(Circular) (Six-base) MAPSORT of: pFN21AE6327.seq Check: 3542 from: 1 to: 5647 pFN21AE6327 5647 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2582 2582 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2014 2968 2014 Size: 954 4693 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2789 3710 5094 5362 1632 Size: 1157 921 1384 268 1917 Fragments arranged by size: 1917 1384 1157 921 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 2150 1166 Size: 984 4663 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2168 2432 1184 Size: 984 264 4399 Fragments arranged by size: 4399 984 264 AlwNI CAG_nnn'CTG Cuts at: 2375 3390 4962 2375 Size: 1015 1572 3060 Fragments arranged by size: 3060 1572 1015 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2006 2039 2006 Size: 33 5614 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2152 2315 2968 4423 Size: 325 217 627 227 138 163 653 1455 Cuts at: 4423 618 Size: 1842 Fragments arranged by size: 1842 1455 653 627 325 227 217 163 138 BanII G_rGCy'C Cuts at: 1391 2307 2634 2668 2724 2966 1391 Size: 916 327 34 56 242 4072 Fragments arranged by size: 4072 916 327 242 56 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2867 2904 928 Size: 971 968 37 3671 Fragments arranged by size: 3671 971 968 37 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2792 2826 3838 3872 1903 Size: 34 855 34 1012 34 3678 Fragments arranged by size: 3678 1012 855 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 2604 1548 Size: 1056 4591 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2463 2805 5642 2463 Size: 342 2837 2468 Fragments arranged by size: 2837 2468 342 BlpI GC'TnA_GC Cuts at: 2814 3064 2814 Size: 250 5397 Bme1580I G_kGCm'C Cuts at: 1790 3708 4864 1790 Size: 1918 1156 2573 Fragments arranged by size: 2573 1918 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2497 2497 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 2092 2722 2799 4307 1239 Size: 808 45 630 77 1508 2579 Fragments arranged by size: 2579 1508 808 630 77 45 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2746 3438 493 Size: 711 1542 692 2702 Fragments arranged by size: 2702 1542 711 692 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 3836 4471 275 Size: 53 83 186 936 1921 382 635 1451 Fragments arranged by size: 1921 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 1186 Size: 874 4773 BseYI C'CCAG_C Cuts at: 2306 2405 2435 2818 4850 5346 2306 Size: 99 30 383 2032 496 2607 Fragments arranged by size: 2607 2032 496 383 99 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2616 1163 Size: 573 880 4194 Fragments arranged by size: 4194 880 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2268 3097 3858 4886 1540 Size: 275 186 36 231 829 761 1028 2301 Fragments arranged by size: 2301 1028 829 761 275 231 186 36 BsiHKAI G_wGCw'C Cuts at: 2724 3708 3793 4864 2724 Size: 984 85 1071 3507 Fragments arranged by size: 3507 1071 984 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 BsmBI CGTCTCn'nnnn_ Cuts at: 2081 2081 Size: 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2634 2668 2724 2966 3708 3793 Size: 399 517 327 34 56 242 742 85 Cuts at: 3793 4864 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 327 242 85 56 34 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 2724 3536 5266 2724 Size: 812 1730 3105 Fragments arranged by size: 3105 1730 812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2582 3002 844 Size: 1738 420 3489 Fragments arranged by size: 3489 1738 420 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2317 2379 4142 4316 65 Size: 1183 1069 62 1763 174 1396 Fragments arranged by size: 1763 1396 1183 1069 174 62 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 4291 4478 1700 Size: 110 345 2136 187 2869 Fragments arranged by size: 2869 2136 345 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 2213 2375 794 Size: 1346 73 162 4066 Fragments arranged by size: 4066 1346 162 73 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 2158 2233 1184 Size: 974 75 4598 Fragments arranged by size: 4598 974 75 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2463 2805 2977 3344 3729 3746 5198 5284 Size: 491 342 172 367 385 17 1452 86 Cuts at: 5284 5296 5642 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 491 385 367 346 342 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2148 2251 2865 513 Size: 552 1083 103 614 3295 Fragments arranged by size: 3295 1083 614 552 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 2416 2898 3163 3957 3977 2416 Size: 482 265 794 20 4086 Fragments arranged by size: 4086 794 482 265 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2097 3094 Size: 54 1425 50 275 18 204 63 997 Cuts at: 3094 3983 5612 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 997 889 275 204 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 3094 1537 Size: 275 222 1060 4090 Fragments arranged by size: 4090 1060 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 3577 1353 Size: 2224 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2722 2722 Size: 5647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2092 2722 2789 2799 3710 4307 Size: 393 415 45 630 67 10 911 597 Cuts at: 4307 5094 5362 1239 Size: 787 268 1524 Fragments arranged by size: 1524 911 787 630 597 415 393 268 67 45 10 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 2144 4153 2144 Size: 2009 3638 FspAI rTGC'GCAy Cuts at: 2144 2144 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 4794 1678 Size: 3116 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2918 2950 4288 4320 4362 Size: 32 135 32 1346 32 1338 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 1346 1338 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2018 2972 2018 Size: 954 4693 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2662 4761 4945 1305 Size: 1357 2099 184 2007 Fragments arranged by size: 2099 2007 1357 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 10 Size: 54 1425 343 267 3558 Fragments arranged by size: 3558 1425 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2673 3605 3964 4123 518 Size: 648 984 523 932 359 159 2042 Fragments arranged by size: 2042 984 932 648 523 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2253 2366 3063 3740 4888 5133 1606 Size: 506 141 113 697 677 1148 245 2120 Fragments arranged by size: 2120 1148 697 677 506 245 141 113 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5095 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5647 PfoI T'CCnGG_A Cuts at: 2577 2577 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PstI C_TGCA'G Cuts at: 830 2209 2371 2999 830 Size: 1379 162 628 3478 Fragments arranged by size: 3478 1379 628 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2366 2366 Size: 5647 SacI G_AGCT'C Cuts at: 2724 2724 Size: 5647 SacII CC_GC'GG Cuts at: 2254 2254 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2197 2205 2367 2449 2995 4130 4811 Size: 219 1152 8 162 82 546 1135 681 Cuts at: 4811 5002 826 Size: 191 1471 Fragments arranged by size: 1471 1152 1135 681 546 219 191 162 82 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2447 1517 Size: 930 4717 StyI C'CwwG_G Cuts at: 513 1065 2428 3086 513 Size: 552 1363 658 3074 Fragments arranged by size: 3074 1363 658 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 2563 2641 3674 3691 3844 4029 Size: 1255 674 309 78 1033 17 153 185 Cuts at: 4029 4474 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1033 674 445 309 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3893 5605 96 Size: 274 80 33 51 1443 1916 1712 138 Fragments arranged by size: 1916 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2877 2909 5607 5639 2877 Size: 32 2698 32 2885 Fragments arranged by size: 2885 2698 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2083 2983 2083 Size: 900 4747 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI