################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII Cfr10I CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoT14I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 30 CGCG CG^CG AcyI 1057 GGCGCC G[GA]^CG[CT]C AflIII 526 ACATGT A^C[GA][CT]GT Alw44I 944 GTGCAC G^TGCAC AocI 1044 CCTGAGG CC^T[ACGT]AGG ApaLI 944 GTGCAC G^TGCAC AspHI 944 GTGCAC G[AT]GC[AT]^C BamHI 1110 GGATCC G^GATCC BbeI 1057 GGCGCC GGCGC^C BbsI 1001 GAAGAC GAAGAC(2/6) BfaI 802 CTAG C^TAG BpuAI 1001 GAAGAC GAAGAC(2/6) BsaHI 1057 GGCGCC G[GA]^CG[CT]C BsaI 905 GAGACC GGTCTC(1/5) BsiHKAI 944 GTGCAC G[AT]GC[AT]^C BsmAI 905 GAGAC GTCTC(1/5) BsmI 227 GAATGC GAATGC(1/-1) BspDI 118 ATCGAT AT^CGAT BspLU11I 526 ACATGT A^CATGT BsrBI 1102 GAGCGG CCGCTC(-3/-3) BstEII 895 GGTTACC G^GT[ACGT]ACC BstPI 895 GGTTACC G^GT[ACGT]ACC BstUI 30 CGCG CG^CG Bsu36I 1044 CCTGAGG CC^T[ACGT]AGG ClaI 118 ATCGAT AT^CGAT DraI 520 TTTAAA TTT^AAA DraII 760 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 612 CCACGG C^C[GA][CT]GG Eco81I 1044 CCTGAGG CC^T[ACGT]AGG EcoO109I 760 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 895 GGTTACC G^GT[ACGT]ACC EcoRV 160 GATATC GAT^ATC EcoT22I 861 ATGCAT ATGCA^T HgaI 584 GACGC GACGC(5/10) Hin1I 1057 GGCGCC G[GA]^CG[CT]C HincII 530 GTCAAC GT[CT]^[GA]AC HindII 530 GTCAAC GT[CT]^[GA]AC HphI 126 TCACC GGTGA(8/7) KasI 1057 GGCGCC G^GCGCC MaeI 802 CTAG C^TAG MvnI 30 CGCG CG^CG NarI 1057 GGCGCC GG^CGCC NsiI 861 ATGCAT ATGCA^T PflMI 415 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 675 GACTC GAGTC(4/5) PpuMI 760 GGGACCT [GA]G^G[AT]CC[CT] PstI 278 CTGCAG CTGCA^G ScaI 138 AGTACT AGT^ACT SfcI 278 CTGCAG C^T[GA][CT]AG SfoI 1057 GGCGCC GGC^GCC Van91I 415 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 616 GGACC G^G[AT]CC 761 GGACC BcnI 5 CCCGG CC^[GC]GG 83 CCGGG BmyI 543 GTGCCC G[AGT]GC[ACT]^C 944 GTGCAC BpmI 794 CTCCAG CTGGAG(16/14) 42 CTGGAG BsmFI 760 GGGAC GGGAC(10/14) 361 GTCCC Bsp1286I 543 GTGCCC G[AGT]GC[ACT]^C 944 GTGCAC BspMI 745 ACCTGC ACCTGC(4/8) 955 ACCTGC BstYI 345 AGATCC [GA]^GATC[CT] 1110 GGATCC EcoRI 304 GAATTC G^AATTC 471 GAATTC HaeII 13 AGCGCC [GA]GCGC^[CT] 1057 GGCGCC HapII 6 CCGG C^CGG 83 CCGG HpaII 6 CCGG C^CGG 83 CCGG MflI 345 AGATCC [GA]^GATC[CT] 1110 GGATCC MspI 6 CCGG C^CGG 83 CCGG NciI 5 CCCGG CC^[GC]GG 83 CCGGG NspI 526 ACATGT [GA]CATG^[CT] 1038 ACATGC TaqI 119 TCGA T^CGA 846 TCGA TfiI 704 GATTC G^A[AT]TC 1120 GAATC Tsp45I 673 GTGAC ^GT[GC]AC 722 GTGAC TthHB8I 119 TCGA T^CGA 846 TCGA XhoII 345 AGATCC [GA]^GATC[CT] 1110 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanI BbvI BsaJI BseRI BsiYI BslI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I TspRI