(Circular) (Six-base) MAPSORT of: pFN21AE6432.seq Check: 4177 from: 1 to: 6376 pFN21AE6432 6376 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3580 3580 Size: 6376 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3719 1937 Size: 1782 4594 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 2461 4439 5823 6091 1632 Size: 779 50 1978 1384 268 1917 Fragments arranged by size: 1978 1917 1384 779 268 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 2274 5275 6252 1203 Size: 1071 3001 977 1327 Fragments arranged by size: 3001 1327 1071 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 2186 2429 3236 1166 Size: 1020 243 807 4306 Fragments arranged by size: 4306 1020 807 243 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 1184 Size: 1020 5356 AlwNI CAG_nnn'CTG Cuts at: 2980 3025 3642 4119 5691 2980 Size: 45 617 477 1572 3665 Fragments arranged by size: 3665 1572 617 477 45 ApaLI G'TGCA_C Cuts at: 2338 2863 2879 4433 5589 2338 Size: 525 16 1554 1156 3125 Fragments arranged by size: 3125 1554 1156 525 16 ApoI r'AATT_y Cuts at: 1493 3685 3911 4063 6172 1493 Size: 2192 226 152 2109 1697 Fragments arranged by size: 2192 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2220 2962 3480 3498 3701 1870 Size: 73 121 156 742 518 18 203 4545 Fragments arranged by size: 4545 742 518 203 156 121 73 18 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 3487 3580 3697 5152 Size: 325 217 627 242 1458 93 117 1455 Cuts at: 5152 618 Size: 1842 Fragments arranged by size: 1842 1458 1455 627 325 242 217 117 93 BanII G_rGCy'C Cuts at: 1391 2040 2840 3097 3634 3695 1391 Size: 649 800 257 537 61 4072 Fragments arranged by size: 4072 800 649 537 257 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 2408 928 Size: 971 307 202 4896 Fragments arranged by size: 4896 971 307 202 BbvCI CC'TCA_GC Cuts at: 3576 3576 Size: 6376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3352 3386 4567 4601 1903 Size: 34 1415 34 1181 34 3678 Fragments arranged by size: 3678 1415 1181 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 2563 1548 Size: 1015 5361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3171 6371 3171 Size: 3200 3176 BlpI GC'TnA_GC Cuts at: 3264 3793 3264 Size: 529 5847 Bme1580I G_kGCm'C Cuts at: 1790 2342 2867 2883 3522 4437 5593 1790 Size: 552 525 16 639 915 1156 2573 Fragments arranged by size: 2573 1156 915 639 552 525 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2854 4107 5065 Size: 416 53 83 399 24 1437 1253 958 Cuts at: 5065 442 Size: 1753 Fragments arranged by size: 1753 1437 1253 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2596 2683 5036 1239 Size: 1357 87 2353 2579 Fragments arranged by size: 2579 2353 1357 87 Bpu10I CC'TnA_GC Cuts at: 2034 2839 3576 2034 Size: 805 737 4834 Fragments arranged by size: 4834 805 737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 2255 2657 4501 5366 5664 2126 Size: 129 402 1844 865 298 2838 Fragments arranged by size: 2838 1844 865 402 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2275 4167 493 Size: 711 1071 1892 2702 Fragments arranged by size: 2702 1892 1071 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 2771 2801 1178 Size: 30 165 30 1278 30 60 30 4753 Fragments arranged by size: 4753 1278 165 60 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 2453 2980 1186 Size: 1078 189 527 4582 Fragments arranged by size: 4582 1078 527 189 BseYI C'CCAG_C Cuts at: 2053 2609 2613 3076 3273 3662 5579 6075 2053 Size: 556 4 463 197 389 1917 496 2354 Fragments arranged by size: 2354 1917 556 496 463 389 197 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2966 3051 3537 3628 1163 Size: 573 549 681 85 486 91 3911 Fragments arranged by size: 3911 681 573 549 486 91 85 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2430 3826 4587 5615 1540 Size: 275 186 429 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 429 275 186 BsiHKAI G_wGCw'C Cuts at: 2342 2840 2867 2883 3290 3327 4437 4522 5593 Size: 498 27 16 407 37 1110 85 1071 Cuts at: 5593 2342 Size: 3125 Fragments arranged by size: 3125 1110 1071 498 407 85 37 27 16 BsmI GAATG_Cn' Cuts at: 2638 3887 3980 2638 Size: 1249 93 5034 Fragments arranged by size: 5034 1249 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2342 2840 2867 2883 3097 3290 Size: 399 250 302 498 27 16 214 193 Cuts at: 3290 3327 3522 3634 3695 4437 4522 5593 1391 Size: 37 195 112 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 302 250 214 195 193 112 85 61 37 27 16 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3024 3580 3731 844 Size: 2180 556 151 3489 Fragments arranged by size: 3489 2180 556 151 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3427 4871 5045 65 Size: 1183 2179 1444 174 1396 Fragments arranged by size: 2179 1444 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3268 3290 5020 5207 1700 Size: 110 1458 22 1730 187 2869 Fragments arranged by size: 2869 1730 1458 187 110 22 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 2782 3025 794 Size: 1469 519 243 4145 Fragments arranged by size: 4145 1469 519 243 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstXI CCAn_nnnn'nTGG Cuts at: 2053 3238 2053 Size: 1185 5191 BstYI r'GATC_y Cuts at: 1972 2503 3171 3213 3706 4073 4458 4475 5927 Size: 531 668 42 493 367 385 17 1452 Cuts at: 5927 6013 6025 6371 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 668 531 493 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2089 3584 513 Size: 552 1024 1495 3305 Fragments arranged by size: 3305 1495 1024 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3169 487 Size: 683 56 1943 3694 Fragments arranged by size: 3694 1943 683 56 BtsI GCAGTG_nn' Cuts at: 2168 2792 2943 2983 3039 3363 3892 4686 4706 Size: 624 151 40 56 324 529 794 20 Cuts at: 4706 2168 Size: 3838 Fragments arranged by size: 3838 794 624 529 324 151 56 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2433 2931 3292 Size: 54 1425 50 275 18 603 498 361 Cuts at: 3292 3823 4712 6341 8 Size: 531 889 1629 43 Fragments arranged by size: 1629 1425 889 603 531 498 361 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3162 4306 1353 Size: 1809 1144 3423 Fragments arranged by size: 3423 1809 1144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2615 4963 5337 5483 387 Size: 1064 138 1026 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 1026 374 146 138 EcoICRI GAG'CTC Cuts at: 2838 2838 Size: 6376 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2461 2596 2683 4439 5036 5823 Size: 393 779 50 135 87 1756 597 787 Cuts at: 5823 6091 1239 Size: 268 1524 Fragments arranged by size: 1756 1524 787 779 597 393 268 135 87 50 EcoNI CCTnn'n_nnAGG Cuts at: 3575 3575 Size: 6376 EcoO109I rG'GnC_Cy Cuts at: 2058 2959 3022 3639 2058 Size: 901 63 617 4795 Fragments arranged by size: 4795 901 617 63 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 6376 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 5523 1678 Size: 3845 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 2681 2713 3492 Size: 32 135 32 857 32 220 32 779 Cuts at: 3492 3524 5017 5049 5091 5123 1373 Size: 32 1493 32 42 32 2626 Fragments arranged by size: 2626 1493 857 779 220 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3720 3966 669 Size: 988 281 1782 246 3079 Fragments arranged by size: 3079 1782 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5490 5674 1305 Size: 684 3501 184 2007 Fragments arranged by size: 3501 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2933 10 Size: 54 1425 343 1101 3453 Fragments arranged by size: 3453 1425 1101 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2429 3236 4334 4693 4852 518 Size: 648 1020 243 807 1098 359 159 2042 Fragments arranged by size: 2042 1098 1020 807 648 359 243 159 MspA1I CmG'CkG Cuts at: 1606 2569 2613 2652 3277 3792 4469 5617 5862 Size: 963 44 39 625 515 677 1148 245 Cuts at: 5862 1606 Size: 2120 Fragments arranged by size: 2120 1148 963 677 625 515 245 44 39 NaeI GCC'GGC Cuts at: 3270 3270 Size: 6376 NcoI C'CATG_G Cuts at: 513 1065 2089 513 Size: 552 1024 4800 Fragments arranged by size: 4800 1024 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NgoMIV G'CCGG_C Cuts at: 3268 3268 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3442 3734 5279 1132 Size: 97 2213 292 1545 2229 Fragments arranged by size: 2229 2213 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 2771 3626 1348 Size: 1423 855 4098 Fragments arranged by size: 4098 1423 855 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 3083 1108 Size: 1975 4401 PfoI T'CCnGG_A Cuts at: 2097 2742 2889 2097 Size: 645 147 5584 Fragments arranged by size: 5584 645 147 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PmlI CAC'GTG Cuts at: 2275 2275 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2959 2959 Size: 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PstI C_TGCA'G Cuts at: 830 3021 3496 3728 830 Size: 2191 475 232 3478 Fragments arranged by size: 3478 2191 475 232 PvuI CG_AT'CG Cuts at: 2001 2430 2001 Size: 429 5947 PvuII CAG'CTG Cuts at: 2569 2613 2652 2569 Size: 44 39 6293 Fragments arranged by size: 6293 44 39 SacI G_AGCT'C Cuts at: 2840 2840 Size: 6376 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SanDI GG'GwC_CC Cuts at: 2959 2959 Size: 6376 SbfI CC_TGCA'GG Cuts at: 3021 3728 3021 Size: 707 5669 ScaI AGT'ACT Cuts at: 3308 3602 4624 6336 3308 Size: 294 1022 1712 3348 Fragments arranged by size: 3348 1712 1022 294 SfcI C'TryA_G Cuts at: 826 1045 3017 3492 3724 4859 5540 5731 826 Size: 219 1972 475 232 1135 681 191 1471 Fragments arranged by size: 1972 1471 1135 681 475 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 2222 3703 1872 Size: 350 1481 4545 Fragments arranged by size: 4545 1481 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 2234 2672 4480 5381 5643 Size: 197 926 198 93 438 1808 901 262 Cuts at: 5643 820 Size: 1553 Fragments arranged by size: 1808 1553 926 901 438 262 198 197 93 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2089 2343 2367 3815 513 Size: 552 1024 254 24 1448 3074 Fragments arranged by size: 3074 1448 1024 552 254 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2017 2155 2894 4403 4420 4573 4758 Size: 1255 437 138 739 1509 17 153 185 Cuts at: 4758 5203 325 Size: 445 1498 Fragments arranged by size: 1509 1498 1255 739 445 437 185 153 138 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3306 3600 4622 Size: 274 80 33 51 1443 1329 294 1022 Cuts at: 4622 6334 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1329 1022 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2771 2803 6336 6368 2771 Size: 32 3533 32 2779 Fragments arranged by size: 3533 2779 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3506 1316 Size: 2190 4186 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 3568 2428 Size: 1140 5236 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 2220 3701 1870 Size: 350 1481 4545 Fragments arranged by size: 4545 1481 350 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI