(Circular) (Six-base) MAPSORT of: pFN21AE3639.seq Check: 7788 from: 1 to: 5842 pFN21AE3639 5842 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2759 2759 Size: 5842 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3905 5289 5557 1632 Size: 870 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 870 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 2531 4741 5718 1203 Size: 1328 2210 977 1327 Fragments arranged by size: 2210 1328 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 3585 5157 3585 Size: 1572 4270 ApaLI G'TGCA_C Cuts at: 2949 3899 5055 2949 Size: 950 1156 3736 Fragments arranged by size: 3736 1156 950 ApoI r'AATT_y Cuts at: 1493 2266 2309 2476 3151 3377 3529 5638 1493 Size: 773 43 167 675 226 152 2109 1697 Fragments arranged by size: 2109 1697 773 675 226 167 152 43 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 3167 1870 Size: 73 1224 4545 Fragments arranged by size: 4545 1224 73 BamHI G'GATC_C Cuts at: 3115 3172 3539 3115 Size: 57 367 5418 Fragments arranged by size: 5418 367 57 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 2559 3062 3163 4618 Size: 325 217 627 407 365 503 101 1455 Cuts at: 4618 618 Size: 1842 Fragments arranged by size: 1842 1455 627 503 407 365 325 217 101 BanII G_rGCy'C Cuts at: 1391 3161 1391 Size: 1770 4072 BbeI G_GCGC'C Cuts at: 3066 3066 Size: 5842 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3013 928 Size: 971 1114 3757 Fragments arranged by size: 3757 1114 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BfrBI ATG'CAT Cuts at: 2868 2868 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4453 136 Size: 107 122 71 631 605 2781 1525 Fragments arranged by size: 2781 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5837 5837 Size: 5842 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 2552 2953 3903 5059 1790 Size: 762 401 950 1156 2573 Fragments arranged by size: 2573 1156 950 762 401 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3573 4531 442 Size: 416 53 83 399 24 2156 958 1753 Fragments arranged by size: 2156 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2784 4502 1239 Size: 829 716 1718 2579 Fragments arranged by size: 2579 1718 829 716 Bpu10I CC'TnA_GC Cuts at: 3071 3071 Size: 5842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2904 4505 882 Size: 617 1405 1601 2219 Fragments arranged by size: 2219 1601 1405 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3063 3649 4031 4666 Size: 53 83 186 936 1530 586 382 635 Cuts at: 4666 275 Size: 1451 Fragments arranged by size: 1530 1451 936 635 586 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4275 4673 4947 5094 5505 4275 Size: 398 274 147 411 4612 Fragments arranged by size: 4612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 1178 Size: 30 165 30 693 30 4894 Fragments arranged by size: 4894 693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2117 3119 1186 Size: 843 88 1002 3909 Fragments arranged by size: 3909 1002 843 88 BseYI C'CCAG_C Cuts at: 2374 3066 5045 5541 2374 Size: 692 1979 496 2675 Fragments arranged by size: 2675 1979 692 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 4053 5081 1540 Size: 275 186 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2953 3903 3988 5059 2953 Size: 950 85 1071 3736 Fragments arranged by size: 3736 1071 950 85 BsmI GAATG_Cn' Cuts at: 2238 3353 3446 2238 Size: 1115 93 4634 Fragments arranged by size: 4634 1115 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2552 2953 3161 3903 3988 5059 1391 Size: 399 762 401 208 742 85 1071 2174 Fragments arranged by size: 2174 1071 762 742 401 399 208 85 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2759 2969 3197 844 Size: 1915 210 228 3489 Fragments arranged by size: 3489 1915 228 210 BsrBI CCG'CTC Cuts at: 1394 1823 3109 3729 1394 Size: 429 1286 620 3507 Fragments arranged by size: 3507 1286 620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 2900 1184 Size: 1716 4126 BstYI r'GATC_y Cuts at: 1972 2350 3115 3172 3539 3924 3941 5393 5479 Size: 378 765 57 367 385 17 1452 86 Cuts at: 5479 5491 5837 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 765 385 378 367 346 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 3050 3050 Size: 5842 BtgI C'CryG_G Cuts at: 513 1065 2004 2617 513 Size: 552 939 613 3738 Fragments arranged by size: 3738 939 613 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 2072 2825 3010 3358 4152 4172 2072 Size: 753 185 348 794 20 3742 Fragments arranged by size: 3742 794 753 348 185 20 ClaI AT'CG_AT Cuts at: 1554 2124 1554 Size: 570 5272 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 2527 3146 2527 Size: 619 5223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3289 4178 5807 Size: 54 1425 50 275 18 1459 889 1629 Cuts at: 5807 8 Size: 43 Fragments arranged by size: 1629 1459 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3772 1353 Size: 2419 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 4429 4803 4949 387 Size: 1064 138 507 2333 374 146 1280 Fragments arranged by size: 2333 1280 1064 507 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2502 2784 3905 4502 5289 5557 Size: 393 436 434 282 1121 597 787 268 Cuts at: 5557 1239 Size: 1524 Fragments arranged by size: 1524 1121 787 597 436 434 393 282 268 EcoO109I rG'GnC_Cy Cuts at: 2766 2766 Size: 5842 EcoRI G'AATT_C Cuts at: 2309 2476 3151 3529 2309 Size: 167 675 378 4622 Fragments arranged by size: 4622 675 378 167 EcoRV GAT'ATC Cuts at: 2167 2167 Size: 5842 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 2022 3066 4989 1678 Size: 344 1044 1923 2531 Fragments arranged by size: 2531 1923 1044 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 4483 4515 4557 Size: 32 135 32 541 32 2338 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 2338 541 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2537 3186 3432 669 Size: 988 281 599 649 246 3079 Fragments arranged by size: 3079 988 649 599 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KasI G'GCGC_C Cuts at: 3062 3062 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2378 4956 5140 1305 Size: 1073 2578 184 2007 Fragments arranged by size: 2578 2007 1073 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3258 3935 5083 5328 1606 Size: 1652 677 1148 245 2120 Fragments arranged by size: 2120 1652 1148 677 245 NarI GG'CG_CC Cuts at: 3063 3063 Size: 5842 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NsiI A_TGCA'T Cuts at: 2870 2870 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 2535 3047 3200 4745 1132 Size: 97 1306 512 153 1545 2229 Fragments arranged by size: 2229 1545 1306 512 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 2531 4741 2531 Size: 2210 3632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2426 1108 Size: 1318 4524 PfoI T'CCnGG_A Cuts at: 2800 2800 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 2403 3911 3943 5424 5456 2371 Size: 32 1508 32 1481 32 2757 Fragments arranged by size: 2757 1508 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2766 2766 Size: 5842 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PstI C_TGCA'G Cuts at: 830 2287 3194 830 Size: 1457 907 3478 Fragments arranged by size: 3478 1457 907 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 2145 4090 5802 2145 Size: 1945 1712 2185 Fragments arranged by size: 2185 1945 1712 SfcI C'TryA_G Cuts at: 826 1045 2283 3190 4325 5006 5197 826 Size: 219 1238 907 1135 681 191 1471 Fragments arranged by size: 1471 1238 1135 907 681 219 191 SfoI GGC'GCC Cuts at: 3064 3064 Size: 5842 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 3169 1872 Size: 1297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3946 4847 5109 820 Size: 197 926 2003 901 262 1553 Fragments arranged by size: 2003 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 3281 513 Size: 552 939 1277 3074 Fragments arranged by size: 3074 1277 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3869 3886 4039 4224 4669 325 Size: 1255 2289 17 153 185 445 1498 Fragments arranged by size: 2289 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2143 4088 5800 Size: 274 80 33 51 1443 166 1945 1712 Cuts at: 5800 96 Size: 138 Fragments arranged by size: 1945 1712 1443 274 166 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2661 2693 5802 5834 2661 Size: 32 3109 32 2669 Fragments arranged by size: 3109 2669 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 3167 1870 Size: 1297 4545 XmnI GAAnn'nnTTC Cuts at: 3971 3971 Size: 5842 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI