################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvrII BamHI BbeI BbrPI BbvCI BciVI BfrI BglI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 9 GGTACC G^GTACC AlwNI 365 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 9 GGTACC G^GTACC AspHI 715 GAGCTC G[AT]GC[AT]^C AviII 137 TGCGCA TGC^GCA BalI 92 TGGCCA TGG^CCA BcgI 798 CGAGATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 599 TGATCA T^GATCA BcnI 6 CCGGG CC^[GC]GG BfaI 79 CTAG C^TAG BlpI 808 GCTGAGC GC^T[ACGT]AGC Bpu1102I 808 GCTGAGC GC^T[ACGT]AGC BsaAI 739 TACGTG [CT]AC^GT[GA] BseRI 64 CTCCTC GAGGAG(10/8) BsgI 590 GTGCAG GTGCAG(16/14) BsiHKAI 715 GAGCTC G[AT]GC[AT]^C BsmAI 82 GAGAC GTCTC(1/5) BsmBI 82 GAGACG CGTCTC(1/5) BspHI 719 TCATGA T^CATGA BspMI 586 GCAGGT ACCTGC(4/8) BsrFI 150 GCCGGT [GA]^CCGG[CT] BstXI 218 CCACCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 808 GCTGAGC GC^T[ACGT]AGC Cfr10I 150 GCCGGT [GA]^CCGG[CT] EagI 29 CGGCCG C^GGCCG EclXI 29 CGGCCG C^GGCCG Eco52I 29 CGGCCG C^GGCCG EcoT14I 423 CCTTGG C^C[AT][AT]GG FbaI 599 TGATCA T^GATCA FspI 137 TGCGCA TGC^GCA KpnI 9 GGTACC GGTAC^C KspI 246 CCGCGG CCGC^GG MaeI 79 CTAG C^TAG MaeII 740 ACGT A^CGT MluNI 92 TGGCCA TGG^CCA MscI 92 TGGCCA TGG^CCA NciI 6 CCGGG CC^[GC]GG PleI 769 GAGTC GAGTC(4/5) PvuII 359 CAGCTG CAG^CTG RcaI 719 TCATGA T^CATGA SacI 715 GAGCTC GAGCT^C SacII 246 CCGCGG CCGC^GG StuI 440 AGGCCT AGG^CCT StyI 423 CCTTGG C^C[AT][AT]GG XbaI 78 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 27 CGCG CG^CG 247 CGCG BbsI 855 GAAGAC GAAGAC(2/6) 892 GAAGAC BglII 458 AGATCT A^GATCT 800 AGATCT BpuAI 855 GAAGAC GAAGAC(2/6) 892 GAAGAC BsiEI 29 CGGCCG CG[GA][CT]^CG 260 CGGTCG BsmFI 110 GTCCC GGGAC(10/14) 265 GTCCC BsrDI 375 CATTGC GCAATG(2/0) 305 GCAATG BsrI 695 ACTGG ACTGG(1/-1) 705 ACTGG BstEII 153 GGTGACC G^GT[ACGT]ACC 228 GGTCACC BstPI 153 GGTGACC G^GT[ACGT]ACC 228 GGTCACC BstUI 27 CGCG CG^CG 247 CGCG BstYI 458 AGATCT [GA]^GATC[CT] 800 AGATCT CfoI 26 GCGC GCG^C 138 GCGC DdeI 22 CTGAG C^T[ACGT]AG 809 CTGAG EaeI 29 CGGCCG [CT]^GGCC[GA] 92 TGGCCA EcoO65I 153 GGTGACC G^GT[ACGT]ACC 228 GGTCACC FauI 72 CCCGC CCCGC(4/6) 187 CCCGC HapII 6 CCGG C^CGG 151 CCGG HhaI 26 GCGC GCG^C 138 GCGC HinP1I 26 GCGC G^CGC 138 GCGC HpaII 6 CCGG C^CGG 151 CCGG MflI 458 AGATCT [GA]^GATC[CT] 800 AGATCT MseI 482 TTAA T^TAA 827 TTAA MslI 141 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 664 CACATCAATG MspI 6 CCGG C^CGG 151 CCGG MvnI 27 CGCG CG^CG 247 CGCG PstI 200 CTGCAG CTGCA^G 362 CTGCAG TfiI 745 GATTC G^A[AT]TC 922 GATTC Tru9I 482 TTAA T^TAA 827 TTAA XhoII 458 AGATCT [GA]^GATC[CT] 800 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BanII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DpnII DsaI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI