(Linear) (Six-base) MAPSORT of: KIEE4535.seq Check: 6928 from: 1 to: 1806 KIEE4535.orf With 182 enzymes: SgfI * March 22, 2012 18:18 .. AccI GT'mk_AC Cuts at: 0 201 1806 Size: 201 1605 AclI AA'CG_TT Cuts at: 0 811 1806 Size: 811 995 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 592 674 1077 1806 Size: 104 488 82 403 729 Fragments arranged by size: 729 488 403 104 82 AflIII A'CryG_T Cuts at: 0 459 1806 Size: 459 1347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 291 1806 Size: 291 1515 AlwNI CAG_nnn'CTG Cuts at: 0 228 1806 Size: 228 1578 ApoI r'AATT_y Cuts at: 0 173 270 896 1129 1411 1466 1500 1806 Size: 173 97 626 233 282 55 34 306 Fragments arranged by size: 626 306 282 233 173 97 55 34 AvaI C'yCGr_G Cuts at: 0 485 1806 Size: 485 1321 AvrII C'CTAG_G Cuts at: 0 534 1806 Size: 534 1272 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1095 1128 1806 Size: 1095 33 678 Fragments arranged by size: 1095 678 33 BamHI G'GATC_C Cuts at: 0 757 1806 Size: 757 1049 BanI G'GyrC_C Cuts at: 0 529 1806 Size: 529 1277 BbsI GAAGACnn'nnnn_ Cuts at: 0 359 1551 1806 Size: 359 1192 255 Fragments arranged by size: 1192 359 255 BclI T'GATC_A Cuts at: 0 591 1218 1806 Size: 591 627 588 Fragments arranged by size: 627 591 588 BfrBI ATG'CAT Cuts at: 0 3 26 1395 1806 Size: 3 23 1369 411 Fragments arranged by size: 1369 411 23 3 Bme1580I G_kGCm'C Cuts at: 0 534 1806 Size: 534 1272 BmrI ACTGGGnnnn_n' Cuts at: 0 1218 1806 Size: 1218 588 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 554 1806 Size: 554 1252 BsaI GGTCTCn'nnnn_ Cuts at: 0 1218 1806 Size: 1218 588 BsaAI yAC'GTr Cuts at: 0 1627 1806 Size: 1627 179 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1342 1372 1806 Size: 1342 30 434 Fragments arranged by size: 1342 434 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 698 1806 Size: 698 1108 BsiEI CG_ry'CG Cuts at: 0 962 1806 Size: 962 844 BsiHKAI G_wGCw'C Cuts at: 0 1086 1632 1806 Size: 1086 546 174 Fragments arranged by size: 1086 546 174 BsmI GAATG_Cn' Cuts at: 0 258 1806 Size: 258 1548 Bsp1286I G_dGCh'C Cuts at: 0 534 1086 1632 1806 Size: 534 552 546 174 Fragments arranged by size: 552 546 534 174 BstAPI GCAn_nnn'nTGC Cuts at: 0 1504 1806 Size: 1504 302 BstYI r'GATC_y Cuts at: 0 757 885 1477 1806 Size: 757 128 592 329 Fragments arranged by size: 757 592 329 128 Bsu36I CC'TnA_GG Cuts at: 0 563 1806 Size: 563 1243 BtgI C'CryG_G Cuts at: 0 312 962 1369 1806 Size: 312 650 407 437 Fragments arranged by size: 650 437 407 312 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1711 1806 Size: 1711 95 DraI TTT'AAA Cuts at: 0 1020 1145 1806 Size: 1020 125 661 Fragments arranged by size: 1020 661 125 DrdI GACnn_nn'nnGTC Cuts at: 0 237 939 1806 Size: 237 702 867 Fragments arranged by size: 867 702 237 EaeI y'GGCC_r Cuts at: 0 1372 1806 Size: 1372 434 EarI CTCTTCn'nnn_ Cuts at: 0 611 1434 1806 Size: 611 823 372 Fragments arranged by size: 823 611 372 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 554 592 674 1077 1806 Size: 104 450 38 82 403 729 Fragments arranged by size: 729 450 403 104 82 38 EcoRI G'AATT_C Cuts at: 0 173 1806 Size: 173 1633 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 916 1806 Size: 916 890 HincII GTy'rAC Cuts at: 0 1604 1806 Size: 1604 202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 982 1806 Size: 982 824 MscI TGG'CCA Cuts at: 0 1374 1806 Size: 1374 432 MslI CAynn'nnrTG Cuts at: 0 464 985 1400 1806 Size: 464 521 415 406 Fragments arranged by size: 521 464 415 406 NcoI C'CATG_G Cuts at: 0 312 1806 Size: 312 1494 NdeI CA'TA_TG Cuts at: 0 22 1279 1806 Size: 22 1257 527 Fragments arranged by size: 1257 527 22 NsiI A_TGCA'T Cuts at: 0 5 28 1397 1806 Size: 5 23 1369 409 Fragments arranged by size: 1369 409 23 5 NspI r_CATG'y Cuts at: 0 63 463 990 1806 Size: 63 400 527 816 Fragments arranged by size: 816 527 400 63 PciI A'CATG_T Cuts at: 0 459 1806 Size: 459 1347 PflMI CCAn_nnn'nTGG Cuts at: 0 196 1035 1806 Size: 196 839 771 Fragments arranged by size: 839 771 196 PfoI T'CCnGG_A Cuts at: 0 714 1158 1806 Size: 714 444 648 Fragments arranged by size: 714 648 444 PsiI TTA'TAA Cuts at: 0 1271 1806 Size: 1271 535 SapI GCTCTTCn'nnn_ Cuts at: 0 611 1806 Size: 611 1195 SfcI C'TryA_G Cuts at: 0 519 1806 Size: 519 1287 SphI G_CATG'C Cuts at: 0 63 1806 Size: 63 1743 SspI AAT'ATT Cuts at: 0 1151 1689 1806 Size: 1151 538 117 Fragments arranged by size: 1151 538 117 StuI AGG'CCT Cuts at: 0 1246 1806 Size: 1246 560 StyI C'CwwG_G Cuts at: 0 312 534 772 1247 1289 1806 Size: 312 222 238 475 42 517 Fragments arranged by size: 517 475 312 238 222 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 517 1030 1240 1806 Size: 517 513 210 566 Fragments arranged by size: 566 517 513 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 411 443 1342 1374 1806 Size: 411 32 899 32 432 Fragments arranged by size: 899 432 411 32 32 XbaI T'CTAG_A Cuts at: 0 841 1806 Size: 841 965 XmnI GAAnn'nnTTC Cuts at: 0 723 899 1806 Size: 723 176 907 Fragments arranged by size: 907 723 176 Enzymes that do cut: AccI AclI AcuI AflIII AlfI AlwNI ApoI AvaI AvrII BaeI BamHI BanI BbsI BclI BfrBI Bme1580I BmrI BpmI BsaI BsaAI BsaXI BsgI BsiEI BsiHKAI BsmI Bsp1286I BstAPI BstYI Bsu36I BtgI BtgZI DraI DrdI EaeI EarI Eco57MI EcoRI FalI HincII MmeI MscI MslI NcoI NdeI NsiI NspI PciI PflMI PfoI PsiI SapI SfcI SphI SspI StuI StyI TaqII TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI TatI Tth111I XcmI XhoI XmaI ZraI