(Circular) (Six-base) MAPSORT of: pFN21AE1482.seq Check: 2778 from: 1 to: 7195 pFN21AE1482 7195 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2084 2507 2872 3975 2084 Size: 423 365 1103 5304 Fragments arranged by size: 5304 1103 423 365 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5005 278 Size: 53 83 186 936 3469 2468 Fragments arranged by size: 3469 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4538 1937 Size: 2601 4594 Acc65I G'GTAC_C Cuts at: 4516 4516 Size: 7195 AclI AA'CG_TT Cuts at: 1563 5322 5695 1563 Size: 3759 373 3063 Fragments arranged by size: 3759 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2581 3310 3382 5258 6642 6910 1632 Size: 949 729 72 1876 1384 268 1917 Fragments arranged by size: 1917 1876 1384 949 729 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6998 AflIII A'CryG_T Cuts at: 1203 3840 6094 7071 1203 Size: 2637 2254 977 1327 Fragments arranged by size: 2637 2254 1327 977 AgeI A'CCGG_T Cuts at: 3518 6026 3518 Size: 2508 4687 AhdI GACnn_n'nnGTC Cuts at: 5924 5924 Size: 7195 AleI CACnn'nnGTG Cuts at: 1166 2078 3911 1166 Size: 912 1833 4450 Fragments arranged by size: 4450 1833 912 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3890 3977 1184 Size: 2706 87 4402 Fragments arranged by size: 4402 2706 87 AlwNI CAG_nnn'CTG Cuts at: 2066 2326 2356 2497 2617 2782 2803 2869 4938 Size: 260 30 141 120 165 21 66 2069 Cuts at: 4938 6510 2066 Size: 1572 2751 Fragments arranged by size: 2751 2069 1572 260 165 141 120 66 30 21 ApaI G_GGCC'C Cuts at: 2365 2773 4222 2365 Size: 408 1449 5338 Fragments arranged by size: 5338 1449 408 ApaLI G'TGCA_C Cuts at: 3635 3677 5252 6408 3635 Size: 42 1575 1156 4422 Fragments arranged by size: 4422 1575 1156 42 ApoI r'AATT_y Cuts at: 1493 4424 4504 4730 4882 6991 1493 Size: 2931 80 226 152 2109 1697 Fragments arranged by size: 2931 2109 1697 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 5749 160 Size: 872 4717 1606 Fragments arranged by size: 4717 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7195 AvaI C'yCGr_G Cuts at: 1870 1943 2277 2477 2765 2816 3424 4520 1870 Size: 73 334 200 288 51 608 1096 4545 Fragments arranged by size: 4545 1096 608 334 288 200 73 51 BamHI G'GATC_C Cuts at: 3505 4525 4892 3505 Size: 1020 367 5808 Fragments arranged by size: 5808 1020 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 2519 3693 3934 4516 Size: 325 217 627 479 253 1174 241 582 Cuts at: 4516 5971 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1174 627 582 479 325 253 241 217 BanII G_rGCy'C Cuts at: 1391 2012 2365 2532 2773 3726 4076 4125 4222 Size: 621 353 167 241 953 350 49 97 Cuts at: 4222 4514 1391 Size: 292 4072 Fragments arranged by size: 4072 953 621 353 350 292 241 167 97 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2198 2725 3517 3658 928 Size: 971 299 527 792 141 4465 Fragments arranged by size: 4465 971 792 527 299 141 BbvCI CC'TCA_GC Cuts at: 2418 2418 Size: 7195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5386 5420 1903 Size: 34 3449 34 3678 Fragments arranged by size: 3678 3449 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5087 6297 5087 Size: 1210 5985 BclI T'GATC_A Cuts at: 1548 1548 Size: 7195 BfrBI ATG'CAT Cuts at: 3473 3473 Size: 7195 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2360 2878 3446 Size: 107 122 71 631 605 688 518 568 Cuts at: 3446 3809 5806 136 Size: 363 1997 1525 Fragments arranged by size: 1997 1525 688 631 605 568 518 363 122 107 71 BglII A'GATC_T Cuts at: 3309 7190 3309 Size: 3881 3314 BlpI GC'TnA_GC Cuts at: 4612 4612 Size: 7195 Bme1580I G_kGCm'C Cuts at: 1790 2365 2773 3639 3681 3939 4222 5256 6412 Size: 575 408 866 42 258 283 1034 1156 Cuts at: 6412 1790 Size: 2573 Fragments arranged by size: 2573 1156 1034 866 575 408 283 258 42 BmgBI CAC'GTC Cuts at: 1318 2731 1318 Size: 1413 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2683 4426 4926 Size: 416 53 83 399 24 1266 1743 500 Cuts at: 4926 5884 442 Size: 958 1753 Fragments arranged by size: 1753 1743 1266 958 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7195 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4402 4402 Size: 7195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2453 2584 2627 2639 3451 3484 3728 Size: 968 246 131 43 12 812 33 244 Cuts at: 3728 4090 5855 1239 Size: 362 1765 2579 Fragments arranged by size: 2579 1765 968 812 362 246 244 131 43 33 12 Bpu10I CC'TnA_GC Cuts at: 2418 2837 4223 2418 Size: 419 1386 5390 Fragments arranged by size: 5390 1386 419 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3604 5320 6185 6483 3604 Size: 1716 865 298 4316 Fragments arranged by size: 4316 1716 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 2979 5858 882 Size: 617 613 867 2879 2219 Fragments arranged by size: 2879 2219 867 617 613 BsaAI yAC'GTr Cuts at: 493 1204 2649 3676 4986 493 Size: 711 1445 1027 1310 2702 Fragments arranged by size: 2702 1445 1310 1027 711 BsaBI GATnn'nnATC Cuts at: 7189 7189 Size: 7195 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2459 3630 5002 5384 Size: 53 83 186 936 926 1171 1372 382 Cuts at: 5384 6019 275 Size: 635 1451 Fragments arranged by size: 1451 1372 1171 936 926 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 3518 5628 6026 6300 6447 6858 2019 Size: 1499 2110 398 274 147 411 2356 Fragments arranged by size: 2356 2110 1499 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4184 4214 1178 Size: 30 165 30 2781 30 4159 Fragments arranged by size: 4159 2781 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2195 2228 2315 2768 3044 4012 4127 Size: 856 153 33 87 453 276 968 115 Cuts at: 4127 1186 Size: 4254 Fragments arranged by size: 4254 968 856 453 276 153 115 87 33 BseYI C'CCAG_C Cuts at: 2089 2098 2228 2364 3483 3736 3815 3863 4239 Size: 9 130 136 1119 253 79 48 376 Cuts at: 4239 6398 6894 2089 Size: 2159 496 2390 Fragments arranged by size: 2390 2159 1119 496 376 253 136 130 79 48 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2906 2950 3947 3956 4382 4397 Size: 573 618 552 44 997 9 426 15 Cuts at: 4397 1163 Size: 3961 Fragments arranged by size: 3961 997 618 573 552 426 44 15 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3284 4645 5406 6434 1540 Size: 275 186 1283 1361 761 1028 2301 Fragments arranged by size: 2301 1361 1283 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2625 2942 2978 3639 3681 3726 4076 4125 4143 Size: 317 36 661 42 45 350 49 18 Cuts at: 4143 5256 5341 6412 2625 Size: 1113 85 1071 3408 Fragments arranged by size: 3408 1113 1071 661 350 317 85 49 45 42 36 18 BsmI GAATG_Cn' Cuts at: 3473 4706 4799 3473 Size: 1233 93 5869 Fragments arranged by size: 5869 1233 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2143 2297 3704 4192 2143 Size: 154 1407 488 5146 Fragments arranged by size: 5146 1407 488 154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2365 2532 2625 2773 2942 2978 Size: 399 222 353 167 93 148 169 36 Cuts at: 2978 3639 3681 3726 3939 4076 4125 4143 4222 Size: 661 42 45 213 137 49 18 79 Cuts at: 4222 4514 5256 5341 6412 1391 Size: 292 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 399 353 292 222 213 169 167 148 137 93 85 79 49 45 42 36 18 BspEI T'CCGG_A Cuts at: 2019 6858 2019 Size: 4839 2356 BspHI T'CATG_A Cuts at: 5084 6814 5084 Size: 1730 5465 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2084 2292 2507 2872 2878 2997 3348 3975 Size: 1240 208 215 365 6 119 351 627 Cuts at: 3975 4053 4215 4550 844 Size: 78 162 335 3489 Fragments arranged by size: 3489 1240 627 365 351 335 215 208 162 119 78 6 BsrBI CCG'CTC Cuts at: 1394 1823 2830 3170 5082 1394 Size: 429 1007 340 1912 3507 Fragments arranged by size: 3507 1912 1007 429 340 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 2958 5690 5864 65 Size: 1183 1338 372 2732 174 1396 Fragments arranged by size: 2732 1396 1338 1183 372 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2263 3283 3374 3518 3696 5839 6026 Size: 110 453 1020 91 144 178 2143 187 Cuts at: 6026 1700 Size: 2869 Fragments arranged by size: 2869 2143 1020 453 187 178 144 110 91 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7195 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7195 BssSI C'ACGA_G Cuts at: 2105 3755 5255 6267 2105 Size: 1650 1500 1012 3033 Fragments arranged by size: 3033 1650 1500 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 2377 2782 2869 3063 3493 3700 3970 Size: 1562 21 405 87 194 430 207 270 Cuts at: 3970 4405 794 Size: 435 3584 Fragments arranged by size: 3584 1562 435 430 405 270 207 194 87 21 BstBI TT'CG_AA Cuts at: 5999 5999 Size: 7195 BstEII G'GTnAC_C Cuts at: 1184 3749 1184 Size: 2565 4630 BstXI CCAn_nnnn'nTGG Cuts at: 2441 2649 3913 2441 Size: 208 1264 5723 Fragments arranged by size: 5723 1264 208 BstYI r'GATC_y Cuts at: 1972 2697 3309 3505 4525 4892 5277 5294 6746 Size: 725 612 196 1020 367 385 17 1452 Cuts at: 6746 6832 6844 7190 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1020 725 612 385 367 346 196 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2498 2498 Size: 7195 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 2575 3045 3229 3261 3444 Size: 552 939 519 52 470 184 32 183 Cuts at: 3444 3930 4263 4356 513 Size: 486 333 93 3352 Fragments arranged by size: 3352 939 552 519 486 470 333 184 183 93 52 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6456 Fragments arranged by size: 6456 683 56 BtsI GCAGTG_nn' Cuts at: 2722 4711 5505 5525 2722 Size: 1989 794 20 4392 Fragments arranged by size: 4392 1989 794 20 ClaI AT'CG_AT Cuts at: 1554 3552 1554 Size: 1998 5197 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7160 DraI TTT'AAA Cuts at: 4499 4499 Size: 7195 DraIII CAC_nnn'GTG Cuts at: 2629 4420 2629 Size: 1791 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2501 6202 809 Size: 507 1185 3701 1802 Fragments arranged by size: 3701 1802 1185 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3108 3258 3438 Size: 54 1425 50 275 18 1278 150 180 Cuts at: 3438 3447 3685 3927 4642 5531 7160 8 Size: 9 238 242 715 889 1629 43 Fragments arranged by size: 1629 1425 1278 889 715 275 242 238 180 150 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4642 1537 Size: 275 2830 4090 Fragments arranged by size: 4090 2830 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3254 3749 3854 4104 5125 1353 Size: 1901 495 105 250 1021 3423 Fragments arranged by size: 3423 1901 1021 495 250 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5782 6156 6302 387 Size: 1064 138 4193 374 146 1280 Fragments arranged by size: 4193 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3724 4074 4123 3724 Size: 350 49 6796 Fragments arranged by size: 6796 350 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2453 2581 2584 2627 2639 3310 Size: 393 575 246 128 3 43 12 671 Cuts at: 3310 3382 3451 3484 3728 4090 5258 5855 6642 Size: 72 69 33 244 362 1168 597 787 Cuts at: 6642 6910 1239 Size: 268 1524 Fragments arranged by size: 1524 1168 787 671 597 575 393 362 268 246 244 128 72 69 43 33 12 3 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2318 2452 2468 2840 3002 2210 Size: 108 134 16 372 162 6403 Fragments arranged by size: 6403 372 162 134 108 16 EcoO109I rG'GnC_Cy Cuts at: 2281 2353 2445 2473 2494 2539 2568 2769 2770 Size: 72 92 28 21 45 29 201 1 Cuts at: 2770 2812 3007 3533 4096 4219 2281 Size: 42 195 526 563 123 5257 Fragments arranged by size: 5257 563 526 201 195 123 92 72 45 42 29 28 21 1 EcoRI G'AATT_C Cuts at: 4424 4504 4882 4424 Size: 80 378 6737 Fragments arranged by size: 6737 378 80 FspI TGC'GCA Cuts at: 2291 4274 5701 2291 Size: 1983 1427 3785 Fragments arranged by size: 3785 1983 1427 FspAI rTGC'GCAy Cuts at: 4274 4274 Size: 7195 HaeII r_GCGC'y Cuts at: 1678 2142 2176 2314 2557 6342 1678 Size: 464 34 138 243 3785 2531 Fragments arranged by size: 3785 2531 464 243 138 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2432 2464 2682 2714 5836 Size: 32 135 32 860 32 218 32 3122 Cuts at: 5836 5868 5910 5942 1373 Size: 32 42 32 2626 Fragments arranged by size: 3122 2626 860 218 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3163 4539 4785 669 Size: 988 281 568 657 1376 246 3079 Fragments arranged by size: 3079 1376 988 657 568 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7195 HpaI GTT'AAC Cuts at: 4785 4785 Size: 7195 KpnI G_GTAC'C Cuts at: 4520 4520 Size: 7195 MfeI C'AATT_G Cuts at: 4794 4794 Size: 7195 MluI A'CGCG_T Cuts at: 7071 7071 Size: 7195 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4215 6309 6493 1305 Size: 2910 2094 184 2007 Fragments arranged by size: 2910 2094 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3260 3449 3687 3929 10 Size: 54 1425 343 1428 189 238 242 3276 Fragments arranged by size: 3276 1428 1425 343 242 238 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2439 2651 3839 3911 4268 5153 Size: 648 912 361 212 1188 72 357 885 Cuts at: 5153 5512 5671 518 Size: 359 159 2042 Fragments arranged by size: 2042 1188 912 885 648 361 359 357 212 159 72 MspA1I CmG'CkG Cuts at: 1606 2063 2368 2377 2422 2577 2617 2782 2866 Size: 457 305 9 45 155 40 165 84 Cuts at: 2866 4007 4243 4611 5288 6436 6681 1606 Size: 1141 236 368 677 1148 245 2120 Fragments arranged by size: 2120 1148 1141 677 457 368 305 245 236 165 155 84 45 40 9 NaeI GCC'GGC Cuts at: 2265 2265 Size: 7195 NcoI C'CATG_G Cuts at: 513 1065 2004 3261 3444 3930 4263 513 Size: 552 939 1257 183 486 333 3445 Fragments arranged by size: 3445 1257 939 552 486 333 183 NdeI CA'TA_TG Cuts at: 387 387 Size: 7195 NgoMIV G'CCGG_C Cuts at: 2263 2263 Size: 7195 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7195 NotI GC'GGCC_GC Cuts at: 4642 4642 Size: 7195 NsiI A_TGCA'T Cuts at: 3475 3475 Size: 7195 NspI r_CATG'y Cuts at: 1132 1229 3076 3844 4553 6098 1132 Size: 97 1847 768 709 1545 2229 Fragments arranged by size: 2229 1847 1545 768 709 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7195 PciI A'CATG_T Cuts at: 3840 6094 3840 Size: 2254 4941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7195 PfoI T'CCnGG_A Cuts at: 2295 2295 Size: 7195 PmeI GTTT'AAAC Cuts at: 4499 4499 Size: 7195 PmlI CAC'GTG Cuts at: 2649 3676 2649 Size: 1027 6168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5264 5296 6777 6809 5264 Size: 32 1481 32 5650 Fragments arranged by size: 5650 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2494 2812 3533 2281 Size: 213 318 721 5943 Fragments arranged by size: 5943 721 318 213 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7195 PsiI TTA'TAA Cuts at: 4765 4765 Size: 7195 PspOMI G'GGCC_C Cuts at: 2361 2769 4218 2361 Size: 408 1449 5338 Fragments arranged by size: 5338 1449 408 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4424 4456 4424 Size: 32 7163 PstI C_TGCA'G Cuts at: 830 2211 2382 2748 2778 2808 2865 3003 4547 Size: 1381 171 366 30 30 57 138 1544 Cuts at: 4547 830 Size: 3478 Fragments arranged by size: 3478 1544 1381 366 171 138 57 30 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7195 PvuII CAG'CTG Cuts at: 2368 2377 2422 2782 2866 4243 2368 Size: 9 45 360 84 1377 5320 Fragments arranged by size: 5320 1377 360 84 45 9 SacI G_AGCT'C Cuts at: 3726 4076 4125 3726 Size: 350 49 6796 Fragments arranged by size: 6796 350 49 SacII CC_GC'GG Cuts at: 2578 2578 Size: 7195 SalI G'TCGA_C Cuts at: 1936 4537 1936 Size: 2601 4594 SanDI GG'GwC_CC Cuts at: 3533 3533 Size: 7195 SbfI CC_TGCA'GG Cuts at: 2211 3003 4547 2211 Size: 792 1544 4859 Fragments arranged by size: 4859 1544 792 ScaI AGT'ACT Cuts at: 5443 7155 5443 Size: 1712 5483 SfcI C'TryA_G Cuts at: 826 1045 2207 2378 2744 2774 2804 2861 2999 Size: 219 1162 171 366 30 30 57 138 Cuts at: 2999 4543 5678 6359 6550 826 Size: 1544 1135 681 191 1471 Fragments arranged by size: 1544 1471 1162 1135 681 366 219 191 171 138 57 30 30 SfiI GGCCn_nnn'nGGCC Cuts at: 2360 3446 3809 2360 Size: 1086 363 5746 Fragments arranged by size: 5746 1086 363 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7195 SgrAI Cr'CCGG_yG Cuts at: 1700 3518 1700 Size: 1818 5377 SmaI CCC'GGG Cuts at: 1872 2279 2479 4522 1872 Size: 407 200 2043 4545 Fragments arranged by size: 4545 2043 407 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 3583 5299 6200 6462 820 Size: 197 926 1640 1716 901 262 1553 Fragments arranged by size: 1716 1640 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4986 493 Size: 4493 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7195 SphI G_CATG'C Cuts at: 1132 4553 1132 Size: 3421 3774 SspI AAT'ATT Cuts at: 5 52 5119 5 Size: 47 5067 2081 Fragments arranged by size: 5067 2081 47 StuI AGG'CCT Cuts at: 1517 2969 4281 1517 Size: 1452 1312 4431 Fragments arranged by size: 4431 1452 1312 StyI C'CwwG_G Cuts at: 513 1065 2004 3261 3444 3924 3930 4263 4634 Size: 552 939 1257 183 480 6 333 371 Cuts at: 4634 513 Size: 3074 Fragments arranged by size: 3074 1257 939 552 480 371 333 183 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4026 4408 5222 5239 5392 5577 6022 Size: 1255 2446 382 814 17 153 185 445 Cuts at: 6022 325 Size: 1498 Fragments arranged by size: 2446 1498 1255 814 445 382 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5441 7153 96 Size: 274 80 33 51 1443 3464 1712 138 Fragments arranged by size: 3464 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7155 7187 7155 Size: 32 7163 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7195 XbaI T'CTAG_A Cuts at: 4531 4531 Size: 7195 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3680 3680 Size: 7195 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7195 XmaI C'CCGG_G Cuts at: 1870 2277 2477 4520 1870 Size: 407 200 2043 4545 Fragments arranged by size: 4545 2043 407 200 XmnI GAAnn'nnTTC Cuts at: 2195 4436 5324 2195 Size: 2241 888 4066 Fragments arranged by size: 4066 2241 888 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5003 276 Size: 53 83 186 936 3469 2468 Fragments arranged by size: 3469 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FalI FseI KasI NarI NruI PacI PspXI RsrII SapI SexAI SfoI SrfI SwaI