Restriction map for
pFN21AB6626
(Circular) (Six-base) MAPSORT of: pFN21AB6626.seq Check: 5570 from: 1 to: 6022
pFN21AB6626 6022 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2949 2949
Size: 6022
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3365 1937
Size: 1428 4594
Acc65I G'GTAC_C
Cuts at: 2322 3343 2322
Size: 1021 5001
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2198 2348 4085 5469 5737 1632
Size: 461 105 150 1737 1384 268 1917
Fragments arranged by size:
1917 1737 1384 461 268 150 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 2867 1166
Size: 1701 4321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3156 1184
Size: 1972 4050
AlwNI CAG_nnn'CTG
Cuts at: 3082 3765 5337 3082
Size: 683 1572 3767
Fragments arranged by size:
3767 1572 683
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 3331 3557 3709 5818 1493
Size: 1838 226 152 2109 1697
Fragments arranged by size:
2109 1838 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3243 4576 160
Size: 872 2211 1333 1606
Fragments arranged by size:
2211 1606 1333 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 3347 1870
Size: 73 1143 261 4545
Fragments arranged by size:
4545 1143 261 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2357 2390 2357
Size: 33 5989
BamHI G'GATC_C
Cuts at: 2008 2619 3352 3719 2008
Size: 611 733 367 4311
Fragments arranged by size:
4311 733 611 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2211 2322 3052 3343 4798
Size: 325 217 627 424 111 730 291 1455
Cuts at: 4798 618
Size: 1842
Fragments arranged by size:
1842 1455 730 627 424 325 291 217
111
BanII G_rGCy'C
Cuts at: 1391 2675 2778 2988 3321 3341 1391
Size: 1284 103 210 333 20 4072
Fragments arranged by size:
4072 1284 333 210 103 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5051
BbvCI CC'TCA_GC
Cuts at: 2414 2414
Size: 6022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2704 4633 136
Size: 107 122 71 631 605 1032 1929 1525
Fragments arranged by size:
1929 1525 1032 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2559 6017 2559
Size: 3458 2564
BlpI GC'TnA_GC
Cuts at: 2588 3439 2588
Size: 851 5171
Bme1580I G_kGCm'C
Cuts at: 1790 4083 5239 1790
Size: 2293 1156 2573
Fragments arranged by size:
2573 2293 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3753 4711 442
Size: 416 53 83 399 24 2336 958 1753
Fragments arranged by size:
2336 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 2735 3059 4682 1239
Size: 1388 108 324 1623 2579
Fragments arranged by size:
2579 1623 1388 324 108
Bpu10I CC'TnA_GC
Cuts at: 2414 2579 2982 2414
Size: 165 403 5454
Fragments arranged by size:
5454 403 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 3218 4147 5012 5310 2942
Size: 276 929 865 298 3654
Fragments arranged by size:
3654 929 865 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4685 882
Size: 617 3186 2219
Fragments arranged by size:
3186 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2350 2599 3813 493
Size: 711 1146 249 1214 2702
Fragments arranged by size:
2702 1214 1146 711 249
BsaBI GATnn'nnATC
Cuts at: 6016 6016
Size: 6022
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4211 4846 275
Size: 53 83 186 936 2296 382 635 1451
Fragments arranged by size:
2296 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2847 4455 4853 5127 5274 5685 2847
Size: 1608 398 274 147 411 3184
Fragments arranged by size:
3184 1608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 1178
Size: 30 165 30 990 30 4777
Fragments arranged by size:
4777 990 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3104 1186
Size: 1918 4104
BseYI C'CCAG_C
Cuts at: 2288 2614 3311 5225 5721 2288
Size: 326 697 1914 496 2589
Fragments arranged by size:
2589 1914 697 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 2903 2921 3126 3152 1163
Size: 573 627 540 18 205 26 4033
Fragments arranged by size:
4033 627 573 540 205 26 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3472 4233 5261 1540
Size: 275 186 1471 761 1028 2301
Fragments arranged by size:
2301 1471 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 2637 4083 4168 5239 2598
Size: 39 1446 85 1071 3381
Fragments arranged by size:
3381 1446 1071 85 39
BsmI GAATG_Cn'
Cuts at: 2286 3533 3626 2286
Size: 1247 93 4682
Fragments arranged by size:
4682 1247 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2598 2637 2675 2778 2988 3321 3341
Size: 399 808 39 38 103 210 333 20
Cuts at: 3341 4083 4168 5239 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 399 333 210 103
85 39 38 20
BspEI T'CCGG_A
Cuts at: 2847 5685 2847
Size: 2838 3184
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2949 3058 3210 3377 844
Size: 2105 109 152 167 3489
Fragments arranged by size:
3489 2105 167 152 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 3909 1394
Size: 429 191 1895 3507
Fragments arranged by size:
3507 1895 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4666 4853 1700
Size: 110 2856 187 2869
Fragments arranged by size:
2869 2856 187 110
BsrGI T'GTAC_A
Cuts at: 96 2478 96
Size: 2382 3640
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 4082 5094 4082
Size: 1012 5010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstXI CCAn_nnnn'nTGG
Cuts at: 2729 2729
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 2008 2559 2619 3352 3719 4104 4121 5573
Size: 36 551 60 733 367 385 17 1452
Cuts at: 5573 5659 5671 6017 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 733 551 385 367 346 86
60 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2383 2702 513
Size: 552 939 379 319 3833
Fragments arranged by size:
3833 939 552 379 319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2188 487
Size: 683 56 962 4321
Fragments arranged by size:
4321 962 683 56
BtsI GCAGTG_nn'
Cuts at: 3062 3538 4332 4352 3062
Size: 476 794 20 4732
Fragments arranged by size:
4732 794 476 20
ClaI AT'CG_AT
Cuts at: 1554 2088 1554
Size: 534 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DraIII CAC_nnn'GTG
Cuts at: 3149 3149
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2856 3222 3469
Size: 54 1425 50 275 18 1026 366 247
Cuts at: 3469 4358 5987 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1026 889 366 275 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 2663 3952 1353
Size: 1175 135 1289 3423
Fragments arranged by size:
3423 1289 1175 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2266 4609 4983 5129 387
Size: 1064 138 677 2343 374 146 1280
Fragments arranged by size:
2343 1280 1064 677 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2198 2348 2627 2735 3059 4085
Size: 393 461 105 150 279 108 324 1026
Cuts at: 4085 4682 5469 5737 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1026 787 597 461 393 324 279
268 150 108 105
EcoO109I rG'GnC_Cy
Cuts at: 3070 3070
Size: 6022
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 5169 1678
Size: 3491 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 4663 4695 4737
Size: 32 135 32 428 32 2631 32 42
Cuts at: 4737 4769 1373
Size: 32 2626
Fragments arranged by size:
2631 2626 428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 2326 3347 2326
Size: 1021 5001
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2516 5136 5320 1305
Size: 1211 2620 184 2007
Fragments arranged by size:
2620 2007 1211 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4200
Fragments arranged by size:
4200 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 3980 4339 4498 518
Size: 648 1701 1113 359 159 2042
Fragments arranged by size:
2042 1701 1113 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2418 2614 3315 3438 4115 5263 5508
Size: 465 347 196 701 123 677 1148 245
Cuts at: 5508 1606
Size: 2120
Fragments arranged by size:
2120 1148 701 677 465 347 245 196
123
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4531
Fragments arranged by size:
4531 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 3380 4925 1132
Size: 97 2151 1545 2229
Fragments arranged by size:
2229 2151 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 2350 3019 1108
Size: 1113 129 669 4111
Fragments arranged by size:
4111 1113 669 129
PfoI T'CCnGG_A
Cuts at: 2751 2874 2970 2751
Size: 123 96 5803
Fragments arranged by size:
5803 123 96
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PmlI CAC'GTG
Cuts at: 2599 2599
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2122 4091 4123 5604 5636 2090
Size: 32 1969 32 1481 32 2476
Fragments arranged by size:
2476 1969 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3070 3070
Size: 6022
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PstI C_TGCA'G
Cuts at: 830 2028 2745 3069 3374 830
Size: 1198 717 324 305 3478
Fragments arranged by size:
3478 1198 717 324 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
PvuII CAG'CTG
Cuts at: 2071 2418 2614 2071
Size: 347 196 5479
Fragments arranged by size:
5479 347 196
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SapI GCTCTTCn'nnn_
Cuts at: 2663 2663
Size: 6022
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SexAI A'CCwGG_T
Cuts at: 3099 3099
Size: 6022
SfcI C'TryA_G
Cuts at: 826 1045 2024 2741 3065 3370 4505 5186 5377
Size: 219 979 717 324 305 1135 681 191
Cuts at: 5377 826
Size: 1471
Fragments arranged by size:
1471 1135 979 717 681 324 305 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 3349 1872
Size: 1477 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2957 3233 4126 5027 5289 820
Size: 197 926 1014 276 893 901 262 1553
Fragments arranged by size:
1553 1014 926 901 893 276 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 5065
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 3213 3283 3461 513
Size: 552 939 36 1173 70 178 3074
Fragments arranged by size:
3074 1173 939 552 178 70 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2879 4049 4066 4219 4404 4849 325
Size: 1255 1299 1170 17 153 185 445 1498
Fragments arranged by size:
1498 1299 1255 1170 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2454 2478 2788
Size: 274 80 33 51 1443 477 24 310
Cuts at: 2788 2892 2925 4268 5980 96
Size: 104 33 1343 1712 138
Fragments arranged by size:
1712 1443 1343 477 310 274 138 104
80 51 33 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 5982 6014 3054
Size: 32 2896 32 3062
Fragments arranged by size:
3062 2896 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 3175 3271 2866
Size: 309 96 5617
Fragments arranged by size:
5617 309 96
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 3347 1870
Size: 1477 4545
XmnI GAAnn'nnTTC
Cuts at: 2561 4151 2561
Size: 1590 4432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI