG 1278 CATATG PflMI 190 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1029 CCAGATTTTGG SfaNI 1392 GATGC GCATC(5/9) 26 GCATC SspI 1149 AATATT AAT^ATT 1687 AATATT TaqI 207 TCGA T^CGA 1641 TCGA TseI 631 GCAGC G^C[AT]GC 685 GCAGC Tsp45I 13 GTCAC ^GT[GC]AC 75 GTCAC TthHB8I 207 TCGA T^CGA 1641 TCGA Van91I 190 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1029 CCAGATTTTGG XmnI 719 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 895 GAAATTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DsaI EcoRII EcoT14I EcoT22I FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI Sau3AI ScrFI StyI TfiI Tru9I Tsp509I TspRI XhoII