################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BciVI BfrI BglI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 269 ACGTGT A^C[GA][CT]GT AspI 1498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 926 TGGCCA TGG^CCA BbrPI 268 CACGTG CAC^GTG BbvCI 1570 GCTGAGG CCTCAGC(-5/-2) BcgI 1358 GCACTGCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 558 TGATCA T^GATCA BfaI 1664 CTAG C^TAG BglII 1166 AGATCT A^GATCT BlpI 1258 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1258 GCTGAGC GC^T[ACGT]AGC BsaAI 268 CACGTG [CT]AC^GT[GA] BsiEI 422 CGATCG CG[GA][CT]^CG BsmI 633 GCATTC GAATGC(1/-1) BsrDI 1415 GCAATG GCAATG(2/0) CelII 1258 GCTGAGC GC^T[ACGT]AGC EarI 1162 GAAGAG CTCTTC(1/4) EcoNI 1566 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 558 TGATCA T^GATCA HgaI 45 GACGC GACGC(5/10) Ksp632I 1162 GAAGAG CTCTTC(1/4) MaeI 1664 CTAG C^TAG MaeII 269 ACGT A^CGT MluNI 926 TGGCCA TGG^CCA MscI 926 TGGCCA TGG^CCA NaeI 1263 GCCGGC GCC^GGC NcoI 84 CCATGG C^CATGG NgoMIV 1263 GCCGGC G^CCGGC NspI 1433 ACATGC [GA]CATG^[CT] PflFI 1498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1072 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 268 CACGTG CAC^GTG PmlI 268 CACGTG CAC^GTG PpuMI 953 GGGACCC [GA]G^G[AT]CC[CT] PvuI 422 CGATCG CGAT^CG SacI 831 GAGCTC GAGCT^C SbfI 1011 CCTGCAGG CCTGCA^GG SmaI 215 CCCGGG CCC^GGG Sse8387I 1011 CCTGCAGG CCTGCA^GG TfiI 1094 GAATC G^A[AT]TC Tsp509I 823 AATT ^AATT Tth111I 1498 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1072 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 215 CCCGGG C^CCGGG XmaI 215 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 1302 GTAC GT^AC 1596 GTAC AlwI 1209 GATCC GGATC(4/5) 498 GGATC BbsI 396 GAAGAC GAAGAC(2/6) 208 GTCTTC BpmI 693 CTCCAG CTGGAG(16/14) 570 CTGGAG BpuAI 396 GAAGAC GAAGAC(2/6) 208 GTCTTC BspMI 1010 ACCTGC ACCTGC(4/8) 1584 GCAGGT BsrFI 1263 GCCGGC [GA]^CCGG[CT] 1285 ACCGGC BstXI 41 CCATGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1226 CCACCAATGTGG CfoI 174 GCGC GCG^C 1467 GCGC Cfr10I 1263 GCCGGC [GA]^CCGG[CT] 1285 ACCGGC DsaI 84 CCATGG C^C[GA][CT]GG 1579 CCACGG HhaI 174 GCGC GCG^C 1467 GCGC HinP1I 174 GCGC G^CGC 1467 GCGC MaeIII 354 GTCAC ^GT[ACGT]AC 367 GTCAC PstI 1012 CTGCAG CTGCA^G 1487 CTGCAG RsaI 1302 GTAC GT^AC 1596 GTAC ScaI 1301 AGTACT AGT^ACT 1595 AGTACT SfcI 1012 CTGCAG C^T[GA][CT]AG 1487 CTGCAG Tsp45I 354 GTCAC ^GT[GC]AC 367 GTCAC XcmI 416 CCATCACGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1556 CCAAGTGTGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AluI Alw44I AlwNI ApaLI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI TspRI TthHB8I XhoII