################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccI AclI AflII AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BbeI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspHI BsrGI BssHII Bst1107I BstBI BstZ17I CelII CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI HindIII HpaI KasI KpnI MamI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfoI SfuI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 14 TCCGGA T^CCGGA AcsI 2419 GAATTC [GA]^AATT[CT] AfaI 906 GTAC GT^AC AflIII 1835 ACATGT A^C[GA][CT]GT AgeI 1513 ACCGGT A^CCGGT AocI 492 CCTGAGG CC^T[ACGT]AGG ApoI 2419 GAATTC [GA]^AATT[CT] BamHI 1500 GGATCC G^GATCC BbvCI 412 CCTCAGC CCTCAGC(-5/-2) BglII 1304 AGATCT A^GATCT BseAI 14 TCCGGA T^CCGGA BsiEI 1276 CGACCG CG[GA][CT]^CG BsmI 1468 GCATTC GAATGC(1/-1) BspDI 1546 ATCGAT AT^CGAT BspEI 14 TCCGGA T^CCGGA BspLU11I 1835 ACATGT A^CATGT BstEII 1744 GGTTACC G^GT[ACGT]ACC BstPI 1744 GGTTACC G^GT[ACGT]ACC Bsu36I 492 CCTGAGG CC^T[ACGT]AGG ClaI 1546 ATCGAT AT^CGAT DrdI 490 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 492 CCTGAGG CC^T[ACGT]AGG EcoO65I 1744 GGTTACC G^GT[ACGT]ACC EcoRI 2419 GAATTC G^AATTC EcoT22I 1466 ATGCAT ATGCA^T KspI 570 CCGCGG CCGC^GG MroI 14 TCCGGA T^CCGGA NaeI 258 GCCGGC GCC^GGC NgoMIV 258 GCCGGC G^CCGGC NsiI 1466 ATGCAT ATGCA^T PinAI 1513 ACCGGT A^CCGGT RsaI 906 GTAC GT^AC SacII 570 CCGCGG CCGC^GG SgrAI 1512 CACCGGTG C[GA]^CCGG[CT]G SmlI 1578 CTTGAG C^T[CT][GA]AG Tsp509I 2420 AATT ^AATT XcmI 1668 CCACGTGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 453 GACGCC G[GA]^CG[CT]C 1624 GGCGTC Alw44I 1630 GTGCAC G^TGCAC 1672 GTGCAC ApaLI 1630 GTGCAC G^TGCAC 1672 GTGCAC Asp700I 186 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2427 GAACAAGTTC AviII 284 TGCGCA TGC^GCA 2267 TGCGCA BbrPI 642 CACGTG CAC^GTG 1669 CACGTG BsaAI 642 CACGTG [CT]AC^GT[GA] 1669 CACGTG BsaHI 453 GACGCC G[GA]^CG[CT]C 1624 GGCGTC BsaI 113 GAGACC GGTCTC(1/5) 980 GAGACC BsaWI 14 TCCGGA [AT]^CCGG[AT] 1513 ACCGGT BsrBI 1163 CCGCTC CCGCTC(-3/-3) 823 GAGCGG BsrDI 582 CATTGC GCAATG(2/0) 954 CATTGC BssSI 100 CTCGTG CACGAG(-5/-1) 1750 CTCGTG DraIII 619 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 2410 CACTGGGTG FspI 284 TGCGCA TGC^GCA 2267 TGCGCA Hin1I 453 GACGCC G[GA]^CG[CT]C 1624 GGCGTC HincII 499 GTCAAC GT[CT]^[GA]AC 1156 GTCAAC HindII 499 GTCAAC GT[CT]^[GA]AC 1156 GTCAAC NspI 1067 ACATGC [GA]CATG^[CT] 1835 ACATGT PmaCI 642 CACGTG CAC^GTG 1669 CACGTG PmlI 642 CACGTG CAC^GTG 1669 CACGTG SbfI 201 CCTGCAGG CCTGCA^GG 993 CCTGCAGG SmaI 272 CCCGGG CCC^GGG 472 CCCGGG Sse8387I 201 CCTGCAGG CCTGCA^GG 993 CCTGCAGG StuI 962 AGGCCT AGG^CCT 2274 AGGCCT Tsp45I 625 GTGAC ^GT[GC]AC 1510 GTCAC XmaCI 272 CCCGGG C^CCGGG 472 CCCGGG XmaI 272 CCCGGG C^CCGGG 472 CCCGGG XmnI 186 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2427 GAACAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI StyI TaqI TseI TspRI TthHB8I XhoII