(Linear) (Six-base) MAPSORT of: KIEE1482.seq Check: 4343 from: 1 to: 2490 KIEE1482.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 79 502 867 1970 2490 Size: 79 423 365 1103 520 Fragments arranged by size: 1103 520 423 365 79 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 576 1305 1377 2490 Size: 576 729 72 1113 Fragments arranged by size: 1113 729 576 72 AflIII A'CryG_T Cuts at: 0 1835 2490 Size: 1835 655 AgeI A'CCGG_T Cuts at: 0 1513 2490 Size: 1513 977 AleI CACnn'nnGTG Cuts at: 0 73 1906 2490 Size: 73 1833 584 Fragments arranged by size: 1833 584 73 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1885 1972 2490 Size: 1885 87 518 Fragments arranged by size: 1885 518 87 AlwNI CAG_nnn'CTG Cuts at: 0 61 321 351 492 612 777 798 864 Size: 61 260 30 141 120 165 21 66 Cuts at: 864 2490 Size: 1626 Fragments arranged by size: 1626 260 165 141 120 66 61 30 21 ApaI G_GGCC'C Cuts at: 0 360 768 2217 2490 Size: 360 408 1449 273 Fragments arranged by size: 1449 408 360 273 ApaLI G'TGCA_C Cuts at: 0 1630 1672 2490 Size: 1630 42 818 Fragments arranged by size: 1630 818 42 ApoI r'AATT_y Cuts at: 0 2419 2490 Size: 2419 71 AvaI C'yCGr_G Cuts at: 0 272 472 760 811 1419 2490 Size: 272 200 288 51 608 1071 Fragments arranged by size: 1071 608 288 272 200 51 BamHI G'GATC_C Cuts at: 0 1500 2490 Size: 1500 990 BanI G'GyrC_C Cuts at: 0 261 514 1688 1929 2490 Size: 261 253 1174 241 561 Fragments arranged by size: 1174 561 261 253 241 BanII G_rGCy'C Cuts at: 0 7 360 527 768 1721 2071 2120 2217 Size: 7 353 167 241 953 350 49 97 Cuts at: 2217 2490 Size: 273 Fragments arranged by size: 953 353 350 273 241 167 97 49 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 193 720 1512 1653 2490 Size: 193 527 792 141 837 Fragments arranged by size: 837 792 527 193 141 BbvCI CC'TCA_GC Cuts at: 0 413 2490 Size: 413 2077 BfrBI ATG'CAT Cuts at: 0 1468 2490 Size: 1468 1022 BglI GCCn_nnn'nGGC Cuts at: 0 355 873 1441 1804 2490 Size: 355 518 568 363 686 Fragments arranged by size: 686 568 518 363 355 BglII A'GATC_T Cuts at: 0 1304 2490 Size: 1304 1186 Bme1580I G_kGCm'C Cuts at: 0 360 768 1634 1676 1934 2217 2490 Size: 360 408 866 42 258 283 273 Fragments arranged by size: 866 408 360 283 273 258 42 BmgBI CAC'GTC Cuts at: 0 726 2490 Size: 726 1764 BmrI ACTGGGnnnn_n' Cuts at: 0 678 2421 2490 Size: 678 1743 69 Fragments arranged by size: 1743 678 69 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2397 2490 Size: 2397 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 448 579 622 634 1446 1479 1723 Size: 202 246 131 43 12 812 33 244 Cuts at: 1723 2085 2490 Size: 362 405 Fragments arranged by size: 812 405 362 246 244 202 131 43 33 12 Bpu10I CC'TnA_GC Cuts at: 0 413 832 2218 2490 Size: 413 419 1386 272 Fragments arranged by size: 1386 419 413 272 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1599 2490 Size: 1599 891 BsaI GGTCTCn'nnnn_ Cuts at: 0 107 974 2490 Size: 107 867 1516 Fragments arranged by size: 1516 867 107 BsaAI yAC'GTr Cuts at: 0 644 1671 2490 Size: 644 1027 819 Fragments arranged by size: 1027 819 644 BsaHI Gr'CG_yC Cuts at: 0 454 1625 2490 Size: 454 1171 865 Fragments arranged by size: 1171 865 454 BsaWI w'CCGG_w Cuts at: 0 14 1513 2490 Size: 14 1499 977 Fragments arranged by size: 1499 977 14 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2179 2209 2490 Size: 2179 30 281 Fragments arranged by size: 2179 281 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 190 223 310 763 1039 2007 2122 Size: 37 153 33 87 453 276 968 115 Cuts at: 2122 2490 Size: 368 Fragments arranged by size: 968 453 368 276 153 115 87 37 33 BseYI C'CCAG_C Cuts at: 0 84 93 223 359 1478 1731 1810 1858 Size: 84 9 130 136 1119 253 79 48 Cuts at: 1858 2234 2490 Size: 376 256 Fragments arranged by size: 1119 376 256 253 136 130 84 79 48 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 901 945 1942 1951 2377 2392 2490 Size: 349 552 44 997 9 426 15 98 Fragments arranged by size: 997 552 426 349 98 44 15 9 BsiEI CG_ry'CG Cuts at: 0 1279 2490 Size: 1279 1211 BsiHKAI G_wGCw'C Cuts at: 0 620 937 973 1634 1676 1721 2071 2120 Size: 620 317 36 661 42 45 350 49 Cuts at: 2120 2138 2490 Size: 18 352 Fragments arranged by size: 661 620 352 350 317 49 45 42 36 18 BsmI GAATG_Cn' Cuts at: 0 1468 2490 Size: 1468 1022 BsmBI CGTCTCn'nnnn_ Cuts at: 0 138 292 1699 2187 2490 Size: 138 154 1407 488 303 Fragments arranged by size: 1407 488 303 154 138 Bsp1286I G_dGCh'C Cuts at: 0 7 360 527 620 768 937 973 1634 Size: 7 353 167 93 148 169 36 661 Cuts at: 1634 1676 1721 1934 2071 2120 2138 2217 2490 Size: 42 45 213 137 49 18 79 273 Fragments arranged by size: 661 353 273 213 169 167 148 137 93 79 49 45 42 36 18 7 BspEI T'CCGG_A Cuts at: 0 14 2490 Size: 14 2476 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 79 287 502 867 873 992 1343 1970 Size: 79 208 215 365 6 119 351 627 Cuts at: 1970 2048 2210 2490 Size: 78 162 280 Fragments arranged by size: 627 365 351 280 215 208 162 119 79 78 6 BsrBI CCG'CTC Cuts at: 0 825 1165 2490 Size: 825 340 1325 Fragments arranged by size: 1325 825 340 BsrDI GCAATG_nn' Cuts at: 0 581 953 2490 Size: 581 372 1537 Fragments arranged by size: 1537 581 372 BsrFI r'CCGG_y Cuts at: 0 258 1278 1369 1513 1691 2490 Size: 258 1020 91 144 178 799 Fragments arranged by size: 1020 799 258 178 144 91 BssSI C'ACGA_G Cuts at: 0 100 1750 2490 Size: 100 1650 740 Fragments arranged by size: 1650 740 100 BstAPI GCAn_nnn'nTGC Cuts at: 0 351 372 777 864 1058 1488 1695 1965 Size: 351 21 405 87 194 430 207 270 Cuts at: 1965 2400 2490 Size: 435 90 Fragments arranged by size: 435 430 405 351 270 207 194 90 87 21 BstEII G'GTnAC_C Cuts at: 0 1744 2490 Size: 1744 746 BstXI CCAn_nnnn'nTGG Cuts at: 0 436 644 1908 2490 Size: 436 208 1264 582 Fragments arranged by size: 1264 582 436 208 BstYI r'GATC_y Cuts at: 0 692 1304 1500 2490 Size: 692 612 196 990 Fragments arranged by size: 990 692 612 196 Bsu36I CC'TnA_GG Cuts at: 0 493 2490 Size: 493 1997 BtgI C'CryG_G Cuts at: 0 518 570 1040 1224 1256 1439 1925 2258 Size: 518 52 470 184 32 183 486 333 Cuts at: 2258 2351 2490 Size: 93 139 Fragments arranged by size: 518 486 470 333 184 183 139 93 52 32 BtsI GCAGTG_nn' Cuts at: 0 717 2490 Size: 717 1773 ClaI AT'CG_AT Cuts at: 0 1547 2490 Size: 1547 943 DraIII CAC_nnn'GTG Cuts at: 0 624 2415 2490 Size: 624 1791 75 Fragments arranged by size: 1791 624 75 DrdI GACnn_nn'nnGTC Cuts at: 0 496 2490 Size: 496 1994 EaeI y'GGCC_r Cuts at: 0 1103 1253 1433 1442 1680 1922 2490 Size: 1103 150 180 9 238 242 568 Fragments arranged by size: 1103 568 242 238 180 150 9 EarI CTCTTCn'nnn_ Cuts at: 0 1249 1744 1849 2099 2490 Size: 1249 495 105 250 391 Fragments arranged by size: 1249 495 391 250 105 EcoICRI GAG'CTC Cuts at: 0 1719 2069 2118 2490 Size: 1719 350 49 372 Fragments arranged by size: 1719 372 350 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 448 576 579 622 634 1305 1377 Size: 202 246 128 3 43 12 671 72 Cuts at: 1377 1446 1479 1723 2085 2490 Size: 69 33 244 362 405 Fragments arranged by size: 671 405 362 246 244 202 128 72 69 43 33 12 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 205 313 447 463 835 997 2490 Size: 205 108 134 16 372 162 1493 Fragments arranged by size: 1493 372 205 162 134 108 16 EcoO109I rG'GnC_Cy Cuts at: 0 276 348 440 468 489 534 563 764 Size: 276 72 92 28 21 45 29 201 Cuts at: 764 765 807 1002 1528 2091 2214 2490 Size: 1 42 195 526 563 123 276 Fragments arranged by size: 563 526 276 276 201 195 123 92 72 45 42 29 28 21 1 EcoRI G'AATT_C Cuts at: 0 2419 2490 Size: 2419 71 FspI TGC'GCA Cuts at: 0 286 2269 2490 Size: 286 1983 221 Fragments arranged by size: 1983 286 221 FspAI rTGC'GCAy Cuts at: 0 2269 2490 Size: 2269 221 HaeII r_GCGC'y Cuts at: 0 137 171 309 552 2490 Size: 137 34 138 243 1938 Fragments arranged by size: 1938 243 138 137 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 427 459 677 709 2490 Size: 427 32 218 32 1781 Fragments arranged by size: 1781 427 218 32 32 HincII GTy'rAC Cuts at: 0 501 1158 2490 Size: 501 657 1332 Fragments arranged by size: 1332 657 501 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2210 2490 Size: 2210 280 MscI TGG'CCA Cuts at: 0 1255 1444 1682 1924 2490 Size: 1255 189 238 242 566 Fragments arranged by size: 1255 566 242 238 189 MslI CAynn'nnrTG Cuts at: 0 73 434 646 1834 1906 2263 2490 Size: 73 361 212 1188 72 357 227 Fragments arranged by size: 1188 361 357 227 212 73 72 MspA1I CmG'CkG Cuts at: 0 58 363 372 417 572 612 777 861 Size: 58 305 9 45 155 40 165 84 Cuts at: 861 2002 2238 2490 Size: 1141 236 252 Fragments arranged by size: 1141 305 252 236 165 155 84 58 45 40 9 NaeI GCC'GGC Cuts at: 0 260 2490 Size: 260 2230 NcoI C'CATG_G Cuts at: 0 1256 1439 1925 2258 2490 Size: 1256 183 486 333 232 Fragments arranged by size: 1256 486 333 232 183 NgoMIV G'CCGG_C Cuts at: 0 258 2490 Size: 258 2232 NsiI A_TGCA'T Cuts at: 0 1470 2490 Size: 1470 1020 NspI r_CATG'y Cuts at: 0 1071 1839 2490 Size: 1071 768 651 Fragments arranged by size: 1071 768 651 PciI A'CATG_T Cuts at: 0 1835 2490 Size: 1835 655 PfoI T'CCnGG_A Cuts at: 0 290 2490 Size: 290 2200 PmlI CAC'GTG Cuts at: 0 644 1671 2490 Size: 644 1027 819 Fragments arranged by size: 1027 819 644 PpuMI rG'GwC_Cy Cuts at: 0 276 489 807 1528 2490 Size: 276 213 318 721 962 Fragments arranged by size: 962 721 318 276 213 PspOMI G'GGCC_C Cuts at: 0 356 764 2213 2490 Size: 356 408 1449 277 Fragments arranged by size: 1449 408 356 277 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2419 2451 2490 Size: 2419 32 39 Fragments arranged by size: 2419 39 32 PstI C_TGCA'G Cuts at: 0 206 377 743 773 803 860 998 2490 Size: 206 171 366 30 30 57 138 1492 Fragments arranged by size: 1492 366 206 171 138 57 30 30 PvuII CAG'CTG Cuts at: 0 363 372 417 777 861 2238 2490 Size: 363 9 45 360 84 1377 252 Fragments arranged by size: 1377 363 360 252 84 45 9 SacI G_AGCT'C Cuts at: 0 1721 2071 2120 2490 Size: 1721 350 49 370 Fragments arranged by size: 1721 370 350 49 SacII CC_GC'GG Cuts at: 0 573 2490 Size: 573 1917 SanDI GG'GwC_CC Cuts at: 0 1528 2490 Size: 1528 962 SbfI CC_TGCA'GG Cuts at: 0 206 998 2490 Size: 206 792 1492 Fragments arranged by size: 1492 792 206 SfcI C'TryA_G Cuts at: 0 202 373 739 769 799 856 994 2490 Size: 202 171 366 30 30 57 138 1496 Fragments arranged by size: 1496 366 202 171 138 57 30 30 SfiI GGCCn_nnn'nGGCC Cuts at: 0 355 1441 1804 2490 Size: 355 1086 363 686 Fragments arranged by size: 1086 686 363 355 SgrAI Cr'CCGG_yG Cuts at: 0 1513 2490 Size: 1513 977 SmaI CCC'GGG Cuts at: 0 274 474 2490 Size: 274 200 2016 Fragments arranged by size: 2016 274 200 SmlI C'TyrA_G Cuts at: 0 1578 2490 Size: 1578 912 StuI AGG'CCT Cuts at: 0 964 2276 2490 Size: 964 1312 214 Fragments arranged by size: 1312 964 214 StyI C'CwwG_G Cuts at: 0 1256 1439 1919 1925 2258 2490 Size: 1256 183 480 6 333 232 Fragments arranged by size: 1256 480 333 232 183 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2021 2403 2490 Size: 2021 382 87 Fragments arranged by size: 2021 382 87 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1675 2490 Size: 1675 815 XmaI C'CCGG_G Cuts at: 0 272 472 2490 Size: 272 200 2018 Fragments arranged by size: 2018 272 200 XmnI GAAnn'nnTTC Cuts at: 0 190 2431 2490 Size: 190 2241 59 Fragments arranged by size: 2241 190 59 Enzymes that do cut: AarI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PciI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI SacII SanDI SbfI SfcI SfiI SgrAI SmaI SmlI StuI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AhdI AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BlpI BmtI BsaBI BsiWI BspHI BsrGI BssHII BstBI BstZ17I BtgZI CspCI DraI EagI EciI EcoRV FalI FseI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI PacI PasI PflMI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SalI SapI ScaI SexAI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI