Restriction map for
pFN21AE6432
(Linear) (Six-base) MAPSORT of: KIEE6432.seq Check: 4138 from: 1 to: 1671
KIEE6432.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1575 1671
Size: 1575 96
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 456 1671
Size: 406 50 1215
Fragments arranged by size:
1215 406 50
AflIII A'CryG_T
Cuts at: 0 269 1671
Size: 269 1402
AleI CACnn'nnGTG
Cuts at: 0 181 424 1231 1671
Size: 181 243 807 440
Fragments arranged by size:
807 440 243 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 1671
Size: 199 1472
AlwNI CAG_nnn'CTG
Cuts at: 0 975 1020 1637 1671
Size: 975 45 617 34
Fragments arranged by size:
975 617 45 34
ApaLI G'TGCA_C
Cuts at: 0 333 858 874 1671
Size: 333 525 16 797
Fragments arranged by size:
797 525 333 16
AvaI C'yCGr_G
Cuts at: 0 59 215 957 1475 1493 1671
Size: 59 156 742 518 18 178
Fragments arranged by size:
742 518 178 156 59 18
BanI G'GyrC_C
Cuts at: 0 24 1482 1575 1671
Size: 24 1458 93 96
Fragments arranged by size:
1458 96 93 24
BanII G_rGCy'C
Cuts at: 0 35 835 1092 1629 1671
Size: 35 800 257 537 42
Fragments arranged by size:
800 537 257 42 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 403 1671
Size: 201 202 1268
Fragments arranged by size:
1268 202 201
BbvCI CC'TCA_GC
Cuts at: 0 1571 1671
Size: 1571 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1347 1381 1671
Size: 1347 34 290
Fragments arranged by size:
1347 290 34
BclI T'GATC_A
Cuts at: 0 558 1671
Size: 558 1113
BglII A'GATC_T
Cuts at: 0 1166 1671
Size: 1166 505
BlpI GC'TnA_GC
Cuts at: 0 1259 1671
Size: 1259 412
Bme1580I G_kGCm'C
Cuts at: 0 337 862 878 1517 1671
Size: 337 525 16 639 154
Fragments arranged by size:
639 525 337 154 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 849 1671
Size: 849 822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 678 1671
Size: 591 87 993
Fragments arranged by size:
993 591 87
Bpu10I CC'TnA_GC
Cuts at: 0 29 834 1571 1671
Size: 29 805 737 100
Fragments arranged by size:
805 737 100 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 250 652 1671
Size: 121 129 402 1019
Fragments arranged by size:
1019 402 129 121
BsaAI yAC'GTr
Cuts at: 0 270 1671
Size: 270 1401
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 766 796 1671
Size: 676 30 60 30 875
Fragments arranged by size:
875 676 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 448 975 1671
Size: 259 189 527 696
Fragments arranged by size:
696 527 259 189
BseYI C'CCAG_C
Cuts at: 0 48 604 608 1071 1268 1657 1671
Size: 48 556 4 463 197 389 14
Fragments arranged by size:
556 463 389 197 48 14 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 961 1046 1532 1623 1671
Size: 280 681 85 486 91 48
Fragments arranged by size:
681 486 280 91 85 48
BsiEI CG_ry'CG
Cuts at: 0 425 1671
Size: 425 1246
BsiHKAI G_wGCw'C
Cuts at: 0 337 835 862 878 1285 1322 1671
Size: 337 498 27 16 407 37 349
Fragments arranged by size:
498 407 349 337 37 27 16
BsmI GAATG_Cn'
Cuts at: 0 633 1671
Size: 633 1038
Bsp1286I G_dGCh'C
Cuts at: 0 35 337 835 862 878 1092 1285 1322
Size: 35 302 498 27 16 214 193 37
Cuts at: 1322 1517 1629 1671
Size: 195 112 42
Fragments arranged by size:
498 302 214 195 193 112 42 37
35 27 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1019 1575 1671
Size: 1019 556 96
Fragments arranged by size:
1019 556 96
BsrDI GCAATG_nn'
Cuts at: 0 1422 1671
Size: 1422 249
BsrFI r'CCGG_y
Cuts at: 0 1263 1285 1671
Size: 1263 22 386
Fragments arranged by size:
1263 386 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 777 1020 1671
Size: 258 519 243 651
Fragments arranged by size:
651 519 258 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1233 1671
Size: 48 1185 438
Fragments arranged by size:
1185 438 48
BstYI r'GATC_y
Cuts at: 0 498 1166 1208 1671
Size: 498 668 42 463
Fragments arranged by size:
668 498 463 42
BtgI C'CryG_G
Cuts at: 0 84 1579 1671
Size: 84 1495 92
Fragments arranged by size:
1495 92 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1164 1671
Size: 1164 507
BtsI GCAGTG_nn'
Cuts at: 0 163 787 938 978 1034 1358 1671
Size: 163 624 151 40 56 324 313
Fragments arranged by size:
624 324 313 163 151 56 40
EaeI y'GGCC_r
Cuts at: 0 428 926 1287 1671
Size: 428 498 361 384
Fragments arranged by size:
498 428 384 361
EarI CTCTTCn'nnn_
Cuts at: 0 1157 1671
Size: 1157 514
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 1671
Size: 610 1061
EcoICRI GAG'CTC
Cuts at: 0 833 1671
Size: 833 838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 456 591 678 1671
Size: 406 50 135 87 993
Fragments arranged by size:
993 406 135 87 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1570 1671
Size: 1570 101
EcoO109I rG'GnC_Cy
Cuts at: 0 53 954 1017 1634 1671
Size: 53 901 63 617 37
Fragments arranged by size:
901 617 63 53 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 1671
Size: 726 945
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 676 708 1487 1519 1671
Size: 424 32 220 32 779 32 152
Fragments arranged by size:
779 424 220 152 32 32 32
MscI TGG'CCA
Cuts at: 0 928 1671
Size: 928 743
MslI CAynn'nnrTG
Cuts at: 0 181 424 1231 1671
Size: 181 243 807 440
Fragments arranged by size:
807 440 243 181
MspA1I CmG'CkG
Cuts at: 0 564 608 647 1272 1671
Size: 564 44 39 625 399
Fragments arranged by size:
625 564 399 44 39
NaeI GCC'GGC
Cuts at: 0 1265 1671
Size: 1265 406
NcoI C'CATG_G
Cuts at: 0 84 1671
Size: 84 1587
NgoMIV G'CCGG_C
Cuts at: 0 1263 1671
Size: 1263 408
NspI r_CATG'y
Cuts at: 0 1437 1671
Size: 1437 234
PasI CC'CwG_GG
Cuts at: 0 766 1621 1671
Size: 766 855 50
Fragments arranged by size:
855 766 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 1078 1671
Size: 1078 593
PfoI T'CCnGG_A
Cuts at: 0 92 737 884 1671
Size: 92 645 147 787
Fragments arranged by size:
787 645 147 92
PmlI CAC'GTG
Cuts at: 0 270 1671
Size: 270 1401
PpuMI rG'GwC_Cy
Cuts at: 0 954 1671
Size: 954 717
PstI C_TGCA'G
Cuts at: 0 1016 1491 1671
Size: 1016 475 180
Fragments arranged by size:
1016 475 180
PvuI CG_AT'CG
Cuts at: 0 425 1671
Size: 425 1246
PvuII CAG'CTG
Cuts at: 0 564 608 647 1671
Size: 564 44 39 1024
Fragments arranged by size:
1024 564 44 39
SacI G_AGCT'C
Cuts at: 0 835 1671
Size: 835 836
SanDI GG'GwC_CC
Cuts at: 0 954 1671
Size: 954 717
SbfI CC_TGCA'GG
Cuts at: 0 1016 1671
Size: 1016 655
ScaI AGT'ACT
Cuts at: 0 1303 1597 1671
Size: 1303 294 74
Fragments arranged by size:
1303 294 74
SfcI C'TryA_G
Cuts at: 0 1012 1487 1671
Size: 1012 475 184
Fragments arranged by size:
1012 475 184
SmaI CCC'GGG
Cuts at: 0 217 1671
Size: 217 1454
SmlI C'TyrA_G
Cuts at: 0 136 229 667 1671
Size: 136 93 438 1004
Fragments arranged by size:
1004 438 136 93
StyI C'CwwG_G
Cuts at: 0 84 338 362 1671
Size: 84 254 24 1309
Fragments arranged by size:
1309 254 84 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 150 889 1671
Size: 12 138 739 782
Fragments arranged by size:
782 739 138 12
TatI w'GTAC_w
Cuts at: 0 1301 1595 1671
Size: 1301 294 76
Fragments arranged by size:
1301 294 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 1671
Size: 766 32 873
Fragments arranged by size:
873 766 32
Tth111I GACn'n_nGTC
Cuts at: 0 1501 1671
Size: 1501 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 1563 1671
Size: 423 1140 108
Fragments arranged by size:
1140 423 108
XmaI C'CCGG_G
Cuts at: 0 215 1671
Size: 215 1456
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaLI AvaI
BanI BanII BbsI BbvCI BcgI BclI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PstI PvuI PvuII SacI
SanDI SbfI ScaI SfcI SmaI SmlI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BglI BmgBI BmtI BplI
BsaI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI RsrII SacII SalI SapI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XhoI XmnI ZraI