Restriction map for
pFN21AE6235
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 575 CCGC CCGC(-3/-1)
AflIII 238 ACATGT A^C[GA][CT]GT
AlwNI 516 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 360 CCTGAGG CC^T[ACGT]AGG
AvaII 423 GGACC G^G[AT]CC
BanII 304 GAGCTC G[GA]GC[CT]^C
BbsI 73 GTCTTC GAAGAC(2/6)
BfaI 617 CTAG C^TAG
BlpI 301 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 301 GCTGAGC GC^T[ACGT]AGC
BpuAI 73 GTCTTC GAAGAC(2/6)
BsaBI 538 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 19 GAGACC GGTCTC(1/5)
BsmI 670 GCATTC GAATGC(1/-1)
BspHI 339 TCATGA T^CATGA
BspLU11I 238 ACATGT A^CATGT
BsrDI 398 GCAATG GCAATG(2/0)
BstXI 678 CCAGTTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 552 GGATCT [GA]^GATC[CT]
Bsu36I 360 CCTGAGG CC^T[ACGT]AGG
CelII 301 GCTGAGC GC^T[ACGT]AGC
CfoI 435 GCGC GCG^C
Cfr13I 423 GGACC G^G[ACGT]CC
DsaI 253 CCATGG C^C[GA][CT]GG
Eco81I 360 CCTGAGG CC^T[ACGT]AGG
EcoNI 356 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 253 CCATGG C^C[AT][AT]GG
HhaI 435 GCGC GCG^C
HinP1I 435 GCGC G^CGC
HincII 586 GTTAAC GT[CT]^[GA]AC
HindII 586 GTTAAC GT[CT]^[GA]AC
HpaI 586 GTTAAC GTT^AAC
HphI 90 GGTGA GGTGA(8/7)
MaeI 617 CTAG C^TAG
MaeIII 613 GTAAC ^GT[ACGT]AC
MamI 538 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 74 TCTTC GAAGA(8/7)
MflI 552 GGATCT [GA]^GATC[CT]
MslI 84 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 516 CAGCTG C[AC]G^C[GT]G
NcoI 253 CCATGG C^CATGG
PleI 110 GAGTC GAGTC(4/5)
PvuII 516 CAGCTG CAG^CTG
RcaI 339 TCATGA T^CATGA
SacI 304 GAGCTC GAGCT^C
Sau96I 423 GGACC G^G[ACGT]CC
SpeI 616 ACTAGT A^CTAGT
SphI 689 GCATGC GCATG^C
StyI 253 CCATGG C^C[AT][AT]GG
TaqI 7 TCGA T^CGA
Tsp509I 684 AATT ^AATT
TthHB8I 7 TCGA T^CGA
XhoII 552 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanI 133 GGCACC G^G[CT][GA]CC
196 GGCACC
BcnI 414 CCGGG CC^[GC]GG
713 CCCGG
BsgI 430 GTGCAG GTGCAG(16/14)
457 GTGCAG
BsiYI 356 CCTTCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
414 CCGGGCAGTGG
BslI 356 CCTTCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
414 CCGGGCAGTGG
BsmFI 285 GTCCC GGGAC(10/14)
711 GTCCC
EaeI 572 TGGCCG [CT]^GGCC[GA]
725 CGGCCA
HapII 414 CCGG C^CGG
714 CCGG
HpaII 414 CCGG C^CGG
714 CCGG
MseI 587 TTAA T^TAA
682 TTAA
MspI 414 CCGG C^CGG
714 CCGG
NciI 414 CCGGG CC^[GC]GG
713 CCCGG
NspI 238 ACATGT [GA]CATG^[CT]
689 GCATGC
TfiI 641 GAATC G^A[AT]TC
665 GAATC
Tru9I 587 TTAA T^TAA
682 TTAA
TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^
419 CAGTG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI AspHI BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MboI
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI TseI