(Circular) (Six-base) MAPSORT of: pFN21AE9527.seq Check: 8577 from: 1 to: 5896 pFN21AE9527 5896 bp With 182 enzymes: SgfI * August 25, 2016 14:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2247 3239 1937 Size: 310 992 4594 Fragments arranged by size: 4594 992 310 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3959 5343 5611 1632 Size: 2327 1384 268 1917 Fragments arranged by size: 2327 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 2478 2718 4795 5772 1203 Size: 1275 240 2077 977 1327 Fragments arranged by size: 2077 1327 1275 977 240 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 2255 1166 Size: 1089 4807 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5896 AlwNI CAG_nnn'CTG Cuts at: 2783 3639 5211 2783 Size: 856 1572 3468 Fragments arranged by size: 3468 1572 856 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 3205 3431 3583 5692 1493 Size: 1712 226 152 2109 1697 Fragments arranged by size: 2109 1712 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3221 1870 Size: 73 1278 4545 Fragments arranged by size: 4545 1278 73 BamHI G'GATC_C Cuts at: 3226 3593 3226 Size: 367 5529 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 3217 4672 618 Size: 325 217 627 288 1142 1455 1842 Fragments arranged by size: 1842 1455 1142 627 325 288 217 BanII G_rGCy'C Cuts at: 1391 2015 3215 1391 Size: 624 1200 4072 Fragments arranged by size: 4072 1200 624 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 4600 Fragments arranged by size: 4600 971 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4087 4121 1903 Size: 34 50 34 2066 34 3678 Fragments arranged by size: 3678 2066 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3130 3788 4998 3130 Size: 658 1210 4028 Fragments arranged by size: 4028 1210 658 BclI T'GATC_A Cuts at: 1548 2799 1548 Size: 1251 4645 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2354 4507 136 Size: 107 122 71 631 605 682 2153 1525 Fragments arranged by size: 2153 1525 682 631 605 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 2553 3957 5113 1790 Size: 763 1404 1156 2573 Fragments arranged by size: 2573 1404 1156 763 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 5068 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3627 4585 442 Size: 416 53 83 399 24 2210 958 1753 Fragments arranged by size: 2210 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2054 1056 Size: 998 4898 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2539 3127 4556 1239 Size: 1300 588 1429 2579 Fragments arranged by size: 2579 1429 1300 588 Bpu10I CC'TnA_GC Cuts at: 2697 2697 Size: 5896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 4886 5184 4021 Size: 865 298 4733 Fragments arranged by size: 4733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4559 882 Size: 617 3060 2219 Fragments arranged by size: 3060 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 5890 5890 Size: 5896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3703 4085 4720 275 Size: 53 83 186 936 2170 382 635 1451 Fragments arranged by size: 2170 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4329 4727 5001 5148 5559 4329 Size: 398 274 147 411 4666 Fragments arranged by size: 4666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 1178 Size: 30 165 30 748 30 4893 Fragments arranged by size: 4893 748 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2360 1186 Size: 1174 4722 BseYI C'CCAG_C Cuts at: 2015 2032 2764 5099 5595 2015 Size: 17 732 2335 496 2316 Fragments arranged by size: 2335 2316 732 496 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 1163 Size: 573 801 4522 Fragments arranged by size: 4522 801 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3346 4107 5135 1540 Size: 275 186 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3957 4042 5113 3957 Size: 85 1071 4740 Fragments arranged by size: 4740 1071 85 BsmI GAATG_Cn' Cuts at: 2811 3407 3500 2811 Size: 596 93 5207 Fragments arranged by size: 5207 596 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2553 3215 3957 4042 5113 1391 Size: 399 225 538 662 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 662 538 399 225 85 BspEI T'CCGG_A Cuts at: 5559 5559 Size: 5896 BspHI T'CATG_A Cuts at: 2499 2683 3785 5515 2499 Size: 184 1102 1730 2880 Fragments arranged by size: 2880 1730 1102 184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2513 3251 844 Size: 1669 738 3489 Fragments arranged by size: 3489 1669 738 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 4391 4565 65 Size: 1183 1323 1820 174 1396 Fragments arranged by size: 1820 1396 1323 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4540 4727 1700 Size: 110 2730 187 2869 Fragments arranged by size: 2869 2730 187 110 BsrGI T'GTAC_A Cuts at: 96 2979 96 Size: 2883 3013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 2573 794 Size: 1779 4117 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 2617 1184 Size: 1433 4463 BstXI CCAn_nnnn'nTGG Cuts at: 2150 2150 Size: 5896 BstYI r'GATC_y Cuts at: 1972 2096 3226 3593 3978 3995 5447 5533 5545 Size: 124 1130 367 385 17 1452 86 12 Cuts at: 5545 5891 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1130 385 367 346 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2544 2823 513 Size: 552 1479 279 3586 Fragments arranged by size: 3586 1479 552 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 2077 3412 4206 4226 2077 Size: 1335 794 20 3747 Fragments arranged by size: 3747 1335 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3029 4903 809 Size: 507 1713 1874 1802 Fragments arranged by size: 1874 1802 1713 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2346 2742 2883 Size: 54 1425 50 275 18 516 396 141 Cuts at: 2883 3343 4232 5861 8 Size: 460 889 1629 43 Fragments arranged by size: 1629 1425 889 516 460 396 275 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2501 2846 3169 3826 1353 Size: 1148 345 323 657 3423 Fragments arranged by size: 3423 1148 657 345 323 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2539 3127 3959 4556 5343 5611 1239 Size: 393 907 588 832 597 787 268 1524 Fragments arranged by size: 1524 907 832 787 597 588 393 268 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2123 2155 2357 2389 2421 Size: 32 135 32 551 32 202 32 32 Cuts at: 2421 2819 2851 4537 4569 4611 4643 1373 Size: 398 32 1686 32 42 32 2626 Fragments arranged by size: 2626 1686 551 398 202 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 2241 2253 3495 2241 Size: 12 1242 4642 Fragments arranged by size: 4642 1242 12 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5010 5194 1305 Size: 3705 184 2007 Fragments arranged by size: 3705 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2348 2744 2885 10 Size: 54 1425 343 516 396 141 3021 Fragments arranged by size: 3021 1425 516 396 343 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2412 2970 3854 4213 4372 518 Size: 648 1089 157 558 884 359 159 2042 Fragments arranged by size: 2042 1089 884 648 558 359 159 157 MspA1I CmG'CkG Cuts at: 1606 3015 3312 3989 5137 5382 1606 Size: 1409 297 677 1148 245 2120 Fragments arranged by size: 2120 1409 1148 677 297 245 NcoI C'CATG_G Cuts at: 513 1065 2544 2823 513 Size: 552 1479 279 3586 Fragments arranged by size: 3586 1479 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NheI G'CTAG_C Cuts at: 1052 2050 1052 Size: 998 4898 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2482 2722 3254 4799 1132 Size: 97 1253 240 532 1545 2229 Fragments arranged by size: 2229 1545 1253 532 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 2478 2718 4795 2478 Size: 240 2077 3579 Fragments arranged by size: 3579 2077 240 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5896 PfoI T'CCnGG_A Cuts at: 2555 3143 2555 Size: 588 5308 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2228 1613 Size: 615 5281 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 2072 3248 830 Size: 1242 1176 3478 Fragments arranged by size: 3478 1242 1176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 PvuII CAG'CTG Cuts at: 3015 3015 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 4144 5856 4144 Size: 1712 4184 SfcI C'TryA_G Cuts at: 826 1045 2068 3244 4379 5060 5251 826 Size: 219 1023 1176 1135 681 191 1471 Fragments arranged by size: 1471 1176 1135 1023 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2354 2354 Size: 5896 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4000 4901 5163 820 Size: 197 926 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 3254 1132 Size: 2122 3774 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5896 StyI C'CwwG_G Cuts at: 513 1065 2083 2358 2544 2823 3335 513 Size: 552 1018 275 186 279 512 3074 Fragments arranged by size: 3074 1018 552 512 279 275 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3923 3940 4093 4278 4723 325 Size: 1255 2343 17 153 185 445 1498 Fragments arranged by size: 2343 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 2979 4142 Size: 274 80 33 51 1443 504 498 1163 Cuts at: 4142 5854 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1163 504 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5856 5888 5856 Size: 32 5864 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5896 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 4025 4025 Size: 5896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI